Elektroforetska analiza proteina iz spolnih organa i kutikule glista Ascaris lumbricoides i Ascaris suum

Ascaris lumbricoides and Ascaris suum were analysed for similarities and differences in the banding patterns of proteins extracted from the body wall (BW) and reproductive organs (ROS). Ascaris lumbricoides and A. suum manifested considerable similarity in protein profile. The BW of female ascarids manifested 10 common bands, while males had 12 common bands, and of these proteins, at least five were common to both sexes. A comparison of the ROS banding profile between female and male Ascaris lumbricodes and A. suum revealed 11 and 12 common bands, respectively, of which six bands were shared. While the BW and ROS of male ascarids revealed eight shared bands, female ascarids had only two common bands. The detection of 25 kDa, 20-21 kDa and 18 kDa bands in virtually all protein samples assayed suggests their importance as housekeeping molecules and possibly valuable markers. The appreciable similarities in protein banding patterns between human and hog ascarids reflect their close genetic relationship. In view of current findings and earlier documented studies pointing to the zoonotic nature and high probability of cross-infection between human and hog species, control strategies should take into consideration the simultaneous deworming of infected persons and hogs. The likelihood of cross-infection between human and hog Ascaris spp. in the country can be further assessed through surveillance of human and hog ascarids, in places where backyard hog-rearing is still commonly practised. The current electrophoretic protein profiling of Ascaris spp. is the first of its kind in the country.Ascaris lumbricoides i Ascaris suum bili su analizirani radi utvrđivanja sličnosti i razlike u elektroforetskoj pokretljivosti proteina iz njihove kutikule i spolnih organa. U obje vrste dokazana je znatna sličnost proteinskoga profila. Elektroforezom iscrpka kutikule ženki ustanovljeno je deset zajedničkih vrpci za obje vrste, dok je elektroforezom proteina kutikule mužjaka dobiveno 12 zajedničkih vrpci proteina od čega je najmanje pet proteina bilo zajedničko za oba spola. Usporedbom proteinskoga profila spolnih organa ženki i mužjaka obiju vrsta dokazane su također mnoge sličnosti. U proteinskomu profilu vrste Ascaris lumbricoides dokazano je 11 proteinskih traka dok je u proteinskomu profilu vrste Ascaris suum dokazano 12 vrpci, od čega je šest bilo zajedničkih. Elektroforezom proteinskih iscrpaka spolnih organa i kutikule mužjaka dokazano je osam zajedničkih proteinskih vrpci, dok su istom usporedbom proteinskoga profila ženki dokazane samo dvije zajedničke proteinske vrpce. Nalaz proteinskih vrpci od 25 kDa, 20-21 kDa i 18 kDa gotovo u svim pretraženim proteinskim ekstraktima govori o njihovoj velikoj važnosti te mogućoj upotrebi u svojstvu markera. Dokazane sličnosti u sastavu proteinskih ekstrakata u askarida izdvojenih iz ljudi i svinja govori o njihovoj bliskoj genetskoj srodnosti. S obzirom na ove nalaze i ranija istraživanja koja upućuju na zoonotsku prirodu askaridoze i veliku mogućnost križnih invazija ljudi i svinja, treba donijeti kontrolne mjere za istodobno dehelmintizaciju invadiranih ljudi i svinja. Vjerojatnost križne invazije ljudi i svinja vrstama roda Ascaris treba procijeniti na osnovi pretraga na askaridozu u mjestima gdje se svinje još uvijek drže po dvorištima. Ovo je prva analiza proteinskoga profila vrsta roda Ascaris na Filipinima

Ascaris lumbricoides and Ascaris suum: A comparison of electrophoretic banding patterns of protein extracts from the reproductive organs and body wall

Ascaris lumbricoides and Ascaris suum were analysed for similarities and differences in the banding patterns of proteins extracted from the body wall (BW) and reproductive organs (ROS). Ascaris lumbricoides and a. suum manifested considerable similarity in protein profile. The BW of female ascarids manifested 10 common bands, while males had 12 common bands, and of these proteins, at least five were common to both sexes. A comparison of the ROS banding profile between female and male Ascaris lumbricodes and A. suum revealed 11 and 12 common bands, respectively, of which six bands were shared. While the BW and ROS of male ascarids revealed eight shared bands, female ascarids had only two common bands. The detection of 25 kDa, 20-21 kDa and 18 kDa bands in virtually all protein samples assayed suggests their importance as housekeeping molecules and possibly valuable markers. The appreciable similarities in protein banding patterns between human and hog ascarids reflect their close genetic relationship. In view of current findings and earlier documented studies pointing to the zoonotic nature and high probability of cross-infection between human and hog species, control strategies should take into consideration the simultaneous deworming of infected persons and hogs. The likelihood of cross-infection between human and hog Ascaris spp. in the country can be further assessed through surveillance of human and hog ascarids, in places where backyard hog-rearing is still commonly practised. The current electrophoretic protein profiling of Ascaris spp. is the first of its kind in the country