Biotechnology for the Environment, A Report on the Joint United States - European Union Celebration of a Decade of Environmental Biotechnology Exchange Activities for Early Career Scientists, Project ID: 0011751

The joint EU-US Task Force on Environmental Biotechnology held a workshop entitled, 'A Celebration of a Decade of Environmental Biotechnology Exchange Activities' on October 17, 2005 in Brussels, Belgium. This was a fitting venue since Brussels was where the EU-US transatlantic initiative originated. The workshop brought together former trainees who are currently active in the field of environmental biotechnology in order to (1) assess the impact of the past training activities; (2) to promote further collaborations; and (3) to highlight working group and task force activities in this field. Presentations by the early career scientists filled the meeting day (see Appendix I and II for meeting agenda and abstract book, respectively). Task Force members chaired the various sessions. An additional poster session provided an opportunity for more intensive scientific exchange. The day culminated with a formal dinner and gathering of all participants. Agencies supporting the activities included DOE, USDA and NSF. Funds received from the DOE were exhausted and USDA and NSF allowed the Task Force to use unexpended monies (via no cost extensions) to facilitate future fellowship exchange activities. Over the past ten years, there has been a high level of sensitivity for working collaboratively with European colleagues. This philosophy simply pervades each and every activity of the EU-US Task Force. Realistically, this means that there is a careful balance between the US and EU participation in all functions. The Brussels 'Celebration' workshop was no exception. The organizers anticipated funding more former U.S. trainees than actually attended the workshop and raised the necessary funds to accomplish this goal. However, the number of U.S. attendees needed to be tempered since the financial resources for our EU counterparts proved more difficult to obtain. In order to maintain the scholarly and political balance on the program of events, fewer U.S. attendees were invited. Details of the expenditure of DOE funds are provided in the Table 1. As indicated in the original proposal, funds were used to support both past trainees (Treves, Leigh, Buchan, Bender, Perez-Jimenez, Becker, and Methe) as well as Task Force members (Zylstra, Suflita, Wall). The general assessment by the trainees was that the past Task Force activities were crucial to their development as scientists. The prevailing feeling was that they wished that more individuals could profit from similar experiences. There was also a high degree of enthusiasm for the trainees to get involved in some way with the Task Force activities. That is, the celebration lead to the organization of our former trainees to serve in an advisory capacity for future endeavors. In addition, the gathering served as an opportunity to plan for another two week environmental biotechnology course at Rutgers

Biotechnology for the Environment

Joint US-European Community Pilot Program support for short-term exchanges of early career scientists

Microbial life in the deep terrestrial subsurface

The distribution and function of microorganisms is a vital issue in microbial ecology. The US Department of Energy`s Program, ``Microbiology of the Deep Subsurface,`` concentrates on establishing fundamental scientific information about organisms at depth, and the use of these organisms for remediation of contaminants in deep vadose zone and groundwater environments. This investigation effectively extends the Biosphere hundreds of meters into the Geosphere and has implications to a variety of subsurface activities

The Toxicity of Selected Gasoline Components to Glucose Methanogenesis by Aquifer Microorganisms

Six model hydrocarbons, representing various classes of chemicals found in gasoline, and methyl ethyl ketone, were assayed for their inhibitory effect on glucose methanogenesis in slurries prepared from aquifer sediments and ground water. Biogas (CH4 and CO2) production was monitored with an automated pressure transducer system. Benzene, 1-methyl naphthalene, and methyltert-butyl ether (MTBE) were found to have no inhibitory influence on biogas production rates at concentrations up to 71·7 mg/L. Similarly, octane, cyclohexane, indan, and methyl ethylketone (MEK) were found to have only marginal negative effects on the rate of biogas production in aquifer slurries, at concentrations ranging from 51·7 to 72·1 mg/L. Thus, gasoline components had low apparent toxicities to microorganisms responsible for glucose methanogenesis in aquifier slurries. As the concentrations of the assayed hydrocarbons are about 100 times those typically reported after an aquifer has been contaminated with gasoline, it is unlikely that individual hydrocarbons will substantially impact anaerobic metabolic processes

Reductive Dehalogenation of a Nitrogen Heterocyclic Herbicide in Anoxic Aquifer Slurries

We studied the metabolic fate of bromacil in anaerobic aquifer slurries held under denitrifying, sulfate-reducing, or methanogenic conditions. Liquid chromatograhy-mass spectrometry of the slurries confirmed that bromacil was debrominated under methanogenic conditions but was not degraded under the other incubation conditions. This finding extends the range of aryl reductive dehalogenation reactions to include nitrogen heterocyclic compounds

Anaerobic Biodegradation of Known and Potential Gasoline Oxygenates in the Terrestrial Subsurface

The octane enhancer tetraethyllead has been largely phased out of automobile fuels due to environmental and health concerns, and recent U.S. legislation mandates the reformulation of gasoline to increase its oxygen content. Oxygenates such as methyl tert-butyl ether (MTBE), ethanol, methanol, and tert-butyl alcohol provide both octane enhancement and oxygen content to gasolines. The use of such additives is believed to be environmentally acceptable since oxygenated fuels reduce the impact of hydrocarbon combustion on the atmosphere