Etude du flux salivaire de glucose et des transporteurs de l'hexose exprimés dans les parotides de sujets sains et diabétiques

Previous epidemiological studies have shown that the diabetic population has a poor oral status compared to healthy subjects. While changes of the local microcirculation observed during diabetes can somehow explain periodontal pathologies this hypothesis does not stand for the carious decay. We propose the hypothesis that the increase of carious incidence might be linked to changes in the salivary composition, such as an increase of salivary glucose concentrations.The amount of glucose in saliva has been widely discussed in various scientific articles. Subsequently, we performed an initial study that allowed us to confirm that the concentration and excretion of glucose in the saliva were higher in diabetic patients compared to normal patients. In diabetic patients, the relative increase in salivary glucose concentration is even comparable to the rise of blood glucose.We conducted experimental studies in animals in order to investigate the presence and the level of expression of glucose transporters (GLUT1, GLUT2, GLUT4, SGLT1) in rat parotid glands. Through immunohistochemical labeling techniques, gene expression studies and protein expression studies, we demonstrated that GLUT1, GLUT4 and SGLT1 were found in rat parotid glands of normal and diabetic rats. Functional studies targeting the activity and the flux through those glucose transporters strengthened our hypothesis on the potential role of glucose transporter in salivary glucose.This original work introduce for the first time the hypothesis of a secretion mechanism of glucose by the salivary glands through GLUT1 & SGLT1 transporters, the first one being located on the apical and baso-lateral membranes of acinar cells, while the second one is located on the baso-lateral membrane. Secretion of glucose by the parotid gland is no longer at any doubt even though further studies must now specify the specific mechanisms.Doctorat en Sciences dentairesinfo:eu-repo/semantics/nonPublishe

Evaluation of the impact of regular control visits on dmft and DMFT indexes

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Comparison of PI and DPSI indices in diabetic and normal subjects

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Biodentine used as a pulp-capping agent in primary pig teeth

Purpose: The purpose of this study was to assess and compare, in primary pig teeth, the pulp response after a pulpotomy using either Biodentine (a new tricalcium-silicate cement), white mineral trioxide aggregate (WMTA), or formocresol (FC) and repeat the same after direct pulp capping using either Biodentine, WMTA, or calcium hydroxide. Methods: A total of 180 primary teeth from 9 healthy 4-month-old female pigs were divided into 3 experimental periods (7, 28, and 90 days) for each material used for the pulpotomy and direct pulp capping treatments. Seven, 28, and 90 days later, the animals were euthanized and the specimens were prepared for histological examination and evaluation. The data collected from the histological examinations were statistically analyzed using Kruskal-Wallis and Dunn multiple comparison tests. Results: In pulpotomy groups, there was a significant difference between Biodentine and WMTA vs FC in terms of inflammatory cell response and hard tissue formation. In direct pulp capping groups, there was only a significant difference between Biodentine and calcium hydroxide in terms of hard tissue formation in a 7-day period. Conclusions: Biodentine and white mineral trioxide aggregate are both suitable, biocompatible materials for pulp capping in primary teeth of pigs.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Glucose transport by acinar cells in rat parotid glands.

Salivary glucose is often considered as being from glandular origin. Little information is available, however, on the possible role of glucose transporters in the secretion of the hexose by salivary glands. The major aim of the present study was to investigate the expression and localization of several distinct glucose transporters in acinar cells of rat parotid glands.Journal ArticleResearch Support, Non-U.S. Gov'tSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Presence of GLUT4 and SLGT1 in human salivary glands: a primary study

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Quantitative and qualitative alterations of intestinal mucins in BioBreeding rats.

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Presence of Glut4 and SGLT1 in human salivary glands: a preliminary study

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Altered fatty acid pattern of phospholipids and triglycerides in the submandibular gland of omega3-depleted rats.

Alteration of the phospholipid (PL) and triglyceride (TG) fatty acid pattern was recently documented in several organs of rats depleted in long-chain polyunsaturated omega3 fatty acid (omega3 rats). This study extends such a knowledge to the submandibular gland. The total PL and TG content of the salivary gland was not different in control and omega3 rats. The sole omega3 fatty acids found in omega3 rats (C22:5omega3 and C22:6omega3) were present at levels 3-12 times lower than in control rats. The C22:5omega3/C22:6omega3 ratio was increased threefold in omega3 rats. The PL and TG C16:0/C16:1omega7 and C18:0/C18:1omega9 ratios were decreased in omega3 rats. The conversion of C18:2omega6 to C20:4omega6 and C22:4omega6 appeared facilitated in the omega3 rats. Some of these rats were injected intravenously, 60-120 min before killing, with either a medium-chain triglyceride:fish oil emulsion or a control medium-chain triglyceride:olive oil emulsion. The former emulsion increased the PL C22:5omega3 and C22:6omega3 content and prevented the age-related decrease in C16:0/C16:1omega7 and C18:0/C18:1omega9 ratios otherwise also recorded in PL. In conclusion, these findings document an increased activity of Delta9-desaturase, a more efficient conversion of C18:2omega6 to its metabolites, and an impaired generation of C22:6omega3 from C22:5omega3 in omega3 rats.Journal ArticleResearch Support, Non-U.S. Gov'tFLWINinfo:eu-repo/semantics/publishe