Social Diversification of Overweight and Obesity in Adults in Poland

Overweight and obesity are connected with environmental factors to a greater degree than with genetic and psychological factors, because in their aetiology the focus is mostly on two factors, i.e. inappropriate diet and a sedentary lifestyle. This is an increasingly widespread phenomenon that harms the health and quality of life in adults, and also contributes to a decrease in their efficiency at work. The study aimed to evaluate the social diversification of overweight and obesity in adults living in different social environments in Swietokrzyskie region of Poland. The study was based on a questionnaire and observations, and encompassed 517 women and 515 men aged between 20 and 59 years. The questionnaire included questions about the characteristics of the social and family environment; while the observation involved anthropometric measurements of the basic somatic traits that were used to calculate the Body Mass Index (BMI), Waist-to-Hip Ratio (WHR) and Waist-to-Height Ratio (WHtR), which in turn were used to evaluate the occurrence of overweight and obesity. The incidence of overweight and obese adults showed a clear increase with age and displayed considerable social diversification, especially in the case of the women, among whom a better financial situation and a higher level of education correlated with a more slender body shape and a smaller increase in the incidence of overweight and obesity. The increase in the number of overweight and obese adult persons who lived in the different social environments in Poland was only slightly smaller than the increase in other European countries. Overall, the social diversification of overweight and obesity in adult persons indicates that Polish women are more health-aware than men

Development and application of CRISPR-based genetic tools in Bacillus species and Bacillus phages

Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool. Since its discovery as an adaptive immune system in prokaryotes, it has been applied in many different research fields including biotechnology and medical sciences. The high demand for rapid, highly efficient and versatile genetic tools to thrive in bacteria-based cell factories accelerates this process. This review mainly focuses on significant advancements of the CRISPR system in Bacillus subtilis, including the achievements in gene editing, and on problems still remaining. Next, we comprehensively summarize this genetic tool's up-to-date development and utilization in other Bacillus species, including B. licheniformis, B. methanolicus, B. anthracis, B. cereus, B. smithii and B. thuringiensis. Furthermore, we describe the current application of CRISPR tools in phages to increase Bacillus hosts' resistance to virulent phages and phage genetic modification. Finally, we suggest potential strategies to further improve this advanced technique and provide insights into future directions of CRISPR technologies for rendering Bacillus species cell factories more effective and more powerful

Engineering of Multiple Modules to Improve Amorphadiene Production in Bacillus subtilis Using CRISPR-Cas9

Engineering strategies to improve terpenoids production in Bacillus subtilis mainly focus on 2C-methyl-D-erythritol-4-phosphate (MEP) pathway overexpression. To systematically engineer the chassis strain for higher amorphadiene (precursor of artemisinin) production, a clustered regularly interspaced short palindromic repeat-Cas9 (CRISPR-Cas9) system was established in B. subtilis to facilitate precise and efficient genome editing. Then, this system was employed to engineer three more modules to improve amorphadiene production, including the terpene synthase module, the branch pathway module, and the central metabolic pathway module. Finally, our combination of all of the useful strategies within one strain significantly increased extracellular amorphadiene production from 81 to 116 mg/L after 48 h flask fermentation without medium optimization. For the first time, we attenuated the FPP-derived competing pathway to improve amorphadiene biosynthesis and investigated how the TCA cycle affects amorphadiene production in B. subtilis. Overall, this study provides a universal strategy for further increasing terpenoids' production in B. subtilis by comprehensive and systematic metabolic engineering