Balancing the Initiation and Molecular Recognition Capabilities of Eosin Macroinitiators of Polymerization-Based Signal Amplification Reactions

Coupling polymerization initiators to molecular recognition events provide the ability to amplify these events and detect them using the formation of a cross-linked polymer as an inexpensive readout that is visible to the unaided eye. The eosin-tertiary amine co-initiation system, activated by visible light, has proven utility in this context when an average of three eosin molecules are coupled to a protein detection reagent. The present work addresses the question of how detection sensitivity is impacted when the number of eosin molecules per binding event increases in the range of two to fifteen. Unlike in other initiation systems, a non-monotonic relationship is observed between the number of initiators per binding event and the observed detection sensitivity.Burroughs Wellcome Fund (Career Award at the Scientific Interface)Massachusetts Institute of Technology. James H. Ferry Fund for Innovation in Research Educatio

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.David H. Koch Institute for Integrative Cancer Research at MIT (First-year Graduate Fellowship)National Science Foundation (U.S.). Graduate Research FellowshipBurroughs Wellcome Fund (Career Award at the Scientific Interface)National Institute of Environmental Health Sciences (Grant P30-ES002109)Massachusetts Institute of Technology. James H. Ferry Fund for Innovation in Research Educatio

Single-band and Dual-band Beam Switching Systems and Offset-fed Beam Scanning Reflectarray

The reflectarray has been considered as a suitable candidate to replace the conventional parabolic reflectors because of its high-gain, low profile, and beam reconfiguration capability. Beam scanning capability and multi-band operation of the microstrip reflectarray have been main research topics in the reflectarray design. Narrow bandwidth of the reflectarray is the main obstacle for the various uses of the reflectarray. The wideband antenna element with a large phase variation range and a linear phase response is one of the solutions to increase the narrow bandwidth of the reflectarray. A four beam scanning reflectarray has been developed. It is the offset-fed microstrip reflectarray that has been developed to emulate a cylindrical reflector. Unlike other microstrip reflectarrays which integrates phase tuning devices such as RF MEMS switches and another phase shifters to the reflectarray elements and control the reflected phase, the beam scanning capability of the reflectarray is implemented by a phased array feed antenna. This method can reduce the complexity of the design of the beam switching reflectarray. A simple method has been investigated to develop multi-band elements in this dissertation. In approach to increase the coverage of the operation bands, a six-band reflectarray has been developed with two layers. Each layer covers three frequency bands. A Butler matrix is one of the useful beamforming networks for a phased array antenna. A Double-Sided Parallel-Strip Line (DSPSL) is adapted for the feeding network of eight array elements. The DSPSL operate very well to feed the microstrip antenna array over the bandwidth to reduce the sidelobe level and a high gain. In another topic of a Butler matrix, a dual-band Butler matrix has been proposed for multi-band applications. A modified Butler matrix is used to reduce a size and a sidelobe level. The bandwidth of the microstrip antenna is inherently small. A broadband circularly polarized microstrip antenna with dual-offset feedlines is introduced in this dissertation. Aperture-coupled feed method is used to feed the stacked patch antennas and a slotcoupled directional coupler is used for the circularly polarized operation. The research presented in this dissertation suggests useful techniques for a beam scanning microstrip reflectarray, phased array antenna, and wideband antenna designs in the modern wireless communication systems

A Fast and Scalable Re-routing Algorithm based on Shortest Path and Genetic Algorithms J. Lee, J. Yang Jungkyu Lee

This paper presents a fast and scalable re-routing algorithm that adapts to dynamically changing networks. The proposed algorithm, DGA, integrates Dijkstra’s shortest path algorithm with the genetic algorithm. Dijkstra’s algorithm is used to define the predecessor array that facilitates the initialization process of the genetic algorithm. Then the genetic algorithm keeps finding the best routes with appropriate genetic operators under dynamic traffic situations. Experimental results demonstrate that DGA produces routes with less traveling time and computational overhead than pure genetic algorithm-based approaches as well as Dijkstra’s algorithm in largescale routing problems

Mitigating Biases for Instruction-following Language Models via Bias Neurons Elimination

Instruction-following language models often show undesirable biases. These undesirable biases may be accelerated in the real-world usage of language models, where a wide range of instructions is used through zero-shot example prompting. To solve this problem, we first define the bias neuron, which significantly affects biased outputs, and prove its existence empirically. Furthermore, we propose a novel and practical bias mitigation method, CRISPR, to eliminate bias neurons of language models in instruction-following settings. CRISPR automatically determines biased outputs and categorizes neurons that affect the biased outputs as bias neurons using an explainability method. Experimental results demonstrate the effectiveness of our method in mitigating biases under zero-shot instruction-following settings without losing the model's task performance and existing knowledge. The experimental results reveal the generalizability of our method as it shows robustness under various instructions and datasets. Surprisingly, our method can mitigate the bias in language models by eliminating only a few neurons (at least three).Comment: accepted to ACL 202

Compositions and methods for detecting mycobacterium

The present disclosure provides methods of detecting mycobacterium in an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the methodsThe present disclosure provides methods of detecting mycobacterium in an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the method

The association of renin–angiotensin system blockades and pneumonia requiring admission in patients with COPD

BACKGROUND: The hallmark of COPD is chronic airway inflammation, which may be mediated by renin–angiotensin system. The renin–angiotensin system blockers such as angiotensin-converting enzyme inhibitors (ACEi) and angiotensin II receptor blockers (ARBs) have exhibited anti-inflammatory and immunomodulatory effects in patients with various diseases. We explored the effects of ACEi and ARBs on the risk of pneumonia in patients with COPD. METHODS: A nested case–control study was performed on COPD patients recruited from January 2010 to August 2013 in two referral hospitals in Korea. A total of 130 COPD patients admitted with pneumonia were included, and 245 COPD patients without pneumonia were selected as controls from a total of 1,646 such patients. Controls were matched with test patients by age, sex, and severity of airflow limitation. The effects of ACEi/ARBs use on the odds ratio (OR) for the development of pneumonia were tested through conditional logistic regression. RESULTS: Elderly patients (over 70 years of age) constituted ~30% of each group; most of the patients were male (85%). Of the COPD patients with pneumonia, 21.5% had taken ACEi/ARBs for a mean of 9.8 months (standard deviation ±3.5 months). The proportions of ACEi/ARBs users and the mean duration of such use did not differ when compared to those of the control patients (26.9%, P=0.25; 9.6±3.6 months, P=0.83). Univariate analyses indicated that the use of ACEi/ARBs was not associated with a decreased risk of pneumonia (OR =0.70, 95% confidence interval 0.41–1.23, P=0.21), whereas both a history of pulmonary tuberculosis (OR =1.85, 95% confidence interval 1.12–3.06, P=0.02) and exposure to systemic steroids (OR =2.33, 95% confidence interval 1.28–4.23, P=0.005) did show an association. After adjustment for a history of tuberculosis, comorbid chronic renal disease, and exposure to corticosteroids, ACEi/ARBs reduced the risk of pneumonia in COPD patients (OR =0.51, 95% confidence interval 0.27–0.98, P=0.04). CONCLUSION: This study revealed that the use of ACEi/ARBs was associated with reducing the risk of pneumonia in patients with COPD. Further prospective studies are necessary to confirm the protective effect of ACEi/ARBs and elucidate the underlying mechanisms in COPD patients