T-cell immunology : the maths of memory

© Copyright Borghans and Ribeiro. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Mathematical modeling reveals that long-term immunological memory is maintained in a manner that is even more dynamic than previously thought.info:eu-repo/semantics/publishedVersio

The role of bedding in the formation of fault–fold structures,

Fold-fault structures within a major transpressional shear zone of the SW Iberian Massif were investigated by combining geological mapping, cross-section analysis and microtectonic studies. A significant example of contractional deformation is displayed in the Portalegre-Esperanc¸a Shear Zone (PESZ) where a heterogeneous Ordovician stratigraphic sequence, showing a strong competence contrast between quartzites, slates and quartzo-feldspathic rocks favoured strain localization and fault nucleation that controlled fold formation. The presence of pelitic layers within the thick-bedded quartzites had probably provided weakness zones that were more favourable for the strain localization than the previous foliation present in the quartzites. The quartzites and the quartzo-feldspathic rocks (granites and volcaniclastic rocks) accommodated heterogeneous high strain developing different degrees of mylonitization. The quartzites with protomylonitic textures are dominant and represent coarse-grained siliciclastic sediments that suffered metamorphism and partial dynamic recrystallization. Ultramylonites occur within discrete high-strain shear zones. It is probable that the strain localization in the PESZ involved both the effect of having layers of different competence and layers or stratigraphic contacts with rocks that experienced grain size reduction dominated by cataclasis and dislocation creep. Folds within quartzites with sub-horizontal to gently plunging hinges vary from closed to open in thick-bedded quartzites and from tight to closed in thin-bedded quartzites. Observed changes in structural style of deformed quartzites, slates and quartzo-feldspathic rocks are interpreted to result from the constraints imposed by the mechanical properties of the different lithologies. As the folds tightened during shortening, the alternating zones of contrasting competence favoured the disruption of the bedding. The deformation history in the PESZ was dominated by thrusting and strike-slip faulting along incompetent layers that locally developed staircase geometry and transected the lower Ordovician stratigraphic sequence. The kinematic model proposed for the PESZ is consistent with the models of transpressional shear zones stretched along strike with the development of coeval strike-slip and low angle oblique-slip faults associated to active buckle folds with axes parallel to the principal extension directio

A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes

Introduction: During evolution, blood-feeding arthropods developed a complex salivary mixture that can interfere with host haemostatic and immune response, favoring blood acquisition and pathogen transmission. Therefore, a survey of the salivary gland contents can lead to the identification of molecules with potent pharmacological activity in addition to increase our understanding of the molecular mechanisms underlying the hematophagic behaviour of arthropods. The southern house mosquito, Culex quinquefasciatus, is a vector of several pathogenic agents, including viruses and filarial parasites that can affect humans and wild animals. Results: Previously, a Sanger-based transcriptome of the salivary glands (sialome) of adult C. quinquefasciatus females was published based on the sequencing of 503 clones organized into 281 clusters. Here, we revisited the southern mosquito sialome using an Illumina-based RNA-sequencing approach of both male and female salivary glands. Our analysis resulted in the identification of 7,539 coding DNA sequences (CDS) that were functionally annotated into 25 classes, in addition to 159 long non-coding RNA (LncRNA). Additionally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and/or pathogen transmission. Conclusion: Together, these findings represent an extended reference for the identification and characterization of the proteins containing relevant pharmacological activity in the salivary glands of C. quinquefasciatus mosquitoes.This work was supported by the Intramural Research Program of the Division of Intramural Research (AI001246 and AI000810), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). This work utilized the computational resources of the NIH HPC Biowulf cluster (http://hpc.nih.gov). Open Access funding provided by the National Institutes of Health (NIH).S

A Remark on Lorentz Violation at Finite Temperature

We investigate the radiatively induced Chern-Simons-like term in four-dimensional field theory at finite temperature. The Chern-Simons-like term is temperature dependent and breaks the Lorentz and CPT symmetries. We find that this term remains undetermined although it can be found unambiguously in different regularization schemes at finite temperature.Comment: To appear in JHEP, 8 pages, 1 eps figure, minor changes and references adde

Interplay between the salience and the default mode network in a social-cognitive task toward a close other

Social cognition relies on two main subsystems to construct the understanding of others, which are sustained by different social brain networks. One of these social networks is the default mode network (DMN) associated with the socio-cognitive subsystem (i.e., mentalizing), and the other is the salience network (SN) associated with the socio-affective route (i.e., empathy). The DMN and the SN are well-known resting state networks that seem to constitute a baseline for the performance of social tasks. We aimed to investigate both networks' functional connectivity (FC) pattern in the transition from resting state to social task performance. A sample of 38 participants involved in a monogamous romantic relationship completed a questionnaire of dyadic empathy and underwent an fMRI protocol that included a resting state acquisition followed by a task in which subjects watched emotional videos of their romantic partner and elaborated on their partner's (Other condition) or on their own experience (Self condition). Independent component and ROI-to-ROI correlation analysis were used to assess alterations in task-independent (Rest condition) and task-dependent (Self and Other conditions) FC. We found that the spatial FC maps of the DMN and SN evidenced the traditional regions associated with these networks in the three conditions. Anterior and posterior DMN regions exhibited increased FC during the social task performance compared to resting state. The Other condition revealed a more limited SN's connectivity in comparison to the Self and Rest conditions. The results revealed an interplay between the main nodes of the DMN and the core regions of the SN, particularly evident in the Self and Other conditions.info:eu-repo/semantics/publishedVersio

Comparison of three assays for total and free PSA using hybritech and WHO calibrations

Publisher Copyright: © 2021 International Institute of Anticancer Research. All rights reserved.Background/Aim: Lack of interchangeability between prostate-specific antigen (PSA) assays could have a clinical impact. We compared PSA assays from different manufacturers and calibrations. Patients and Methods: A total of 233 men who underwent prostate biopsy (PSA: 2-10 ng/ml; Beckman Coulter Access® Hybritech® as reference) were enrolled. Total (tPSA) and free PSA (fPSA) were also measured using the Roche cobas® and the Abbott Architect® methods. Results: Roche tPSA values were ≈ 1% higher than Beckman, while Abbott values were ~5% lower. Roche had the highest diagnostic sensitivity (92%) compared to Beckman Coulter (87%) and Abbott (85%). Roche fPSA was ≈3% lower and Abbott ≈17% higher than that of Beckman. For the percentage of fPSA, Roche had the highest sensitivity (98%). Conclusion: Roche cobas® and Beckman Coulter Access® Hybritech® tPSA were almost interchangeable. While the agreement was acceptable for tPSA, this did not happen with fPSA and greater efforts for harmonization are required.publishersversionpublishe

The influence of structure and surface chemistry of carbon materials on the decomposition of hydrogen peroxide

Carbon materials with different structural and chemical properties, namely activated carbons, carbon xerogels, carbon nanotubes, graphene oxide, graphite and glycerol-based carbon materials, were tested under different operating conditions for their ability to catalyse hydrogen peroxide (H2O2) decomposition in aqueous solutions. Activated carbons treated with concentrated sulphuric acid (ACS) are the most active catalytic materials for H2O2 decomposition in most of the conditions studied, due to the presence of sulphur containing functional groups at their surface. In addition, ACS proved to be a stable catalyst in reutilization tests for H2O2 decomposition. Methanol was used as selective scavenger of hydroxyl radicals (HO center dot), to show that activated carbons with a markedly basic character lead to the highest yield of HO center dot formed during the H2O2 decomposition process (14%, after 150 min of reaction). Overall, from the mechanistic interpretation of H2O2 decomposition, it is concluded that the presence of sulphur containing functional groups at the surface of the activated carbons improves the removal of H2O2 in aqueous solutions, but, on the other hand, the selective decomposition of H2O2 via HO center dot formation is enhanced by the presence of basic active sites on the Carbon surface

Crustal growth and deformational processes in the northern

The aim of this article is to present a compilation of available information on the Évora Massif based on structural mapping, whole-rock geochemistry, recognition of metamorphic mineral assemblages, and geothermobarometry. In our view, transcurrent movements responsible for strong orogen-parallel stretching were dominant and had a major role in the geodynamic evolution of this part of Ossa-Morena zone (southwest Iberian Massif). Cadomian and Variscan orogenic events separated by a period of intense rifting were the cause for the composite distribution of zones with contrasting metamorphic paths, the structural complexity, the variety of lithological associations, and the sequence of deformation events and magmatism. The proposed geodynamic reconstruction for this segment of the northern Gondwana continental margin includes three main stages in chronological order: (1) Neoproterozoic accretion and continental magmatic arc developing, dismantling, and reworking, followed by late-“orogenic” magmatism; (2) Lower Paleozoic crustal thinning, block tilting, and mantle upwelling, induced by generalized rifting, leading to the formation of marine basins with carbonate platform sediments and thick accumulations of volcaniclastic and terrigenous sediments, contemporaneous with normal and enriched mid-oceanic ridge basalt–type magmatism; and (3) Upper Paleozoic transpressional orogenesis resulting from obliquity of convergence and the geometry of the involved blocks. The third stage includes the tectonic inversion of Lower Paleozoic basins, crustal thickening, the exhumation of high- to medium-pressure rocks and partial exhumation of high-grade metamorphic lithologies (controlled by local transtension and major detachments), the formation of synorogenic basins fi lled with volcanicsedimentary sequences, and fi nally, the emplacement of late Variscan granodiorites and granites

Genomic Insights Into the Ixodes Scapularis Tick Vector of Lyme Disease

Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ~57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host \u27questing\u27, prolonged feeding, cuticle synthesis, blood meal concentration, novel methods of haemoglobin digestion, haem detoxification, vitellogenesis and prolonged off-host survival. We identify proteins associated with the agent of human granulocytic anaplasmosis, an emerging disease, and the encephalitis-causing Langat virus, and a population structure correlated to life-history traits and transmission of the Lyme disease agent