Evaluación de la germinación y vigor de alfalfa (Medicago sativa L.) Con rizobacterias y CuSO4·5H₂O

Los contaminantes metálicos en suelos y aguas representan un problema serio en crecimiento debido a las actividades antropogénicas. Algunas técnicas de fitorremediación con rizobacterias, pueden emplearse como una solución alternativa a la descontaminación de sitios afectados por metales. Este trabajo tuvo como objetivo evaluar la germinación de semilla de Alfalfa en soluciones con siete concentraciones de sulfato de cobre y, analizar el vigor y germinación de la Alfalfa inoculada con rizobacterias. Semillas de Alfalfa se germinaron en soluciones de sulfato de cobre, las semillas se inocularon con suspensiones bacterianas de dos cepas de Pseudomonas M40 y M67 y los porcentajes de germinación se determinaron y, se hicieron pruebas de vigor. El mayor porcentaje de germinación se logró a los siete días en las soluciones de sulfato de cobre 10-4 a 10-5 M, la inoculación de semillas de Alfalfa con la cepa M40 presentó 91.3% de germinación en dos días y la variedad Aragón inoculada con la cepa M40 presentó mayor crecimiento de tallo. Entre mayor fue la concentración de sulfato de cobre menor germinación se produce, sin embargo, si logra tolerar pequeñas concentraciones de sulfato de cobre. Medicago sativa es una alternativa viable para la descontaminación de sitios afectados por metales potencialmente tóxicos

EFFECT OF COPPER SULPHATE PENTAHYDRATE (CuSO4·5H₂O) AND RHIZOBACTERIA ON THE GERMINATION OF ROSELLE (Hibiscus sabdariffa L.)

Background: Heavy metals are perceived as the most persistent and dangerous. Cu is essential for plants, in excess it causes detrimental effects on plants and human health. Plant growth promoting bacteria have been mainly used in agriculture to promote seed germination, plant vigor and growth, and improve crop yields. Objective: To analyse the effect of germination in two roselle varieties that were inoculated with extracellular plant growth-promoting rhizobacteria of the genus Pseudomonas and in seven solutions of copper sulphate pentahydrate. Methodology: Roselle seeds were germinated in copper sulphate solutions, the seeds were inoculated with bacterial suspensions of two strains of Pseudomonas M40 and M67, the germination percentages were determined and vigour tests were also carried out. Results: The effect of copper sulphate on germination was significant in the interaction between main factors, but no significance was found in the effect of the strains and their interaction with the varieties. In the vigour tests, significance was detected between factors of stem dry weight, root and stem length, and strains. Implications: The tolerance of the seeds to copper sulphate, to know if it has an effect of promoting germination or, on the contrary, inhibits it. Conclusion: copper sulphate has the effect of promoting the germination of roselle seeds. Jamaica has a potential effect of phytoremediation of metals in combination with the inoculation of rhizobacteria that promote plant growth