Differences in larval morphology of allopatric isolated populations of the odontophrynus occidentalis group from western argentina

A morphological comparative study of two larval populations - one from Mendoza and the other an isolated one from Sierra Famatina (La Rioja) - of the Argentine toad, Odontophrynus occidentalis, is reported.Observações de campo e a analise cariologica de varias populates de O. occidentalis da região ocidental da Argentina, revelaram a existencia de diferenças bem marcadas numa população isolada da Serra de Famatina, em relação as demais populações conhecidas da especie.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Differences in larval morphology of allopatric isolated populations of the odontophrynus occidentalis group from western argentina

A morphological comparative study of two larval populations - one from Mendoza and the other an isolated one from Sierra Famatina (La Rioja) - of the Argentine toad, Odontophrynus occidentalis, is reported.Observações de campo e a analise cariologica de varias populates de O. occidentalis da região ocidental da Argentina, revelaram a existencia de diferenças bem marcadas numa população isolada da Serra de Famatina, em relação as demais populações conhecidas da especie.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Remarks on some adaptive ecological trends of Pelobates cultripes from Portugal: thermal requirement, rate of development and water regulation

Spawning, tadpole biology, rate of development, tolerance to water loss and water absorption, of Pelobates cultripes from Portugal, were studied, having in mind similar researchs carried out on western American spadefoots.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Differences in larval morphology of allopatric isolated populations of the odontophrynus occidentalis group from western argentina

A morphological comparative study of two larval populations - one from Mendoza and the other an isolated one from Sierra Famatina (La Rioja) - of the Argentine toad, Odontophrynus occidentalis, is reported.Observações de campo e a analise cariologica de varias populates de O. occidentalis da região ocidental da Argentina, revelaram a existencia de diferenças bem marcadas numa população isolada da Serra de Famatina, em relação as demais populações conhecidas da especie.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Osteological differences in allopatric populations of the Odontophrynus occidentalis group from Western Argentina

An osteological comparison of new-metamorphosed specimens from three populations of the O. occidentalis group was carried out. The results confirm the accentuated differentiation of the Sierra Famatina population.Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Investigating the effect of target of rapamycin kinase inhibition on the Chlamydomonas reinhardtii phosphoproteome: from known homologs to new targets

Recuperado de: https://www.biorxiv.org/content/10.1101/310102v1Target of rapamycin (TOR) kinase is a conserved regulator of cell growth whose activity is modulated in response to nutrients, energy and stress. Key proteins involved in the pathway are conserved in the model photosynthetic microalga Chlamydomonas reinhardtii, but the substrates of TOR kinase and downstream signaling network have not been elucidated. Our study provides a new resource for investigating the phosphorylation networks governed by the TOR kinase pathway in Chlamydomonas. We used quantitative phosphoproteomics to investigate the effects of inhibiting Chlamydomonas TOR kinase on dynamic protein phosphorylation. Wild-type and AZD-insensitive Chlamydomonas strains were treated with TOR-specific chemical inhibitors (rapamycin, AZD8055 and Torin1), after which differentially affected phosphosites were identified. Our quantitative phosphoproteomic dataset comprised 2547 unique phosphosites from 1432 different proteins. Inhibition of TOR kinase caused significant quantitative changes in phosphorylation at 258 phosphosites, from 219 unique phosphopeptides. Our results include Chlamydomonas homologs of TOR signaling-related proteins, including a site on RPS6 with a decrease in phosphorylation. Additionally, phosphosites on proteins involved in translation and carotenoid biosynthesis were identified. Follow-up experiments guided by these phosphoproteomic findings in lycopene beta/epsilon cyclase showed that carotenoid levels are affected by TORC1 inhibition and carotenoid production is under TOR control in algae.National Science Foundation CAREER MCB-155252

Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in chlamydomonas

Fatty acids are synthesized in the stroma of plant and algal chloroplasts by the fatty acid synthase complex. Newly synthesized fatty acids are then used to generate plastidial lipids that are essential for chloroplast structure and function. Here, we show that inhibition of fatty acid synthesis in the model alga Chlamydomonas reinhardtii activates autophagy, a highly conserved catabolic process by which cells degrade intracellular material under adverse conditions to maintain cell homeostasis. Treatment of Chlamydomonas cells with cerulenin, a specific fatty acid synthase inhibitor, stimulated lipidation of the autophagosome protein ATG8 and enhanced autophagic flux. We found that inhibition of fatty acid synthesis decreased monogalactosyldiacylglycerol abundance, increased lutein content, down-regulated photosynthesis, and increased the production of reactive oxygen species. Electron microscopy revealed a high degree of thylakoid membrane stacking in cerulenin-treated cells. Moreover, global transcriptomic analysis of these cells showed an up-regulation of genes encoding chloroplast proteins involved in protein folding and oxidative stress and the induction of major catabolic processes, including autophagy and proteasome pathways. Thus, our results uncovered a link between lipid metabolism, chloroplast integrity, and autophagy through a mechanism that involves the activation of a chloroplast quality control system.Ministerio de Economía y Competitividad BFU2015-68216-PJunta de Andalucía CVI-7336, BIO2015-74432-JI

Kinetics and crystallization path of a Fe-based metallic glass alloy

The thermal stability and the quantification of the different transformation processes involved in the overall crystallization of the Fe50Cr15Mo14C15B6 amorphous alloy were investigated by several characterization techniques. Formation of various metastable and stable phases during the devitrification process in the sequence a-Fe, ¿-Cr6Fe18Mo5, M23(C,B)6, M7C3, ¿-Fe3Mo3C and FeMo2B2 (with M = Fe, Cr, Mo), was observed by in-situ synchrotron high energy X-ray diffraction and in-situ transmission electron microscopy. By combining these techniques with differential scanning calorimetry data, the crystallization states and their temperature range of stability under continuous heating were related with the evolution of the crystallized fraction and the phase sequence as a function of temperature, revealing structural and chemical details of the different transformation mechanisms.Postprint (published version

Further immunological evidence for the validity of the family Bombinidae (Amphibia Salientia)

New immunological data obtained using Libby’s photron-reflectro- metric technique confirms that Discoglossus belongs to a difíerent family (Discoglossidae Giinther, 1858) from that of Alytes and Bombina (Bombi- nidae Fitzinger, 1826).Nuovi dati immunologici ottenuti con la técnica fotronriflettometrica di Libby confermano che Discoglossus appartiene a una famiglia (Discoglossidae Giinther, 1858) diversa da quella di Alytes e di Bombina (Bombi- nidae Fitzinger, 1826).Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse

Further immunological evidence for the validity of the family Bombinidae (Amphibia Salientia)

New immunological data obtained using Libby’s photron-reflectro- metric technique confirms that Discoglossus belongs to a difíerent family (Discoglossidae Giinther, 1858) from that of Alytes and Bombina (Bombi- nidae Fitzinger, 1826).Nuovi dati immunologici ottenuti con la técnica fotronriflettometrica di Libby confermano che Discoglossus appartiene a una famiglia (Discoglossidae Giinther, 1858) diversa da quella di Alytes e di Bombina (Bombi- nidae Fitzinger, 1826).Material digitalizado en SEDICI gracias a la colaboración del Dr. Jorge Williams (FCNM-UNLP).Facultad de Ciencias Naturales y Muse