Simple expressions for performance parameters of complex filters, with applications to super-Gaussian phase filters

To study the three-dimensional (3-D) behavior produced by complex filters, we have extended the expressions for the axial and the transverse gain to the case in which the best image plane is not near the paraxial focus. Super-Gaussian phase filters are proposed to control the 3-D image response of an optical system. Super-Gaussian phase filters depend on several parameters that modify the shape of the phase filter, producing tunable control of the 3-D response of the optical system. The filters are capable of producing a wide range of optical effects: transverse superresolution with high depth of focus, 3-D superresolution, and transverse apodization with different axial responses

Ehmi: Review and guidelines for deployment on autonomous vehicles

Human-machine interaction is an active area of research due to the rapid development of autonomous systems and the need for communication. This review provides further insight into the specific issue of the information flow between pedestrians and automated vehicles by evaluating recent advances in external human-machine interfaces (eHMI), which enable the transmission of state and intent information from the vehicle to the rest of the traffic participants. Recent developments will be explored and studies analyzing their effectiveness based on pedestrian feedback data will be presented and contextualized. As a result, we aim to draw a broad perspective on the current status and recent techniques for eHMI and some guidelines that will encourage future research and development of these systems

En los límites de la difracción: superresolución y apodización en sistemas ópticos. Implementación en moduladores LCD

La difracción es el fenómeno que ocurre cuando las ondas se propagan y se encuentran con un obstáculo. Se trata de un aspecto fundamental en Óptica, ya que todos los sistemas ópticos están afectados por la difracción, produciendo en general dos efectos desfavorables. Por un lado produce un ensanchamiento de los haces a lo largo de su propagación. Por otro lado, limita el tamaño mínimo posible del haz al ser enfocado. A lo largo de los años, ha habido multitud de intentos de mejorar o reducir estos inconvenientes tanto en la difracción de campo lejano como también en la de campo cercano. En este artículo nos centraremos en el estudio de los límites impuestos por la difracción de campo lejano, y especialmente en el segundo problema que explicábamos antes: cómo mejorar el poder resolutivo o de una manera más general, la respuesta tridimensional de un sistema, ya que nosotros estamos especialmente interesados en sistemas ópticos como formadores de imagen. En segundo lugar mostraremos el uso de los moduladores espaciales de luz basados en cristal líquido (LC-SLM) para crear filtros de transmisión y/o fase no uniforme y también la generación de elementos ópticos difractivos orientados a formación de imagen. Mostraremos resultados experimentales que cubren un amplio abanico de posibilidades ofrecidas por el uso conjunto de los filtros mencionados y de los dispositivos LC-SLM

Programmable axial apodizing and hyperresolving amplitude filters with a liquid-crystal spatial light modulator

Amplitude-transmitting filters for apodizing and hyperresolving applications can be easily implemented by use of a two-dimensional programmable liquid-crystal spatial light modulator operating in a transmission-only mode. Experimental results are in excellent agreement with theoretical predictions. This approach can permit the analysis of various filter designs and can allow the filters to be changed rapidly to modify the response of an optical system

Polarimetric imaging microscopy for advanced inspection of vegetal tissues

Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques

Retrieving physical information of depolarizing systems

Light interaction with material systems may introduce depolarization to the incident light. This phenomenon comes from multiple scattering processes that take place inside the media and strongly depends on the particle characteristics. In the case of botany, plant leaves can be understood as depolarizing systems. A non-contact method to analyze these samples consist of illuminating them with well-known polarized light and study the scattered light to retrieve the physical characteristics of the sample. This physical study can be done by measuring the Mueller matrix of samples, in which the physical information of samples is encoded in their 16 elements and further mathematical treatment is required to extract the information. In the case of scattering systems, the depolarization content carries very valuable information but it is usually not inspected in the botanic field. A way to study depolarized content is by determining the so-called depolarization index P¿, which gives an overall measure of the degree of depolarization of a system but it does not measure possible anisotropic dependence of the depolarization. For instance, a depolarizer equally depolarizing any fully polarized input polarization or a depolarizer that depolarizes them in a strongly heterogenous way, may lead to the same P¿ value. In contrast, the Indices of Polarimetric Purity (IPP) are a group of metrics that further synthesize the depolarizing content, taking into account the anisotropic depolarization. In this work, we describe the main physical characteristics of samples achieved by using these IPP through plant samples. Moreover, we show how IPP highlights some structures hidden in regular intensity measurements, highlighting the potential of these metrics for botanical applications

Polarization gating based on Mueller matrices

We present mathematical formulas generalizing polarization gating (PG) techniques. PG refers to a collection of imaging methods based on the combination of different controlled polarization channels. In particular, we show how using the measured Mueller matrix (MM) of a sample, a widespread number of PG configurations can be evaluated just from analytical expressions based on the MM coefficients. We also show the interest of controlling the helicity of the states of polarization used for PG-based metrology, as this parameter has an impact in the image contrast of samples. In addition, we highlight the interest of combining PG techniques with tools of data analysis related to the MM formalism, such as the well-known MM decompositions. The method discussed in this work is illustrated with the results of polarimetric measurements done on artificial phantoms and real ex-vivo tissues

The orosomucoid 1 protein (α1 acid glycoprotein) is overexpressed in odontogenic myxoma

Odontogenic myxoma (OM) is a benign, but locally invasive, neoplasm occurring in the jaws. However, the molecules implicated in its development are unknown. OM as well as Dental Follicle (DF), an odontogenic tissue surrounding the enamel organ, is derived from ectomesenchymal/mesencyhmal elements. To identify some protein that could participate in the development of this neoplasm, total proteins from OM were separated by two-dimensional electrophoresis and the profiles were compared with those obtained from DF, used as a control

Indices of polarimetric purity: application in biological tissues

Complete characterization of biological samples is of potential interest in different industrial and research areas, as for instance, in biomedical applications, for the recognition of organic structures or for the early detection of some diseases. During the last decades, polarimetric methods are experiencing an increase of attention in the study of biomedical tissues, and they are nowadays used in such framework to provide qualitative (polarimetric imaging) and quantitative (data processing) information for the studied samples. Polarimetric methods are based on the analysis of polarization modifications produced by light-matter interactions which can be triggered by a number of complex internal processes but can be roughly understood as the result of the combination of three pure polarimetric features of the sample: its diattenuation, retardance and depolarization. For the analysis of the depolarization content, we propose the use of the Indices of Polarimetric Purity (IPP) to describe the sample behavior. Related with the randomness of the scattering processes, IPPs provide more information of depolarizing systems than the widely used depolarization index (p^), which further synthetize the depolarization content of samples. Moreover, certain combinations of IPP parameters leads to p^. As a result, IPPs allow the revelation of some structures from tissue samples hidden in regular intensity images of even in the p^ channel, leading to better tissue classification results. In this work, we present different applications of IPPs in biomedical tissue that show its potential, which are not restricted to the biomedical framework as relevant results in plants characterization are also presented.Peer reviewe

Polarimetric imaging of biological tissues based on the indices of polarimetric purity

We highlight the interest of using the indices of polarimetric purity (IPPs) to the inspection of biological tissues. The IPPs were recently proposed in the literature and they result in a further synthetization of the depolarizing properties of samples. Compared with standard polarimetric images of biological samples, IPP-based images lead to larger image contrast of some biological structures and to a further physical interpretation of the depolarizing mechanisms inherent to the samples. In addition, unlike other methods, their calculation do not require advanced algebraic operations (as is the case of polar decompositions), and they result in 3 indicators of easy implementation. We also propose a pseudo-colored encoding of the IPP information that leads to an improved visualization of samples. This last technique opens the possibility of tailored adjustment of tissues contrast by using customized pseudo-colored images. The potential of the IPP approach is experimentally highlighted along the manuscript by studying 3 different ex-vivo samples. A significant image contrast enhancement is obtained by using the IPP-based methods, compared to standard polarimetric images