Biodegradation of endocrine disruptors in solid-liquid two-phase partitioning systems by enrichment cultures.

International audienceNaturally occurring and synthetic estrogens and other molecules from industrial sources strongly contribute to the endocrine disruption of urban wastewater. Because of the presence of these molecules in low but effective concentrations in wastewaters, these endocrine disruptors (EDs) are only partially removed after most wastewater treatments, reflecting the presence of these molecules in rivers in urban areas. The development of a two-phase partitioning bioreactor (TPPB) might be an effective strategy for the removal of EDs from wastewater plant effluents. Here, we describe the establishment of three ED-degrading microbial enrichment cultures adapted to a solid-liquid two-phase partitioning system using Hytrel as the immiscible water phase and loaded with estrone, estradiol, estriol, ethynylestradiol, nonylphenol, and bisphenol A. All molecules except ethynylestradiol were degraded in the enrichment cultures. The bacterial composition of the three enrichment cultures was determined using 16S rRNA gene sequencing and showed sequences affiliated with bacteria associated with the degradation of these compounds, such as Sphingomonadales. One Rhodococcus isolate capable of degrading estrone, estradiol, and estriol was isolated from one enrichment culture. These results highlight the great potential for the development of TPPB for the degradation of highly diluted EDs in water effluents

The spectroscopy and H-band imaging of Virgo cluster galaxies (SHIVir) survey: data catalogue and kinematic profiles

The 'Spectroscopy and H-band Imaging of Virgo cluster galaxies' (SHIVir) survey is an optical and near-infrared survey which combines SDSS photometry, deep H-band photometry, and long-slit optical spectroscopy for 190 Virgo cluster galaxies covering all morphological types over the stellar mass range log (M∗/M·) = 7.8-11.5. We present the spectroscopic sample selection, data reduction, and analysis for this SHIVir sample. We have used and optimized the pPXF routine to extract stellar kinematics from our data. Ultimately, resolved kinematic profiles (rotation curves and velocity dispersion profiles) are available for 133 SHIVir galaxies. A comprehensive data base of photometric and kinematic parameters for the SHIVir sample is presented with grizH magnitudes, effective surface brightnesses, effective and isophotal radii, rotational velocities, velocity dispersions, and stellar and dynamical masses. Parameter distributions highlight some bimodal distributions and possible sample biases. A qualitative study of resolved extended velocity dispersion profiles suggests a link between the so-called 'sigma-drop' kinematic profile and the presence of rings in lenticular S0 galaxies. Rising dispersion profiles are linked to early-type spirals or dwarf ellipticals for which a rotational component is significant, whereas peaked profiles are tied to featureless giant ellipticals

High-throughput screening in niche-based assay identifies compounds to target preleukemic stem cells

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