The Modifications of the Main Façade of the Spanish Senate Palace

In the historical study of the Senate Building façades have been given the interventions taken after the Civil War (1936-1939). The first phase began around 1951, under the responsibility of the architect Ambrós Escanellas who justified the reconstruction of the façades because the plaster and renderings were in dire conditions and broken stone areas affected by frosting. According to the photographic documentation, there was a complete removing of lining facade of the Plaza de la Marina, and the dismantling of all its ornamental elements. It indicated that in the works to be performed, apply a veneer artificial stone "polished arcosite Butsems" made from portland cement according to XRD analysis carried out. It was also expanding the second floor to 1969-1974. In any case the evolution of the fronts are the result of a process to adapt the set to new uses and the currents of modernization. The South front in particular is the most documented by changes in the church of the former Augustinian monastery (1581-1601) to become Courts Salon to 1814 and later Plenary Salon

A novel sequencing-based vaginal health assay combining self-sampling, HPV detection and genotyping, STI detection, and vaginal microbiome analysis

The composition of the vaginal microbiome, including both the presence of pathogens involved in sexually transmitted infections (STI) as well as commensal microbiota, has been shown to have important associations for a woman´s reproductive and general health. Currently, healthcare providers cannot offer comprehensive vaginal microbiome screening, but are limited to the detection of individual pathogens, such as high-risk human papillomavirus (hrHPV), the predominant cause of cervical cancer. There is no single test on the market that combines HPV, STI, and microbiome screening. Here, we describe a novel inclusive vaginal health assay that combines self-sampling with sequencing-based HPV detection and genotyping, vaginal microbiome analysis, and STI-associated pathogen detection. The assay includes genotyping and detection of 14 hrHPV types, 5 low-risk HPV types (lrHPV), as well as the relative abundance of 31 bacterial taxa of clinical importance, including Lactobacillus, Sneathia, Gardnerella, and 3 pathogens involved in STI, with high sensitivity, specificity, and reproducibility. For each of these taxa, reference ranges were determined in a group of 50 self-reported healthy women. The HPV sequencing portion of the test was evaluated against the digene High-Risk HPV HC2 DNA test. For hrHPV genotyping, agreement was 95.3% with a kappa of 0.804 (601 samples); after removal of samples in which the digene hrHPV probe showed cross-reactivity with lrHPV types, the sensitivity and specificity of the hrHPV genotyping assay were 94.5% and 96.6%, respectively, with a kappa of 0.841. For lrHPV genotyping, agreement was 93.9% with a kappa of 0.788 (148 samples), while sensitivity and specificity were 100% and 92.9%, respectively. This novel assay could be used to complement conventional cervical cancer screening, because its self-sampling format can expand access among women who would otherwise not participate, and because of its additional information about the composition of the vaginal microbiome and the presence of pathogens.Fil: Bik, Elisabeth M.. Ubiome;Fil: Bird, Sara W.. Ubiome;Fil: Bustamante, Juan Pablo. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; ArgentinaFil: Leon, Luis E.. Ubiome

Repurposing bioenergetic modulators against protozoan parasites responsible for tropical diseases

Malaria, leishmaniasis and trypanosomiasis are arthropod-borne, parasitic diseases that constitute a major global health problem. They are generally found in developing countries, where lack of access to preventive tools and treatment hinders their management. Because these parasites share an increased demand on glucose consumption with most cancer cells, six compounds used in anti-tumoral research were selected to be tested as antiparasitic agents in in vitro models of Leishmania infantum, Trypanosoma brucei, T. cruzi, and Plasmodium falciparum: dichloroacetic acid (DCA), 3-bromopyruvic acid (3BP), 2-deoxy-D-glucose (2DG), lonidamine (LND), metformin (MET), and sirolimus (SIR). No parasite-killing activity was found in L. infantum promastigotes, whereas DCA and 3BP reduced the burden of intra-macrophagic amastigotes. For T. brucei all selected compounds, but 2DG, decreased parasite survival. DCA, 2DG, LND and MET showed parasite-killing activity in T. cruzi. Finally, anti-plasmodial activity was found for DCA, 2DG, LND, MET and SIR. These results reinforce the hypothesis that drugs with proven efficacy in the treatment of cancer by interfering with ATP production, proliferation, and survival cell strategies might be useful in treating threatening parasitic diseases and provide new opportunities for their repurposing.info:eu-repo/semantics/publishedVersio

I Congreso internacional de la SECAH. Ex officina Hispana. Cádiz Mayo 2011

Acta del I Congreso Internacional de la Sociedad de Estudios de Cerámica antigua en Hispani

Two new glassfrogs (Centrolenidae: Hyalinobatrachium) from Ecuador, with comments on the endangered biodiversity of the Andes.

Background The Tropical Andes is the world's most biodiverse hotspot. This region contains >1,000 amphibian species, more than half of which are endemic. Herein we describe two new glassfrog species (Centrolenidae: Hyalinobatrachium) that we discovered within relatively unexplored and isolated localities of the Ecuadorian Andes. Methods We employed morphological, acoustic, and molecular methods to test the hypothesis that Hyalinobatrachium mashpi sp. nov and H. nouns sp. nov. are species new to science. Following standard methods, we generated mitochondrial sequences (16S) of 37 individuals in the genus Hyalinobatrachium. We inferred the phylogenetic relationships of the two new species in comparison to all other glassfrogs using Maximum Likelihood. In addition to describing the call of H. mashpi sp. nov., we performed a discriminant analysis of principal components (DAPC) with the advertisement call characteristics of several congeners. Results Based on an integrative taxonomy approach, we describe two new species. Morphological traits and the inferred phylogeny unambiguously place the new taxa in the genus Hyalinobatrachium. Both species are distinguished from other glassfrogs mainly by their dorsal coloration (i.e., dorsum lime green with small light yellow spots, head usually with interorbital bar) and transparent pericardium (i.e., the heart is visible through the ventral skin). The new species exhibit a high morphological similarity (i.e., cryptic) and occur within relatively close geographical proximity (closest aerial distance = 18.9 km); however, their uncorrected p distance for the mitochondrial gene 16S is 4.6-4.7%, a value that greatly exceeds the genetic distance between closely related species of centrolenid frogs. The DAPC revealed that the advertisement call of H. mashpi sp. nov. is acoustically distinct. Discussion Our findings are congruent with several previous studies that report a high degree of endemism in the Toisán mountain range, which appears to be isolated from the main Andean cordillera for some amphibian groups. We recommend that both H. mashpi sp. nov. and H. nouns sp. nov. be listed as Endangered, following IUCN criteria. These new species provide another example of cryptic diversity in the Andes-further evidence that the region fosters much more biodiversity than we have the resources to catalog. Threatened by mining and other exploitative industries, these glassfrogs and many other yet-to-be-discovered Andean species highlight the dire need for effective conservation measures-especially in northwestern Ecuador

Circulating cell-free DNA is a predictor of short-term neurological outcome in stroke patients treated with intravenous thrombolysis

Altres ajuts: This work has been funded by Instituto de Salud Carlos III, grant FIS PI15/354, co-financed by the European Regional Development Fund (FEDER). AB is supported by a Rio Hortega contract CM13/00265 from the Instituto de Salud Carlos III.Circulating cell-free DNA (cfDNA) has been described as a prognostic marker for several diseases. Its prognostic value for short-term outcome in stroke patients treated with intravenous thrombolysis remains unexplored. cfDNA was measured on admission in 54 tissue plasminogen activator (tPA)-treated patients and 15 healthy controls using a real-time quantitative polymerase chain reaction assay. Neurological outcome was assessed at 48 h. Predictors of neurological improvement were evaluated by logistic regression analysis, and the additional predictive value of cfDNA over clinical variables was determined by integrated discrimination improvement (IDI). Stroke patients presented higher baseline cfDNA than healthy controls (408.5 (179-700.5) vs. 153.5 (66.9-700.5) kilogenome-equivalents/L, p = 0.123). A trend towards lower cfDNA levels was found in patients who neurologically improved at 48 h (269.5 (143.3-680) vs. 504 (345.9-792.3) kilogenome-equivalents/L, p = 0.130). In logistic regression analysis, recanalization at 1 h and cfDNA < 302.75 kilogenome-equivalents/L was independently associated with neurological improvement after adjustment by age, gender and baseline National Institutes of Health Stroke Scale score. The addition of cfDNA to the clinical predictive model improved its discrimination (IDI = 21.2% (9.2-33.3%), p = 0.009). These data suggest that cfDNA could be a surrogate marker for monitoring tPA efficacy by the prediction of short-term neurological outcome

Instalación de recubrimientos cerámicos para pisos

Presenta una amplia visión de los productos cerámicos y de los sistemas de instalación para diferentes ambientes, y explica la instalación de baldosas cerámicas sobre pisos.It presents a broad vision of ceramic products and installation systems for different environments, and explains the installation of ceramic tiles on floors.La baldosa cerámica -- El pavimento cerámico con relación al uso -- La baldosa cerámica para pavimentos -- Ambientes -- Matemáticas básicas aplicadas -- Instalación de enchapes para pisos -- Sistemas de corte y perforación -- Lista de comprobación del residente o maestro de obra.naConvenio Ascolceramistas - SENA71 página

Post-fledging dependence period and onset of natal dispersal in Bearded Vultures (Gypaetus barbatus): new insights from GPS satellite telemetry.

Utilizamos telemetría satelital para estudiar el periodo de dependencia post emplumamiento de tres individuos silvestres y seis individuos cautivos de Gypaetus barbatus. El primer vuelo tuvo lugar cuando los pollos tenían 126 ± 7 días de edad (rango  =  111-134 días) y el inicio de la dispersión ocurrió a los 193 ± 40 días desde el primer vuelo (rango  =  143-231 días). Los jóvenes permanecieron en sus áreas natales (o de liberación, en el caso de aves criadas en cautiverio) durante los primeros dos meses desde la fecha de emplumamiento. Luego, comenzaron a incrementar progresivamente la distancia a sus áreas natales; durante este tiempo, la mayoría de los desplazamientos de larga distancia (>15 km) tuvieron lugar antes del mediodía. Los tamaños de las áreas de acción registrados en este estudio fueron superiores a los descritos con anterioridad para la especie, principalmente debido a la diferencia en los métodos de rastreo empleados: la telemetría satelital permitió la detección de movimientos de larga distancia más allá del rango de medición del rastreo por radio convencional. Finalmente, la adquisición de la independencia (i.e., emancipación) se solapó con la siguiente temporada de cría de los adultos

Evaluation of macroscopic lesions of different lung lobes in swine using a percentage classification model

Las enfermedades respiratorias en porcinos tienen un gran impacto económico, considerándose uno de los principales problemas en la producción intensiva porcina. El objetivo de este estudio fue evaluar macroscópicamente los diferentes lóbulos pulmonares en una planta de beneficio del centro-occidente de Risaralda, usando un modelo de calificación porcentual. Mediante un estudio descriptivo, se evaluaron 358 pulmones durante la fase de eviscerado, provenientes de 9 lotes de diferentes granjas; se recolectaron datos porcentuales según el grado de lesión macroscópica observada para cada uno de los lóbulos pulmonares. Con estos datos se calcularon los promedios para cada uno de los lóbulos y se compararon entre el total de muestras observadas y para cada uno de los lotes. El análisis de las observaciones macroscópicas permitió establecer una prevalencia de lesiones pulmonares del 85% (305/358), mientras que el 15% (53/358) no evidenciaron ningún tipo de lesión. El 13% (43/358) presentaron lesiones indicativas de pleuritis y el 71% (253/358) de cicatrización. La comparación de los promedios de lesión entre los diferentes lóbulos pulmonares mostró que el más afectado fue el cardiaco derecho, con un valor promedio de 2,1%, mientras que el menos afectado fue el accesorio con un valor promedio de 0,97%. En este estudio se encontró que los lóbulos cardiacos derecho e izquierdo fueron los más afectados del total de pulmones evaluados, estos resultados se pueden relacionar con los signos respiratorios, el estado sanitario y los parámetros productivos de los diferentes lotes en la granja, lo que contribuye a establecer, junto con otras pruebas complementarias un diagnóstico presuntivo en el caso de afecciones respiratorias. Mostrando que el método de evaluación porcentual podría ser una herramienta rápida y eficaz de estudio de lesiones postmorten en plantas de beneficio.Respiratory diseases in pigs have a great economic impact, considering it is one of the main problems in intensive swine production. The objective of this study was to macroscopically evaluate the different pulmonary lobes in a processing plant located center-west of Risaralda, using a percentage qualification model. Through a descriptive study, 358 lungs were evaluated during the evisceration phase, the specimens came from 9 lots of different farms. Data was collected according to the degree of macroscopic lesion observed for each of the pulmonary lobes in percentages. With this data, averages were taken for each of the lobes and compared between the total of samples observed and for each of the lots. In addition, more specific lesions were evaluated on a scale of 0-5 as indicators of pleurisy and scarring. The analysis of the macroscopic observations showed that the majority of the lungs, presented some degree of lesion with an 85% (305/358) while only 15% (53/358) showed no type of lesion. 13% (43/358) presented lesions indicative of pleurisy and 71% (253/358) showed scarring. The comparison of the lesion averages between the different pulmonary lobes showed that the most affected was the right cardiac lobe with an average value of 2.10% while the least affected was the accessory lobe with an average value of 0.97%. In this study it was found that the right and left cardiac lobes were the most affected out of the totality of lungs evaluated, these results can be related to the respiratory signs, the health status and the productive parameters of the different batches in the farm, helping to determine a presumptive or final etiological diagnosis. This shows that the percentage evaluation method could be a quick and effective tool for the study of postmortem lesions in processing plants

Evidencias arqueológicas de desplomes paramentales traumáticos en las Termas Marítimas de Baelo Claudia. Reflexiones arqueosismológicas

Durante los años 2011 a 2013 se han localizado, identificado y excavado parcialmente los restos de un nuevo complejo termal en la ciudad hispanorromana de Baelo Claudia (Tarifa, Cádiz), situado en el suburbium occidental de la ciudad, junto a la línea de costa. Denominadas Termas Marítimas, construidas en la primera mitad del s. II d.C. y abandonadas en época de Diocleciano/Constantino han sido excavadas estratigráficamente con detalle, habiéndose detectado la existencia de fases anteriores (que se remontan al s. II a.C.) y una continuidad de uso del ambiente en época tardorromana y moderna, vinculada con la explotación de los recursos marinos. Especialmente singular ha sido la constatación del desplome traumático de parte de los paramentos de las habitaciones del edificio en dos momentos concretos: por un lado en la Antigüedad Tardía (500 d.C. circa), ya que una unidad muraria de una de las habitaciones (H-3), anexa a la natatio, se localizó completamente derrumbada sobre el suelo, conexionada; y por otro, el desplome del muro oeste de la natatio y el oriental de la cisterna, estructuras de más de seis metros de longitud y cuatro de altura mínima conservada, desplomadas sobre los niveles de abandono del asentamiento en época bajomedieval o moderna (ss. XIV-XV d.C.). Se trata en ambas ocasiones de colapsos estructurales no habituales en circunstancias normales en los procesos de sedimentación arqueológica, por lo que es muy probable que su desplome se pueda vincular con eventos sísmicos u otras causas naturales similare