Izdvajanje bakterije Escherichia coli O157:H7 i njezin dokaz lančanom reakcijom polimerazom u crijevima filipinskih šišmiša.

It is currently reported that bats in the Philippines harbor bacterial organism (Salmonella spp.) with pathogenic potential. The paper describes the conventional isolation and polymerase chain reaction (PCR) assay for the detection of another bacterium, Escherichia coli, from a sample population of 56 apparently healthy bats collected from Laguna and Quezon City, Philippines. Nineteen of the samples were positive for E. coli using the conventional method of isolation, while PCR molecularly detected the bacteria in 15 samples. The presence of hemolysin among the isolates was not observed. The isolates were subjected to E. coli O157:H7 serotype detection using the latex agglutination test and another PCR assay specific for this serotype. The data revealed that none of the isolates was positive for E. coli O157:H7 using serological and molecular diagnostic methods, which indicates that bats from Laguna and Quezon City, Philippines were not carriers of the pathogenic strain, E. coli O157:H7. The study also presents the first local report of conventional isolation and molecular detection of E. coli from Philippine bats.Poznato je da šišmiši na Filipinima mogu biti nositelji potencijalno patogenih bakterija, npr. salmonela. U radu je opisano izdvajanje i dokaz lančanom reakcijom polimerazom bakterije Escherichia coli iz 56 naizgled zdravih šišmiša uhvaćenih na području Laguna i Quezon City na Filipinima. E. coli bila je izdvojena iz 19 uzoraka, dok je lančanom reakcijom polimerazom ona bila dokazana u 15 uzoraka. Izolati nisu tvorili hemolizu, a lateks aglutinacija i specifični PCR rabljeni su za dokaz serovara O157:H7 bakterije E. coli. Nijedan izolat nije pripadao serovaru O157:H7, na osnovi čega se može zaključiti da šišmiši na području Laguna i Quezon City na Filipinima nisu nositelji patogenog soja E. coli O157:H7. Istraživanje je ujedno prvi dokaz o izdvajanju i molekularnoj identifikaciji bakterije E. coli u šišmiša na Filipinima

Izdvajanje bakterije Escherichia coli O157:H7 i njezin dokaz lančanom reakcijom polimerazom u crijevima filipinskih šišmiša.

It is currently reported that bats in the Philippines harbor bacterial organism (Salmonella spp.) with pathogenic potential. The paper describes the conventional isolation and polymerase chain reaction (PCR) assay for the detection of another bacterium, Escherichia coli, from a sample population of 56 apparently healthy bats collected from Laguna and Quezon City, Philippines. Nineteen of the samples were positive for E. coli using the conventional method of isolation, while PCR molecularly detected the bacteria in 15 samples. The presence of hemolysin among the isolates was not observed. The isolates were subjected to E. coli O157:H7 serotype detection using the latex agglutination test and another PCR assay specific for this serotype. The data revealed that none of the isolates was positive for E. coli O157:H7 using serological and molecular diagnostic methods, which indicates that bats from Laguna and Quezon City, Philippines were not carriers of the pathogenic strain, E. coli O157:H7. The study also presents the first local report of conventional isolation and molecular detection of E. coli from Philippine bats.Poznato je da šišmiši na Filipinima mogu biti nositelji potencijalno patogenih bakterija, npr. salmonela. U radu je opisano izdvajanje i dokaz lančanom reakcijom polimerazom bakterije Escherichia coli iz 56 naizgled zdravih šišmiša uhvaćenih na području Laguna i Quezon City na Filipinima. E. coli bila je izdvojena iz 19 uzoraka, dok je lančanom reakcijom polimerazom ona bila dokazana u 15 uzoraka. Izolati nisu tvorili hemolizu, a lateks aglutinacija i specifični PCR rabljeni su za dokaz serovara O157:H7 bakterije E. coli. Nijedan izolat nije pripadao serovaru O157:H7, na osnovi čega se može zaključiti da šišmiši na području Laguna i Quezon City na Filipinima nisu nositelji patogenog soja E. coli O157:H7. Istraživanje je ujedno prvi dokaz o izdvajanju i molekularnoj identifikaciji bakterije E. coli u šišmiša na Filipinima

Bat Coronaviruses and Experimental Infection of Bats, the Philippines

Virus-infected fruit bats showed no signs of clinical infection

A troubled top of the marine food chain: Ecotoxicological relevance and consideration to stranded cetaceans in Philippine waters

Anthropogenic contaminants such as polychlorinated biphenyls (PCBs), dichlorodiphenyltricloethanes (DDTs), heavy metals, and polycyclic hydrocarbons (PAHs) are continually being detected in various tissues of marine mammals in other parts of the world. Data correlating pollutant residues with altered reproductive and development states, liver disease, endocrine system damage, and alarming growth in cancer cases are reported for these marine top-level predators. If exposure has already been long-term, this may be more deterrent to a population’s continued success than a single, high-impact event. In the Philippines, there are data reporting the presence of these xenobiotics in almost all environmental media (sediments, soils, waters, mussels, fish, squids, and shrimps). Through the process of biomagnification, cetaceans found stranded in Philippine waters are greatly at risk. Moreover, reports show that these stranded cetaceans are used for human consumption in the country. Given these data, the high frequency of stranding events in the country are currently being maximized in an ecotoxicological, biomarker and histopathological research targeting at least 30 cetaceans found stranded (live and dead) in our waters. Findings of this pioneering study will be used to (1) provide knowledge & understanding of current contamination levels in these cetaceans, which are at the top of the food web, found stranded in Philippine waters, (2) educate our locals of possible human health impacts of cetacean consumption, and (3) present scientific data which will initialize development of ecotoxicological protocols in the Philippines using not just stranded cetaceans but also other marine mammal species or other marine species in general

A troubled top of the marine food chain: Ecotoxicological relevance and consideration to stranded cetaceans in Philippine waters

Anthropogenic contaminants such as polychlorinated biphenyls (PCBs), dichlorodiphenyltricloethanes (DDTs), heavy metals, and polycyclic hydrocarbons (PAHs) are continually being detected in various tissues of marine mammals in other parts of the world. Data correlating pollutant residues with altered reproductive and development states, liver disease, endocrine system damage, and alarming growth in cancer cases are reported for these marine top-level predators. If exposure has already been long-term, this may be more deterrent to a population’s continued success than a single, high-impact event. In the Philippines, there are data reporting the presence of these xenobiotics in almost all environmental media (sediments, soils, waters, mussels, fish, squids, and shrimps). Through the process of biomagnification, cetaceans found stranded in Philippine waters are greatly at risk. Moreover, reports show that these stranded cetaceans are used for human consumption in the country. Given these data, the high frequency of stranding events in the country are currently being maximized in an ecotoxicological, biomarker and histopathological research targeting at least 30 cetaceans found stranded (live and dead) in our waters. Findings of this pioneering study will be used to (1) provide knowledge & understanding of current contamination levels in these cetaceans, which are at the top of the food web, found stranded in Philippine waters, (2) educate our locals of possible human health impacts of cetacean consumption, and (3) present scientific data which will initialize development of ecotoxicological protocols in the Philippines using not just stranded cetaceans but also other marine mammal species or other marine species in general