Potential Role of Sequential Solid-State and Submerged-Liquid Fermentations in a Circular Bioeconomy

An efficient processing of organic solid residues will be pivotal in the development of the circular bioeconomy. Due to their composition, such residues comprise a great biochemical conversion potential through fermentations. Generally, the carbohydrates and proteins present in the organic wastes cannot be directly metabolized by microorganisms. Thus, before fermentation, enzymes are used in a hydrolysis step to release digestible sugars and nitrogen. Although enzymes can be efficiently produced from organic solid residues in solid-state fermentations (SsF), challenges in the development and scale-up of SsF technologies, especially bioreactors, have hindered a wider application of such systems. Therefore, most of the commercial enzymes are produced in submerged-liquid fermentations (SmF) from expensive simple sugars. Instead of independently evaluating SsF and SmF, the review covers the option of combining them in a sequential process in which, enzymes are firstly produced in SsF and then used for hydrolysis, yielding a suitable medium for SmF. The article reviews experimental work that has demonstrated the feasibility of the process and underlines the benefits that such combination has. Finally, a discussion is included which highlights that, unlike typically perceived, SsF should not be considered a counterpart of SmF but, in contrast, the main advantages of each type of fermentation are accentuated in a synergistic sequential SsF-SmF

First record of Clavularia carpediem Weinberg, 1986 (Anthozoa, Stolonifera) since its original description: taxonomical, zoogeographical and bathymetrical data

First record of Clavularia carpediem Weinberg, 1986 (Anthozoa, Stolonifera) since its original description: taxonomical, zoogeographical and bathymetrical data.- The stoloniferous octocoral Clavularia carpediem Weinberg, 1986 has been found in the european side of the Strait of Gibraltar. This especies is only known from the Alboran Sea. The distinguishing features of this species are the presence of sclerites in the tentacles, a very ornamented crown and the size range of the two principal groups of sclentes: thick spindles with complex tubercles and more slender ones, with simple tubercles, that occurs in the anthosteles and stolons. The extemal morphology of Clavularia carpediem Weinberg, 1986 from the Strait of Gibraltar and the variability of the sclentes are described. Some aspects related to its habitat, biology and zoogeography are discussed

New records of copepods associated with marine invertebrates from the Strait of Gibraltar and nearby areas

In this work, 16 species of commensal or parasitic copepods associated with marine invertebrates from the Strait of Gibraltar are recorded. Eleven species are included into the order Cyclopoida, three into Poecilostomatoida, one into Siphonostomatoida, and another into a family incertae sedis. They have been found mainly associated with Ascidians, but also in Pelecypods, Polychaetes, Opisthobranch molluscs, Ophiuroids and Anthozoans. Among these, 13 are new records for the Iberian peninsula fauna, and three are also new for the African coasts. Moreover five species, Haplostomella malacocera Chatton & Harant, 1924; Haplostomella tuberculata Chatton & Harant, 1924; Periproctia falsiarcuata Stock, 1967; Anthessius arcuatus López-González, Conradi, Naranjo & García-Gómez, 1992 and Botryllophilus conicus Conradi, López-González & García-Gómez, 1994, are recorded for the first time since their original descriptions. Furthermore, new hosts and bathymetrical data of most of the species are provided

Complicaciones postanestesicas en pacientes sometidos a cirugías de emergencia en las salas de recuperación del hospital Antonio Lenin Fonseca en el segundo semestre del año 2014

La labor anestésica no solo termina con la finalización de procedimiento quirúrgico. Sino que también aborda el tiempo evolutivo del paciente en la Unidad De Cuidados Postanestesicos. Ya que un buen manejo anestésico puede ser opacado por una mala recuperació

Production of lactic acid from pasta wastes using a biorefinery approach

A total of 398 kt of pasta waste (PW), generated during the production process of pasta, were produced in 2021. Due to its chemical composition and practically zero cost, PW has already been studied as a raw material for the production of lactic acid (LA) through fermentations. The main objective of this article was to improve the economic viability of the process by replacing commercial enzymes, necessary for starch hydrolysis in PW, with raw enzymes also produced from wastes. Enzyme synthesis was achieved through solid-state fermentation (SsF) of wheat bran by Aspergillus awamori or Aspergillus oryzae at various moisture contents. The maximum amylase activity (52 U/g dry solid) was achieved after 2 days of fermentation with A. awamori at 60% of moisture content. After that, the enzymes were used to hydrolyse PW, reaching 76 g/L of total sugars, 65 g/L of glucose and a yield of 0.72 gglu/gds with the enzymes produced by A. awamori. Subsequently, the hydrolysate was fermented into LA using Bacillus coagulans A559, yielding 52 g/L and 49 g/L with and without yeast extract, respectively. Remarkably, compared to the process with commercial enzymes, a higher LA yield was reached when enzymes produced by SsF were added (0.80 gLA/gglu). Furthermore, the productivities between the two processes were similar (around 3.9 g/L/h) which highlights that yeast extract is not necessary when using enzymes produced by SsF

A survey of the genera Sarcodictyon Forbes, 1847, and rolandia Lacaze-Duthiers, 1900, (anthozoa: octocorallia) in the North-eastern Atlantic and the Mediterranean

Sarcodictyon catenatum Forbes, 1847, is a common species in the Atlantic and the Mediterranean. However, often it has been mistaken for Rolandia coralloides Lacaze-Duthiers, 1900. On the basis of British and Mediterranean material we studied the main characters for distinguishing S. catenatum. We consider Rolandia coralloides Lacaze-Duthiers, 1900, to be a valid species and we assign to it all the material named Rolandia rosea by S. Weinberg (1978). Furthermore, a histological study confirmed that R. coralloides typically forms groups of polyps embedded in a common coenenchyme. Therefore, the possibility of transferring R. coralloides from Stolonifera to Alcyonacea is discussed. Evagorgia rosea Philippi, 1842, is here considered to be a nomen dubium

From Upstream to Purification : Production of Lactic Acid from the Organic Fraction of Municipal Solid Waste

The implementation of an efficient and sustainable management of the organic fraction of municipal solid wastes (OFMSW) is a topic of intensive discussion in EU countries. Recently, the OFMSW has been investigated as a potential substrate for the production of lactic acid (LA) through fermentation. Nevertheless, none of the reports available in the literature covers all the stages of the conversion process. The present research article is a comprehensive study which includes the upstream, fermentation and downstream for the conversion of OFMSW into LA. Several batches of OFMSW were analysed for the evaluation of sugars released and LA content before the fermentation. Fermentations were performed to study the effect of hydrolysate quality on the LA production using Bacillus coagulans A166. Purification of LA, based on electrodialysis, was carried out after pilot scale fermentation of OFMSW hydrolysates. Results showed that variations in the concentrations of sugars and LA are observed from batch to batch of OFMSW. More specifically, LA can reach high concentrations even before the substrates are hydrolysed, limiting the potential applications of the final product due to low enantiomeric purities. In general, fermentations of the hydrolysate were efficient, with conversion yields of 0.65 g g−1 without the addition of extra nutrients. Downstream is still a challenging stage of the process. A LA recovery of 55% was obtained, with the most significant losses observed during the micro- and nanofiltrations. Overall, a conversion of 10% from OFMSW substrate (dry basis) to LA was achieved