Effects of testosterone and exercise training on bone microstructure of rats

Background and Aim: Male hypogonadism results from failure to produce physiological levels of testosterone. Testosterone in men is essential in masculine development, sperm production, and adult man’s health. Osteoporosis is one of the consequences of hypogonadism. Regular physical exercise and exogenous testosterone administration are frequently used to prevent or treat this condition. This study aimed to understand the effects of lifelong exercise training and testosterone levels (isolated and together) in the main bone structure parameters. Materials and Methods: A total of 24 rats were used and randomly divided into four groups: Control group (CG; n=6), exercised group (EG, n=6), testosterone group (TG, n=6), and testosterone EG (TEG, n=6). A micro-computed tomography equipment was used to evaluate 15 bone parameters. Results: Both factors (exercise training and testosterone) seem to improve the bone resistance and microstructure, although in different bone characteristics. Testosterone influenced trabecular structure parameters, namely, connectivity density, trabecular number, and trabecular space. The exercise promoted alterations in bone structure as well, although, in most cases, in different bone structure parameters as bone mineral density and medullar mineral density. Conclusion: Overall, exercise and testosterone therapy seems to have a synergistic contribution to the general bone structure and resistance. Further studies are warranted, comparing different individual factors, as gender, lifestyle, or testosterone protocols, to constantly improve the medical management of hypogonadism (and osteoporosis)

The first full study of heavy metal(loid)s in western-European hedgehogs (Erinaceus europaeus) from Portugal

The western-European hedgehog (Erinaceus europaeus) is an insectivore with a wide distribution in Portugal and a potential tool for biomonitoring relevant One Health hazards, including heavy metal(loid)s’ pollution. The aim of this study was to positively contribute to the current knowledge about the metal(loid) pollution in Portugal. Forty-six hedgehogs (from rescue centres; with known provenance) were necropsied. Sex, age category and weight were determined. Spines, liver and kidney were collected, and metalloid concentrations were determined by inductively coupled plasma mass spectrophotometry (ICP-MS). In general, results did not present alarming metal(loid) concentrations, with the exception of cadmium (Cd) (in the kidneys) and copper (Cu). Hedgehogs from Viana do Castelo and Viseu showed elevated concentrations of arsenic (As) and Castelo Branco presented concerning values of cadmium (Cd). Adult and heavier hedgehogs tended to present higher levels of metal(loid)s. Sex does not seem to significantly affect the metal(loid)s’ concentrations. Further analysis would be needed to prioritize areas with detail and allow the application of the necessary mitigation strategies.Open access funding provided by FCT|FCCN (b-on). This work was supported by National Funds by the Fundação para a Ciência e a Tecnologia (FCT) e and Ministério da Ciência e Tecnologia (MCT). CJB and PAO received funding from FCT—reference of the project: UIDB/04033/2020. FS and ACC also received funding from FCT—references of the projects: UIDB/CVT/00772/2020 and LA/P/0059/2020. CJB was supported by FCT due to the PhD scholarship 2021.04520.BD.CJB also thanks FCT/MCTES for the financial support to CiiEM (10.54499/UIDB/04585/2020). CP, PP and EFS received financial support (reference of the project: UID/GEO/04035/2020). TLM was supported by the projects UIDB/05937/2020 and UIDP/05937/2020 also funded by FCT