Behavioral parameters (averaged over the day) recorded during the spatial training trials in MR-Tg and MR^CaMKCre mice and their control littermates.

<p>The spatial task consisted of six trials. Data represent mean ± SEM of all trials. For statistics a repeated measures ANOVA was used over the trials. Behavioral parameters that differ significantly; p<0.05: *vs same sex control littermates; ^#female MR^CaMKCre vs MR-Tg; ^$male vs female control littermates of MR^CaMKCre mice.</p

Experimental design of training and memory testing.

<p>Experimental design of training and memory testing.</p

Behavioral parameters recorded during the spatial memory test 24 hours after six spatial training trials in MR-Tg and MR^CaMKCre mice and their control littermates.

<p>Data represent mean ± SEM. Behavioral parameters that differ significantly; p<0.05: *vs same sex control littermates; ^#female MR^CaMKCre vs MR-Tg; ^$male vs female MR^CaMKCre; ^∼duration in exit quadrant vs average duration in other quadrants.</p

Stimulus-response (S-R) task: latency to the exit hole over six training trials on two days.

<p>A) Female MR-Tg mice and controls have short and similar latencies to the exit hole. B) Female MR^CaMKCre mice take more time to locate the exit than controls in trials 3 and 5 on day 1, and in all trials on day 2. C) Male MR^CaMKCre mice take more time to locate the exit than controls on day 2. Bars show mean ± SEM. *p<0.05 vs. control littermates.</p

Spatial exit memory during the first trial of the stimulus-response task on day 1 (one week after the spatial memory test) in MR-Tg and MR^CaMKCre mice and their control littermates.

<p>Data represent mean ± SEM. Behavioral parameters that differ significantly; p<0.05: *vs same sex control littermates; ^#female MR^CaMKCre vs MR-Tg.</p

Percentage of serial hole visits averaged over the trials of the two days of the stimulus-response task.

<p>Both days of the stimulus-response task consisted of six trials. Data represent mean ± SEM of all trials of one day. For statistics a repeated measures ANOVA was used over the trials of one day.</p>*<p>Significantly different between genotypes of the same group over the day; p<0.05.</p

Spatial task: latency to the exit hole over six training trials and spatial memory test.

<p>A) Female MR-Tg mice (n = 19) take less time to find the exit hole than controls (n = 20). B) Female MR^CaMKCre mice take more time to find the exit hole than controls (n = 12 per group). C) Male MR^CaMKCre and control mice (n = 12 per group) have comparable latencies to locate the exit hole. Bars show mean ± SEM. *p<0.05 over trials, vs. control littermates. n.s. = not significant.</p

Behavioral parameters (averaged over the day) recorded during the stimulus-response task in MR-Tg and MR^CaMKCre mice and their control littermates.

<p>Both days of the stimulus-response task consisted of six trials. Data represent mean ± SEM of all trials of one day. For statistics a repeated measures ANOVA was used over the trials of one day. Behavioral parameters that differ significantly; p<0.05: *vs same sex control littermates; ^#female MR^CaMKCre vs MR-Tg; ^$male vs female MR^CaMKCre.</p

DCL knockdown results in a delayed NB tumor growth.

<p>The normalized tumor growth is shown for shDCL-2 (A), shDCL-3 (B) and NC (C) tumors developed from the correspondent Dox-inducible NB cells lines injected subcutaneously in BALB/c athymic nude mice. Tumor growth (A-C) was normalized to the maximum tumor size per group. (D) Average of tumor volume 9, 12 and 14 days after injecting the Dox-inducible NB cells. NC, negative control Dox-inducible NB tumors. shDCL-2 and shDCL-3, Dox-inducible NB tumors that express shRNA against DCL. Error bars, s.e.m. *, <i>P</i><0.05; **, <i>P</i><0.01; ***, <i>P</i><0.001.</p

DCL knockdown results in less mitochondrial colocalization in NB cells.

<p>DCL (green) and mitochondria (red) staining in Dox-inducible NB cells treated with doxycycline revealed less colocalization (yellow) in NB cells with DCL knockdown (shDCL-2 and shDCL-3). Colocalization scores and images of Dox-inducible NB cells treated with vehicle are shown in Figure S4. Mitochondria were stained with 100 nM MitoTraker Orange CMTM Rosamine. Blue, Hoechst staining. NC, negative control Dox-inducible NB cell lines that express scramble shRNA. shDCL-2 and shDCL-3, Dox-inducible NB cell lines that express shRNA against DCL. Scale bars, 10 µm.</p