CRL Ubiquitin Ligases and DNA Damage Response

Cullin/RING ubiquitin ligases (CRL) comprise the largest subfamily of ubiquitin ligases. CRLs are involved in cell cycle regulation, DNA replication, DNA damage response (DDR), development, immune response, transcriptional regulation, circadian rhythm, viral infection, and protein quality control. One of the main functions of CRLs is to regulate the DDR, a fundamental signaling cascade that maintains genome integrity. In this review, we will discuss the regulation of CRL ubiquitin ligases and their roles in control of the DDR

The Context of Cloud Computing/Services Adoption in Business: A Systematic Review with Activity Theory perspective

Cloud computing has been established as a prominent research topic with the rise of a ubiquitous provision of computing resources over the last decade. According to literature review, previous studies are found focusing on the technical issue mostly; however, accompanying with the cloud service developing progress, there are a wide range of applications for adopting cloud computing/services and without an overall comprehension. This study aims to proposed a conceptual framework to systematically explore the activities and elements related to cloud computing/services adoption in business. The research framework is found useful for understanding the context of cloud computing and services and the preliminary findings are helpful to further explore the related activities and relationships behind on cloud computing/services adoption in business

Development and validation of the short-form Adolescent Health Promotion Scale.

BACKGROUND: Health-promoting lifestyle choices of adolescents are closely related to current and subsequent health status. However, parsimonious yet reliable and valid screening tools are scarce. The original 40-item adolescent health promotion (AHP) scale was developed by our research team and has been applied to measure adolescent health-promoting behaviors worldwide. The aim of our study was to examine the psychometric properties of a newly developed short-form version of the AHP (AHP-SF) including tests of its reliability and validity. METHODS: The study was conducted in nine middle and high schools in southern Taiwan. Participants were 814 adolescents randomly divided into two subgroups with equal size and homogeneity of baseline characteristics. The first subsample (calibration sample) was used to modify and shorten the factorial model while the second subsample (validation sample) was utilized to validate the result obtained from the first one. The psychometric testing of the AHP-SF included internal reliability of McDonald's omega and Cronbach's alpha, convergent validity, discriminant validity, and construct validity with confirmatory factor analysis (CFA). RESULTS: The results of the CFA supported a six-factor model and 21 items were retained in the AHP-SF with acceptable model fit. For the discriminant validity test, results indicated that adolescents with lower AHP-SF scores were more likely to be overweight or obese, skip breakfast, and spend more time watching TV and playing computer games. The AHP-SF also showed excellent internal consistency with a McDonald's omega of 0.904 (Cronbach's alpha 0.905) in the calibration group. CONCLUSION: The current findings suggest that the AHP-SF is a valid and reliable instrument for the evaluation of adolescent health-promoting behaviors. Primary health care providers and clinicians can use the AHP-SF to assess these behaviors and evaluate the outcome of health promotion programs in the adolescent population

Methanotrophic abundance and community fingerprint in pine and tea plantation soils as revealed by molecular methods

Understanding the community structure of methane-oxidizing bacteria (methanotrophs) is important to assess the microbial oxidation of the greenhouse gas methane (CH4) in soil under different land uses. Soil samples were collected from two plantation plots of pine and tea in southern China. Methanotrophic abundance was quantified with quantitative real-time polymerase chain reaction (qPCR) based on the 16S rRNA and pmoA genes, and the community fingerprint was characterized with denaturing gradient gel electrophoresis (DGGE) targeting the pmoA gene. No significant difference in the gene copy numbers of methanotrophs was found between the pine and tea land-use, regardless of 16S rRNA and pmoA genes. Higher abundance of type I (1.35 vs 1.66×108 copie g-1 soil) over type II methanotrophs (8.59 vs 10.9 × 107) were found both in pine and tea plantation soils. Apparent differences in methanotrophic community fingerprint were observed between the pine and tea treatments. Correlations analysis between methanotrophic abundance and soil characteristics, combining with canonial correspondence analysis (CCA) regarding community fingerprint and environmental parameters indicated that soil pH and available phosphorus were the most important factors potentially affecting the methanotrophic community diversity in the acidic red soil.Key words: Denaturing gradient gel electrophoresis (DGGE), land use, methanotrophs, pmoA gene, quantitative real-time PCR (qPCR)

A review of ammonia-oxidizing bacteria and archaea in Chinese soils

Ammonia (NH3) oxidation, the first and rate-limiting step of nitrification, is a key step in the global Nitrogen (N) cycle. Major advances have been made in recent years in our knowledge and understanding of the microbial communities involved ammonia oxidation in a wide range of habitats, including Chinese agricultural soils. In this mini-review, we focus our attention on the distribution and community diversity of ammonia-oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) in Chinese soils with variable soil properties and soil management practices. The niche differentiation of AOB and AOA in contrasting soils have been functionally demonstrated using DNA-SIP (stable isotope probing) methods, which have shown that AOA dominate nitrification processes in acidic soils, while AOB dominated in neutral, alkaline and N-rich soils. Finally, we discuss the composition and activity of ammonia oxidizer in paddy soils, as well as the mitigation of the greenhouse gas nitrous oxide (N2O) emissions and nitrate leaching via inhibition of nitrification by both AOB and AOA

AOB Nitrosospira cluster 3a.2 (D11) dominates N2O emissions in fertilised agricultural soils.

CRediT authorship contribution statement Na Deng: Writing – review & editing, Methodology, Investigation, Data curation. Cecile Gubry-Rangin: Writing – review & editing, Methodology, Conceptualization. Xiao-Tong Song: Writing – review & editing, Methodology, Data curation. Xiao-Tang Ju: Writing – review & editing, Conceptualization. Si-Yi Liu: Methodology, Data curation. Ju-Pei Shen: Writing – review & editing, Data curation. Hong-jie Di: Writing – review & editing. Li-Li Han: Writing – review & editing, Methodology. Li-Mei Zhang: Writing – review & editing, Methodology, Data curation, Conceptualization.Peer reviewe