Beta diversity indices of prokaryotes and eukaryotes by <i>Leishmania</i> species.

Beta diversity of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by Leishmania species. There are not significant differences between species groups. (TIF)</p

Geographical sampling locations.

Map illustrating the geographical locations from which samples were collected in Colombia. Includes an explanation of the sample codification system, which is based on the initials of the collection sites (B = Bonza, G = Guaviare, M = Medellín), the sample type (L = Skin Lesion, S = Healthy Skin) and a unique numerical identifier (e.g., BL-13). The map was constructed using QGIS version 2.18.7. Basemap: Elevation/World_Hillshade https://bit.ly/3vVQ1lL; Sources: Esri, Airbus DS, USGS, NGA, NASA, CGIAR, N Robinson, NCEAS, NLS, OS, NMA, Geodatastyrelsen, Rijkswaterstaat, GSA, Geoland, FEMA. (TIF)</p

Relative abundance of sub-grouped prokaryotic and eukaryotic families and species.

(A). Boxplots presenting alterations in the relative abundance of prokaryotic phyla within samples grouped into healthy skin (Healthy) and lesion skin (Lesion). Subsequently, the relative abundance of prokaryotic families is displayed, both without (B) and with the subgroup (C) of the most abundant species found on the skin according to the literature. Lastly, shifts in microbiota composition are portrayed for species from the selected subgroup of eukaryotes (D). (*) signify statistically significant differences between groups.</p

Relative abundance of prokaryotic species.

Boxplots illustrating changes in the relative abundance of prokaryotic species within samples categorized by healthy skin (Healthy) and lesion skin (Lesion). There are not significant differences between prokaryotic species groups. (TIF)</p

<i>Leishmania</i> species abundance percentages.

Here are only shown those greater than 5% abundance (in relation to the total reads obtained by Oxford Nanopore Sequencing). (XLSX)</p

Beta diversity indices of prokaryotes and eukaryotes by collection sites.

Beta diversity of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by collection sites (Bonza, Guaviare, Medellín). There are not significant differences between collection sites groups. (TIF)</p

Relative abundance of prokaryotic and eukaryotic phyla, families, and species.

Boxplots illustrating changes in the relative abundance of prokaryotic phyla within samples categorized by healthy skin (Healthy) and lesion skin (Lesion), both without (A) and with the subgroup (B) of the most abundant species found on the skin according to the literature. This is followed by an analysis of changes in the composition of prokaryotic (C) and eukaryotic (D) families. Finally, alterations in relative abundance are demonstrated for species using the subgroup approach for both prokaryotes (E) and eukaryotes (F). (*) denote statistically significant differences between groups.</p

Alpha diversity indices of prokaryotes and eukaryotes by injury body location.

Alpha diversity indices of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by injury body location. There are not significant differences between injury body location groups. (TIF)</p

Alpha diversity indices of prokaryotes and eukaryotes by skin phototypes.

Alpha diversity indices of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by skin phototypes (II, III, IV, V). There are not significant differences between skin phototypes groups. (TIF)</p

Beta diversity indices of prokaryotes and eukaryotes by ulcer size.

Beta diversity of prokaryotes (A) and eukaryotes (B) based on the sample type (healthy skin (Healthy) or the lesion (Lesion)) divided by ulcer size (categorized into quartiles: Q1 = 150mm, Q2 = 151-245mm, Q3 = 246-501mm, Q4 = 502-1963mm). There are not significant differences between ulcer size groups. (TIF)</p