General circulation model simulations of Southern African regional climate.

Dissertation submitted to the Faculty of Science, University of the Witwatersraild, Johannesburg for the Degree of Master of Science.Six general circulation model simulations of present-day southern African climate are assessed, Each of these models are early-generation equilibrium climate models linked to simple mixed-slab oceans. Simulations of surface air temperature over the subcontinent are sensitive to the grid-scale parameterisation of convection in summer. At high latitudes, large simulation errors are caused by errors in the specification of sea-ice albedo feedbacks. Increased spatial resolution and the inclusion of a gravity wave drag term in the momentum equations results in a markedly-improved simulated mean sea level pressure distribution. Tho models successfully simulate the pattern of rainfall seasonality over the Subcontinent, although grid-point simulation of precipitation is unreliable. Treatment of convection, cloud radiative feedbacks and the oceans by this generation of models is simplistic, and consequently there is a large degree of uncertainty associated with predictions of future climate under doubled-carbon dioxide conditions. For this reason, more reliable estimates of future conditions will be achieved using only those models which reproduce present climate most accurately. Early-generation general circulation models suggest a warming of 4°C to 5°C for the southern African region as a whole throughout the year. Over the subcontinent, warming is expected to be least in the tropics, and greatest in the dry subtropical regions in winter. Estimated changes in mean sea level pressure indicate a southward shlft of all pressure systems, with a weakening of the subtropical high pressure belt and mid-latitude westerlies. Little agreement exists between the models concerning predictions of regional precipitation change. However, broad scale changes in precipitation patterns are in accordance with predicted circulation changes over the subcontinent. Generally wetter conditions may be expected in the tropics throughout the year and over the summer rainfall region during summer. Decreased winter rainfall may be expected over the winter rainfall region of the south-western Cape. However, estimated precipitation changes are grid-point specific and therefore must riot be over-interpreted. The present climate validation has resulted in more reliable estimates of future conditions for the southern African region. This approach should be extended to recent slrnulations which include more comprehensive treatment of important physical processes.Andrew Chakane 201

Simulated changes in extreme rainfall over southern Africa

A general circulation model simulation is used to investigate possible changes in rainfall over southern Africa resulting from a doubling of atmospheric carbon dioxide. Simulated increases in rainfall intensity are found to be a spatially coherent and an apparently less regionally dependent signal of climatic change than changes in annual means or number of rain‐days. Accordingly, increases in both the frequency and intensity of extreme daily rainfall events are simulated throughout most of the subcontinent. Simulated increases in the intensity of the lowest frequency floods are shown to be particularly severe, suggesting that greenhouse‐related climatic change may be most detectable through an increase in extreme flood events rather than changes in long‐term means. Similar results are evident when changes in the frequency and intensity of prolonged rainfall events, measured over a period of five consecutive days, are analysed. All results are qualitatively similar to those for the Australian region, except that the model's sensitivity to sharp changes in topography over southern Africa is highlighted

Droughts over southern Africa in a doubled-CO2 climate

The southern African region is susceptible to climatic extremes and particularly to extended dry periods. Possible changes in the probability of dry years under doubled‐CO2 conditions are examined using output from the CSIRO nine‐level general circulation model. Changes in annual mean rainfall are not expected to be significant. However, the model simulates an increase in the probability of dry years in the tropics, to the south‐west of the subcontinent, as well as over the western and eastern parts of South Africa and southern Mozambique, where large percentage increases in the most intense dry spells are indicated. A decrease in the frequency of dry years is simulated over much of the interior of the subcontinent south of 10°S. In regions where the frequency of dry years decreases, the most severe events occur less often. The CSIRO nine‐level model indicates a shift in the frequency distribution of daily rainfall events under doubled‐CO2 conditions. A small change in the frequency distribution of daily rainfall events may have further implications for the frequency of mid‐summer droughts during the peak summer rainfall period of December–February. Increases in the frequency of mid‐summer droughts are simulated over the eastern part of the subcontinent south of 20°S

Modelling present and future climates over Southern Africa.

Thesis submitted to the Faculty of Science, Department of Geography and Environmental Studies, University of the Witwatersrand, Johannesburg, in fulfillment of the requirements for the Degree of Doctor of PhilosophyThe representation of contemporary southern African climate by a wide range of general circulation models used in climate studies is evaluated. In addition, projections of regional climate change by the models are interpreted in terms of their present climate performance. Projections of regional climate change by two different types of climate models are considered. First, projections of the equilibrium response to an instantaneous doubling of atmospheric carbon dioxide using atmospheric models linked to simple mixed-layer oceans are assessed. Second, projections of the transient response to gradually-increasing anthropogenic forcing by fully-coupled ocean-atmosphere general circulation models are considered. All of the mixed-layer models considered have been developed since 1990 and are more recent and generally higher-resolution versions of the models considered previously for southern Africa. The improved resolution and model physics result in a general improvement in the representation of several features of circulation around southern Africa. Specifically, these include the meridional pressure gradient, the zonal wind profile, the intensity and seasonal location of the circumpolar trough and the subtropical anticyclones, as well as planetary wave structure at 500 hPa. Atmospheric models forced by observed sea-surface temperatures simulate the large-scale circulation adjustments around southern Africa known to accompany periods of above- and below-average rainfall over the subcontinent. Fully-coupled models simulate the observed features of intra- and intra- annual variability in mean sea-level pressure, although the simulated variability is weaker than observed. Summer rainfall totals throughout southern Africa are overestimated by all of the models, although the pattern of rainfall seasonality over the subcontinent as a whole is well-reproduced. The inclusion of sulphate aerosols in addition to greenhouse gases does not result in a statistically significant improvement in the simulation of contemporary temperature variability over southern Africa. Warming projected by fully-coupled models is smaller than projections by mixed-layer models due to the fact that the transient response of the fully-coupled system and not an equilibrium response of an atmospheric model linked to a mixed-layer ocean is simulated. The inclusion of sulphate aerosols results in a reduction in the magnitude and rate of warming over southern Africa. Projected changes in the diurnal temperature range are seasonally-dependent, with increases in summer and autumn and decreases in winter. Simulated changes in mean sea-level pressure are small but similar in magnitude to observed anomalies associated with extended wet and dry spells over the subcontinent. No change in rainfall seasonality over southern Africa is expected. Nonetheless, little confidence exists in projected changes in total rainfall. While both types of model simulate a 10-15% decrease in summer rainfall on average, projected changes are smaller than the simulation errors and little inter-model consensus in terms of the sign of projected changes exists. No change in the location or intensity of anticyclonic circulation and divergence at 700 hPa in winter is expected. While fully-coupled models provide a more comprehensive treatment of the global climate system and the process of climate change, there is no evidence to conclude that current fully-coupled models should be used to the exclusion of mixed-layer models when developing regional climate change scenarios for southern Africa.Andrew Chakane 201

UniLingua: intercambio lingüístico interuniversitario Complutense

UniLingua es un proyecto de intercambio lingüístico virtual cuya misión es poner en contacto a los alumnos de la Universidad Complutense de Madrid con alumnos de otras universidades extranjeras para que desarrollen sesiones de intercambio lingüístico en línea. En esta memoria, se resumen el desarrollo y los resultados de la cuarta edición del proyecto, llevada a cabo en el curso académico 2023-2024.Depto. de Lengua Española y Teoría de la LiteraturaFac. de FilologíaFALSEsubmittedAPC financiada por la UC

Guidelines for the use and interpretation of assays for monitoring autophagy

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field