Proteome Comparison Between Natural Desiccation-Tolerant Plants and Drought-Protected Caspicum annuum Plants by Microbacterium sp. 3J1

Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for improving drought tolerance in crops. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study, we supplemented drought-tolerant and drought-sensitive plant rhizospheres with Microbacterium sp. 3J1 and analyzed their proteomes under drought to investigate the plant-microbe interaction. We also compare this root proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of Microbacterium sp. 3J1 subjected to drought to analyze its contribution to the plantmicrobe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.Spanish Ministry for Economy and CompetitivenessEuropean Union (EU) P11-RNM-7844 P18-RT-976 CTM2017-84332-R CGL2017-91737-EXPAndalusian Regional GovernmentSpanish Ministry of Econom

Cepa bacteriana CECT624, usos y producto xeroprotector producido por la misma

Número de publicación: ES2362035 B2. Número de solicitud: 200931119.Cepa bacteriana CECT7624, usos y producto xeroprotector producido por la misma.Microorganismo de la especie bacteriana Microbacterium sp. con número de acceso CECT7624. La presente invención también se refiere al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha planta. Además, la presente invención se refiere al uso de dicho microorganismo o de una población del mismo para la producción de una composición xeroprotectora, donde dicha composición comprende trehalosa, ácido oxoglucurónico, lactato, glutamato, glutamina y piruvato, o además comprende fucosa. La presente invención también se refiere al método para aumentar la tolerancia al estrés hídrico de una planta o para producir la composición xeroproctectora de la presente invención.Universidad de Granad

Plant Drought Tolerance Enhancement by Trehalose Production of Desiccation-Tolerant Microorganisms

A collection of desiccation-tolerant xeroprotectant-producing microorganisms was screened for their ability to protect plants against drought, and their role as plant growth-promoting rhizobacteria was investigated in two different crops (tomato and pepper). The most commonly described biochemical mechanisms for plant protection against drought by microorganisms including the production of phytohormones, antioxidants and xeroprotectants were analyzed. In particular, the degree of plant protection against drought provided by these microorganisms was characterized. After studying the findings and comparing them with results of the closest taxonomic relatives at the species and strain levels, we propose that trehalose produced by these microorganisms is correlated with their ability to protect plants against drought. This proposal is based on the increased protection of plants against drought by the desiccation-sensitive microorganism Pseudomonas putida KT2440, which expresses the otsAB genes for trehalose biosynthesis in trans

Cepa bacteriana CECT626, usos y producto xeroprotector producido por la misma

Número de publicación: ES2361316 B1. Número de solicitud: 200931118.Microorganismo de la especie bacteriana Arthrobacter sp. con número de acceso CECT7626. La presente invención también se refier al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha planta. Además, la presente invención se refiere al uso de dicho microorganismo o de una población del mismo par la producción de una composición xeroprotectora, donde dicha composición comprende glucosa, acetato, lactato y valina. Y, la presente invención también se refiere al método para aumentar la tolerancia al estrés hídrico de una planta o para producir la composición xeroprotectora de la presente invención.The invention relates to a microorganism of the Arthrobacter sp. bacterial species (access number CECT7626). The invention also relates to the use of said microorganism or a population of same in order to enhance the water stress tolerance of a plant or to enhance the growth of a plant with said plant under optimum water content conditions. The invention further relates to the use of said microorganism or a population of same for the production of a xeroprotectant composition, said composition comprising glucose, acetate, lactate and valine. Furthermore, the invention relates to a method for enhancing the water stress tolerance of a plant or producing the xeroprotectant composition.Universidad de Granad