2 research outputs found

    Cultivo in vitro de un híbrido comercial de Thymus piperella L. (Lamiaceae)

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    Las especies del género Thymus L. (Lamiaceae) presentan un gran interés como plantas medicinales, condimentarías y de extractos, con múltiples aplicaciones actuales y potenciales debido a la alta riqueza en metabolitos secundarios bioactivos que producen. Concretamente, especies como Thymus piperella L., prácticamente endémica de la Comunidad Valenciana, tienen un amplio abanico de usos tradicionales en este sentido. Aunque se ha estudiado la micropropagación de esta especie y se conoce la composición en compuestos volátiles en tres quimiotipos silvestres descritos dentro de su área de distribución, todavía no se ha estudiado si las condiciones de cultivo in vitro pueden afectar a la producción de estos metabolitos en esta especie o en sus híbridos comerciales de uso tradicional. Por ello, el principal objetivo de este trabajo fue desarrollar un protocolo de micropropagación de un híbrido comercial de uso popular de Th. piperella. Los resultados obtenidos mostraron una buena capacidad de adaptación e inicio del cultivo in vitro tras los dos tratamientos de esterilización aplicados. Además, los distintos parámetros de desarrollo vegetal medidos en la fase de multiplicación mostraron valores significativamente mayores en medio Murashige y Skoog (MS) suplementado con 0,013 mg/l de 2-isopenteniladenina (2iP), o sin reguladores del crecimiento, y 30 g/l de sacarosa y solidificado con 5,5 g/l de Plant Agar tras compararlos con los otros tratamientos. Finalmente, la prueba realizada acerca del contenido en compuestos volátiles mediante SPME-GC/MS-HS reveló que se produjo un aumento destacable de timol en las plantas cultivadas in vitro en comparación con las muestras procedentes de planta silvestre. Por todo ello, los resultados obtenidos en el presente trabajo podrían suponer la base para el desarrollo de futuros estudios acerca de la elicitación de este compuesto mediante sistemas de cultivo in vitro con material vegetal de Th. piperella o de sus híbridos comerciales.Species of the genus Thymus L. (Lamiaceae) are of great interest as medicinal plants, seasoning and extracts with multiple current and potential applications due to the high richness of the bioactive secondary metabolites they produce. Specifically, species such as Th. piperella L., practically endemic to the Valencian Community, have a wide range of traditional uses in this sense. Although the micropropagation of this species has been studied and the composition of volatile compounds in three chemotypes described within its distribution area is known, it has not yet been studied how in vitro culture conditions can affect the production of these metabolites in Th. piperella or in its commercial hybrids of traditional use. Therefore, the main objective of this work was to develop a micropropagation protocol of a popularly used commercial hybrid of Th. piperella. The results obtained showed a good capacity of adaptation and initiation of in vitro culture after the two sterilization treatments applied. In addition, the different plant development parameters measured in the multiplication phase showed significantly higher values in Murashige and Skoog (MS) medium supplemented with 0.013 mg/l 2-isopentenyladenine (2iP), or without plant growth regulators, plus 30 g/l sucrose and solidified with 5.5 g/l Plant Agar when compared to the other treatments. Finally, the test performed on the content of volatile compounds by SPME-GC/MS-HS revealed that there was a remarkable increase of thymol in the in vitro cultivated plants compared to the samples from wild plant. Therefore, the results obtained in the present work could be the basis for the development of future studies on the elicitation of this compound by in vitro culture systems with plant material of Th. piperella or its commercial hybrids.Biotecnologí

    In vitro propagation and phytochemistry of thymol-producing plants from a horticultural form of Thymus x josephi-angeli Mansanet & Aguil. (Lamiaceae)

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    Thymus L. is of great interest in horticulture as ornamentals, spices, and medicinal plants, as well as in the extracts industry due to the richness in bioactive specialized metabolites. The natural hybrid T. x josephi-angeli Mansanet & Aguil. is produced in Spain, as its horticultural forms are very popular for domestic uses and gardening. However, its micropropagation and chemical composition have not been studied yet. Therefore, the main objective of this work was to develop a micropropagation procedure for T. x josephi-angeli, and to check whether the in vitro culture had an impact on the chemical profile of the plants. The results showed a high initiation rate (>91%) after two sterilization treatments were applied. Moreover, a micropropagation rate of around 21 new rooted explants per culture cycle was obtained in treatment M7 (Murashige and Skoog with 0.064 uM 6-(gamma,gamma-Dimethylallylamino)purine) when compared to the other 10 treatments performed. Acclimatization was successful in all three approaches tested (>75%), and all plants kept growing after 4 months of outdoor cultivation. Finally, 36 volatiles were identified, and the content of major compounds remained not statistically different in acclimatized plants when compared to the wild-type plants according to the analyses made by HS-SPME-GC/MS and SPME-GC/MS. This chemical stability points out the uniformity of the microplants and the suitability of the procedure applied in this study for T. xjosephi-angeli horticultural production using in vitro techniques
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