The Desired Effect : Pontiac\u27s Rebellion and the Native American Struggle to Survive in Britain\u27s North American Conquest

Ravaged by war and in debt after its victory in the French and Indian War, Britain was not only recuperating, but rejoicing over the signing of the Treaty of Paris in 1763. This treaty officially ended the fighting and gave Britain all of the land east of the Mississippi River, formerly owned by the French. The ink on the treaty was barely dry when a new insurgence arose in British occupied North America. Native Americans, dissatisfied after the war with their position as conquered people and not as allies, rebelled collectively against British colonists and forts along the frontier. Before the war had started, the French had traded and lived among the Native Americans, but perhaps most importantly, they had given them presents to show respect and diplomacy. The Native Americans had grown accustomed to this act of friendliness and when Britain, in debt after the war, wanted to considerably reduce the number of gifts given, there were severe consequences. In 1763, the Native Americans led an insurgence, commonly called Pontiac’s Rebellion because of Pontiac, the Ottawa leader. This insurgence would culminate in the first extensive multi-tribal resistance to European colonization in America. In response to Britain’s new policies, the Native Americans took ten of their forts, which led not only to excess in conflict, but to the British exposing smallpox blankets onto the Native Americans

The expanding toolkit of translating ribosome affinity purification

Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a variety of behaviors in the brain, and to understand the molecular consequences on those cells due to experimental manipulations, ranging from drugs of abuse to disease-causing mutations. Since its inception, a variety of methodological advances are opening new avenues of investigation. These advances include a variety of new methods for targeting cells for translating ribosome affinity purification by features such as their projections or activity, additional tags and mouse reagents increasing the flexibility of the system, and new modifications of the method specifically focused on studying the regulation of translation. The latter includes methods to assess cell type-specific regulation of translation in specific subcellular compartments. Here, I provide a summary of these recent advances and resources, highlighting both new experimental opportunities and areas for future technical development.</jats:p

Control of Nucleotide Metabolism and Ribosomal Ribonucleic Acid Synthesis During Nitrogen Starvation of \u3cem\u3eEscherichia coli\u3c/em\u3e

Ribosomal ribonucleic acid (RNA) synthesis and ribonucleoside triphosphate metabolism were studied in cultures of Escherichia coli subjected to starvation for inorganic nitrogen. In a strain that was under stringent control, a 50-fold reduction in the formation of both 16S and 23S RNA was accompanied by a severe restriction on nucleotide biosynthesis. These inhibitions were relieved in part by incubating the starved cells with amino acids. This result suggests that regulation by the functional RNA control (RC) gene is involved in the effect. This suggestion was confirmed by showing that the effector of the stringent response, guanosine-5′-diphosphate-2′- or 3′-diphosphate (ppGpp), accumulated at the onset of starvation and disappeared immediately when the amino acids were added. Ribosomal RNA synthesis was severely restricted and the same nucleotide, ppGpp, accumulated at the onset of nitrogen starvation of a relaxed mutant too. These findings suggest that a control mechanism other than the one provided by the functional rel gene might operate to regulate RNA synthesis and that this mechanism is expressed through the synthesis of ppGpp