Mapping the (Invisible) Salaried Woman Architect: the Australian Parlour Research Project

Since the 1970s, feminist historians and polemicists have struggled to uncover the ordinary lives of women. They believe that gender ideals and biases are a critical part of the weft and weave of daily life. But the quotidian has been a restricted field in our discipline, often used to define a particular building type rather than the lives of architects. For example, we know little about the workdays of professionals or their labour in the workplace. The architectural office - its daily transactions and everyday culture - remains obscure. Even when represented in histories of the profession, the architectural office is filtered through a top-down lens trained on practice directors. The labour and lives of architecture’s male and female employees is unexplored terrain, but we could begin with the demographics: up to three-quarters of Australian women in architecture are salaried workers, continuing a historical trend. In the past, women generally worked for others. The gendering of salaried architectural workers raises questions about the relationship between gender and office work. Feminist historians and theorists have suggested that the office plays a role in forming gender ideals and practices. This paper endeavours to critically describe the lives and labour of women architects at the office, using survey and interview data from a large-scale Australian research project, publicly known through its website Parlour. This research inquires into gender disadvantage and investigates how gender ideals and norms shape the culture of the architectural workplace. The project’s research questions, evidence and explanations form the basis of this essay. The Parlour project is an ongoing platform for sharing information and research, but it gives particular voice to women’s experience in architecture, an experience largely shaped by salaried employment, studentship and the ownership of small practices

The TAM receptor family in multiple myeloma

Multiple myeloma (MM) is a haematological malignancy of antibody secreting monoclonal plasma cells (PCs) in the bone marrow (BM). MM PC growth and survival is supported by other cells within the BM microenvironment including osteoblasts, osteoclasts, bone marrow stromal cells and immune cells. Paracrine signalling between MM PCs and these other cell types is mediated by an array of cytokines, receptors and adhesion molecules. The TAM receptor family, Tyro3, Axl and Mer represent a distinct family of tyrosine kinase cell surface receptors, which have been implicated in the pathogenesis of cancers including MM. The studies presented in this thesis utilised single TAM receptor expressing 5TGM1 murine myeloma cell lines to further elucidate the roles of Axl and Mer in MM. The CRISPR Cas9 system and retroviral transduction were used to generate a 5TGM1 cell line expressing no TAM receptors (5TGM1 EV), a 5TGM1 cell line expressing only Axl (5TGM1 Axl) and a 5TGM1 cell line expressing only Mer (5TGM1 Mer). Dormant MM PCs that reside long term in the bone marrow of patients can be reactivated following therapy, giving rise to disease relapse. Given that Axl was highly expressed by dormant 5TGM1 MM cells in previous studies, the present study sought to determine whether high Axl expression alone was sufficient to initiate and maintain 5TGM1 MM cell dormancy. Features of dormancy in 5TGM1 Axl compared to 5TGM1 EV cells were assessed in vitro using cell cycle analysis and labelling with the heritable dye, DiD. 5TGM1 Axl and 5TGM1 EV cells were also inoculated into the C57BL/KaLwRij murine model of MM, and tumour burden was assessed. These studies provided no evidence that high Axl expression in 5TGM1 cells promote features of dormancy in vitro or in vivo when compared to the 5TGM1 EV cell line that does not express Axl. Studies in this thesis revealed that 5TGM1 Mer cells produce significantly greater myeloma tumour burden in vivo in comparison to 5TGM1 EV cells following intravenous inoculation into the C57BL/KaLwRij mouse model. However, following inoculation of cells directly into the bone marrow, Mer expression did not produce an increase in tumour burden. Additionally, when 5TGM1 Mer cells and 5TGM1 EV cells were inoculated into the immune compromised NSG mouse model, Mer expression had no effect on tumour burden. Immune checkpoint proteins PD-L1, Galectin 9 and PVR were upregulated in 5TGM1 Mer cells compared to 5TGM1 EV cells at the mRNA level. These findings indicate that the mechanism of action of Mer in potentiating MM tumour burden may be through increased 5TGM1 BM homing and regulating expression levels of immune checkpoint molecules in myeloma cells. Future studies should aim to fully characterise the possible role of Mer in MM immune suppression. Given that Mer and its ligand Gas6 are widely expressed by MM PCs of myeloma patients, Mer represents an attractive therapeutic target to limit MM disease progression.Thesis (Ph.D.) -- University of Adelaide, School of Biomedicine, 202

Genetic correlations between wellbeing, depression and anxiety symptoms and behavioral responses to the emotional faces task in healthy twins

© 2018 Elsevier BV. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ This author accepted manuscript is made available following 12 month embargo from date of publication (March 2018) in accordance with the publisher’s archiving policyCurrently there is a very limited understanding of how mental wellbeing versus anxiety and depression symptoms are associated with emotion processing behaviour. For the first time, we examined these associations using a behavioural emotion task of positive and negative facial expressions in 1668 healthy adult twins. Linear mixed model results suggested faster reaction times to happy facial expressions was associated with higher wellbeing scores, and slower reaction times with higher depression and anxiety scores. Multivariate twin modelling identified a significant genetic correlation between depression and anxiety symptoms and reaction time to happy facial expressions, in the absence of any significant correlations with wellbeing. We also found a significant negative phenotypic relationship between depression and anxiety symptoms and accuracy for identifying neutral emotions, although the genetic or environment correlations were not significant in the multivariate model. Overall, the phenotypic relationships between speed of identifying happy facial expressions and wellbeing on the one hand, versus depression and anxiety symptoms on the other, were in opposing directions. Twin modelling revealed a small common genetic correlation between response to happy faces and depression and anxiety symptoms alone, suggesting that wellbeing and depression and anxiety symptoms show largely independent relationships with emotion processing at the behavioral level

'Singing for the Brain': a qualitative study exploring the health and well-being benefits of singing for people with dementia and their carers

Dementia has detrimental effects on cognitive, psychological and behavioural functioning, as well as significant impact on those who provide care. There is a need to find suitable psychosocial interventions to help manage the condition, enhance well-being, and to provide support for caregivers. This study explored the impact of Singing for the Brain™, an intervention based on group singing activities developed by The Alzheimer’s Society for people with dementia and their carers. This qualitative study used semi-structured interviews with people with dementia and their carers. Ten interviews involving 20 participants were analysed thematically. Social inclusiveness and improvements in relationships, memory and mood were found to be especially important to participants. As well as enjoying the sessions, participants found that attending Singing for the Brain™ helped in accepting and coping with dementia

Heritable components of the human fecal microbiome are associated with visceral fat

Background: Variation in the human fecal microbiota has previously been associated with body mass index (BMI). Although obesity is a global health burden, the accumulation of abdominal visceral fat is the specific cardio-metabolic disease risk factor. Here, we explore links between the fecal microbiota and abdominal adiposity using body composition as measured by dual-energy X-ray absorptiometry in a large sample of twins from the TwinsUK cohort, comparing fecal 16S rRNA diversity profiles with six adiposity measures.Results: We profile six adiposity measures in 3666 twins and estimate their heritability, finding novel evidence for strong genetic effects underlying visceral fat and android/gynoid ratio. We confirm the association of lower diversity of the fecal microbiome with obesity and adiposity measures, and then compare the association between fecal microbial composition and the adiposity phenotypes in a discovery subsample of twins. We identify associations between the relative abundances of fecal microbial operational taxonomic units (OTUs) and abdominal adiposity measures. Most of these results involve visceral fat associations, with the strongest associations between visceral fat and Oscillospira members. Using BMI as a surrogate phenotype, we pursue replication in independent samples from three population-based cohorts including American Gut, Flemish Gut Flora Project and the extended TwinsUK cohort. Meta-analyses across the replication samples indicate that 8 OTUs replicate at a stringent threshold across all cohorts, while 49 OTUs achieve nominal significance in at least one replication sample. Heritability analysis of the adiposity-associated microbial OTUs prompted us to assess host genetic-microbe interactions at obesity-associated human candidate loci. We observe significant associations of adiposity-OTU abundances with host genetic variants in the FHIT, TDRG1 and ELAVL4 genes, suggesting a potential role for host genes to mediate the link between the fecal microbiome and obesity.Conclusions: Our results provide novel insights into the role of the fecal microbiota in cardio-metabolic disease with clear potential for prevention and novel therapies

Major depressive disorder and schizophrenia are associated with a disturbed experience of temporal memory

Background Disturbances in ‘psychological time’ are frequently reported in major depressive disorder (MDD) and schizophrenia. If one accepts the suggestion that the experience of the dimensions of time, past-present-future, are not inseparable then a disturbance in episodic memory is implicated. Episodic memory allows us to make sense of the world and our place within it by constructing a temporal context and temporal flow between events. These temporal representations are disordered in schizophrenia, but whether this is reflected in MDD is not known. Temporal-order memory deficits can be explained by two hypotheses. The prefrontal-organisational hypothesis suggests that deficits result from a breakdown in processes involved in encoding, retrieval, monitoring and decision-making. Whereas the hippocampal-mnemonic theory suggests that item-encoding, and inter-item associative encoding contribute to temporal-order memory. Methods New learning, recency judgments and executive function were investigated in 14 MDD patients, 15 schizophrenia patients and 10 healthy volunteers (HVs). Results Relative to HVs, both MDD and schizophrenia made more temporal errors despite achieving 100% learning. Deficits in executive function and item-recognition were present in both psychiatric groups, but executive function correlated to temporal errors in MDD only, and item-recognition to new learning in schizophrenia only. Limitations MDD and schizophrenia patients were taking medication Conclusions Temporal-ordering deficits are evident in both MDD and schizophrenia, and whilst the disruption of organisational and mnemonic processes appears to be ubiquitous, preliminary evidence from the correlational analysis suggests prefrontal problems are implicated in MDD temporal-order deficits, whereas hippocampal are more associated to temporal-order memory deficits in schizophrenia

An integrated approach to processing WHO-2016 verbal autopsy data : the InterVA-5 model

Acknowledgements We are grateful for technical discussions with Dr. Erin K. Nichols. Funding There was no specific funding, other than authors’ time at their institutions, for this work. Publication costs were funded by the Health Systems Research Initiative from the Department for International Development (DFID)/ Medical Research Council (MRC)/Wellcome Trust/Economic and Social Research Council (ESRC) (MR/P014844/1). Availability of data and materials The software, demonstration material, datasets and code supporting the conclusions of this article are freely available in the GitHub repository https://github.com/peterbyass/InterVA-5Peer reviewedPublisher PD

Why, school, why? Gifted students with academic failure.

Neste estudo de caso, de caráter descritivo-interpretativo, pretende-se identificar fatores de desenvolvimento e (in)sucesso escolar de jovens com características de sobredotação, na escolaridade obrigatória (12 a 16 anos). As narrativas apresentadas pelos investigadores revelam as relações entre características individuais e orientações escolares nem sempre aceites. A análise clarifica o tédio, frustração e fracasso que enfrentam estes alunos no cotidiano, tomada de decisão de alheamento, confronto ou ‘crise de identidade’ para a homogeneização e cumprimento de tarefas rotineiras que a escola oferece. Revelam ainda o relevo dos papéis do professor na diferenciação curricular e, em certos casos, no sucesso escolar dos estudantes.In this case study of a descriptive and interpretive nature it is intended to identify developmental factors and the success/failure at school of gifted youngsters (aged 12 to 16 years old) in compulsory education. The narratives presented by researchers show that the relationships between individual characteristics and school guidance are not always accepted. The analysis clarifies the boredom, frustration and failure facing these students in their everyday life, absent-minded decision-making, opposition or ‘identity crisis’ related to their standardization and compliance with routine tasks that the school requires today. They also reveal the importance of the role of the teacher in terms of curriculum differentiation and, in some cases, the academic success of the students.CIEC - Centro de Investigação em Estudos da Criança, IE, UMinho (UI 317 da FCT), Portugal. Fundos Nacionais através da FCT (Fundação para a Ciência e a Tecnologia) e cofinanciado pelo Fundo Europeu de Desenvolvimento Regional (FEDER) através do COMPETE 2020 – Programa Operacional Competitividade e Internacionalização (POCI) com a referência POCI-01-0145-FEDER-007562info:eu-repo/semantics/publishedVersio

Exon expression arrays as a tool to identify new cancer genes

Background: Identification of genes that are causally implicated in oncogenesis is a major goal in cancer research. An estimated 10-20% of cancer-related gene mutations result in skipping of one or more exons in the encoded transcripts. Here we report on a strategy to screen in a global fashion for such exon-skipping events using PAttern based Correlation (PAC). The PAC algorithm has been used previously to identify differentially expressed splice variants between two predefined subgroups. As genetic changes in cancer are sample specific, we tested the ability of PAC to identify aberrantly expressed exons in single samples. Principal Findings: As a proof-of-principle, we tested the PAC strategy on human cancer samples of which the complete coding sequence of eight cancer genes had been screened for mutations. PAC detected all seven exon-skipping mutants among 12 cancer cell lines. PAC also identified exon-skipping mutants in clinical cancer specimens although detection was compromised due to heterogeneous (wild-type) transcript expression. PAC reduced the number candidate genes/exons for subsequent mutational analysis by two to three orders of magnitude and had a substantial true positive rate. Importantly, of 112 randomly selected outlier exons, sequence analysis identified two novel exon skipping events, two novel base changes and 21 previously reported base changes (SNPs). Conclusions: The ability of PAC to enrich for mutated transcripts and to identify known and novel genetic changes confirms its suitability as a strategy to identify candidate cancer genes