Assembly and Dynamics of Myofibrils

We review some of the problems in determining how myofibrils may be assembled and just as importantly how this contractile structure may be renewed by sarcomeric proteins moving between the sarcomere and the cytoplasm. We also address in this personal review the recent evidence that indicates that the assembly and dynamics of myofibrils are conserved whether the cells are analyzed in situ or in tissue culture conditions. We suggest that myofibrillogenesis is a fundamentally conserved process, comparable to protein synthesis, mitosis, or cytokinesis, whether examined in situ or in vitro

Precipitable Water Vapor Retrieval Based on GNSS Data and Its Application in Extreme Rainfall

Water vapor plays a crucial role in maintaining global energy balance and water cycle, and it is closely linked to various meteorological disasters. Precipitable water vapor (PWV), as an indicator of variations in atmospheric water vapor content, has become a key parameter for meteorological and climate monitoring. However, due to limitations in observation costs and technology, traditional atmospheric monitoring techniques often struggle to accurately capture the distribution and variations in space–time water vapor. With the continuous advancement of Global Navigation Satellite System (GNSS) technology, ground-based GNSS monitoring technology has shown rapid development momentum in the field of meteorology and is considered an emerging monitoring tool with great potential. Hence, based on the GNSS observation data from July 2023, this study retrieves PWV using the Global Pressure and Temperature 3 (GPT3) model and evaluates its application performance in the “7·31” extremely torrential rain event in Beijing in 2023. Research has found the following: (1) Tropospheric parameters, including the PWV, zenith tropospheric delay (ZTD), and zenith wet delay (ZWD), exhibit high consistency and are significantly affected by weather conditions, particularly exhibiting an increasing-then-decreasing trend during rainfall events. (2) Through comparisons with the PWV values through the integration based on fifth-generation European Centre for Medium-Range Weather Forecasts (ERA-5) reanalysis data, it was found that results obtained using the GPT3 model exhibit high accuracy, with GNSS PWV achieving a standard deviation (STD) of 0.795 mm and a root mean square error (RMSE) of 3.886 mm. (3) During the rainfall period, GNSS PWV remains at a high level (>50 mm), and a strong correlation exists between GNSS PWV and peak hourly precipitation. Furthermore, PWV demonstrates the highest relative contribution in predicting extreme precipitation, highlighting its potential value for monitoring and predicting rainfall events

A radiomics model fusing clinical features to predict microsatellite status preoperatively in colorectal cancer liver metastasis

Abstract Purpose To study the combined model of radiomic features and clinical features based on enhanced CT images for noninvasive evaluation of microsatellite instability (MSI) status in colorectal liver metastasis (CRLM) before surgery. Methods The study included 104 patients retrospectively and collected CT images of patients. We adjusted the region of interest to increase the number of MSI-H images. Radiomic features were extracted from these CT images. The logistic models of simple clinical features, simple radiomic features, and radiomic features with clinical features were constructed from the original image data and the expanded data, respectively. The six models were evaluated in the validation set. A nomogram was made to conveniently show the probability of the patient having a high MSI (MSI-H). Results The model including radiomic features and clinical features in the expanded data worked best in the validation group. Conclusion A logistic regression prediction model based on enhanced CT images combining clinical features and radiomic features after increasing the number of MSI-H images can effectively identify patients with CRLM with MSI-H and low-frequency microsatellite instability (MSI-L), and provide effective guidance for clinical immunotherapy of CRLM patients with unknown MSI status

Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis. Cell Motil Cytoskeleton. 2005; 62:35–47. [PubMed: 16080205

To test different models of myofibrillogenesis, we followed live cells expressing Green Fluorescent Proteins ligated to either actin or alpha-actinin and analyzed stress fibers, premyofibrils, and myofibrils in quail myotube cultures. Actin filaments in the three types of fibers were compared by analyzing the effects of Latrunculin-A (Lat-A), a monomeric actin binding macrolide drug (M.W. ¼ 422 Daltons), on stress fibers in fibroblasts and on myofibrils in skeletal myotubes in the same culture. Lat-A, at low concentrations (0.2 mM), induced the loss of stress fibers in fibroblasts within a few hours and within 10 min when Lat-A was increased to 1.0 mM. The effect was reversible with reformation of the stress fibers when the drug was removed. In contrast to the Lat-A induced disassembly of stress fibers in fibroblasts, assembling myofibrils in the skeletal muscle cells were not affected by 1.0-mM concentrations of Lat-A. With increasing concentrations of Lat-A (up to 5 mM), and increasing incubation times, however, the drug induced premyofibrils, the precursors of mature myofibrils, to disassemble and the accumulation of mature myofibrils to be halted. Removal of the drug led to the reformation of premyofibrils and the resumption of myofibrillogenesis in the spreading edges of the myotubes. In contrast, the mature myofibrils in the central shaft of the myotubes were stable in doses of Lat-A as high as 50 mM. The newly assembled mature myofibrils located adjacent to the premyofibrils at the ends and sides of the myotube were intermediate in sensitivity to Lat-A, disassembling when exposed to 10 mM Lat-A for one hour. To determine how a change in the actin filaments during myofibrillogenesis might confer greater resistance to depolymerization by Lat-A, we stained the myotubes with an antibody directed against CapZ, a protein that blocks the release of monomer actin from the barbed ends of actin filaments. CapZ was absent from premyofibrils. It was distributed uniformly along nascent myofibrils where F-actin was unstriated, and was localized in a clearly striated Z-band pattern in the mature myofibrils where F-actin patterns were fully striated. These Lat-A and CapZ results are discussed in the context of various models of myofibrillogenesis. Cell Motil

Changes of absorption spectra during heat-induced denaturation of Photosystem II core antenna complexes CP43 and CP47: revealing the binding states of chlorophyll molecules in these two complexes

AbstractThe Photosystem II (PSII) core antenna complexes, CP43 and CP47, were prepared from spinach (Spinacia oleracea L.). The absorption spectra in the red region at room temperature were recorded for the PSII core antenna samples after increased temperature treatment (up to 80°C). Derivative and difference spectra revealed the existence of two groups of chlorophyll a (Chl a) molecules in both CP43 and CP47. The one with the absorption peak in the shorter wavelength region was designated as CP43-669 and CP47-669, while the other with the absorption peak in the longer wavelength region was designated as CP43-682 and CP47-680. The results of the thermal treatment experiment demonstrated that CP43-669 and CP47-669 may exist as monomers of Chl a and that their binding sites on the polypeptides are insensitive to thermal treatment, whereas CP43-682 and CP47-680 may exist as dimers or multimers of Chl a and their binding regions in the polypeptide chains are more sensitive to heat treatment. The excitation energy transfer mechanism between these two different groups of Chl a molecules is also analyzed