Identification of the optimal hiit protocol for fatigue resistance in adolescent athletes: a randomized controlled trial

The combat sports athletes developed great gains in both muscular function and fatigue resistance by utilizing high-intensity interval training (HIIT). However, it has not been investigated fully whether different work-to-rest ratios of HIIT show the effectiveness on muscle function in adolescent athletes. The purpose of the study was to compare different work-to-rest ratios by applying different rest times in response to the identical work time during HIIT on muscle function in adolescent taekwondo athletes. Forty-seven adolescent male taekwondo athletes (mean age: 16.7±0.8 years) were randomly assigned to the control group (n=11) vs. three HIIT groups by work-to-rest ratios; (1) 1:2 [30s:60s] (n=12), (2) 1:4 [30s:120s] (n=12), and (3) 1:8 [30s:240s] (n=12). All groups completed 10 experimental sessions over four weeks, while the control group maintained their regular taekwondo training. Muscular functions were measured by assessing isokinetic muscle strength and endurance of the knee extensor and flexor. The participants performed three sets of twenty maximal extension and flexion contractions at 120°s-1 with a 1-min interval between the sets for fatigue resistance. Blood samples were collected to measure free-testosterone, cortisol, creatine kinase, and urea as stress-to-recovery indicators. A positive effect on improving muscle fatigue resistance was observed at the first set of assessments in the HIIT with 1:4 (Δ10.2%, p<.05) and 1:8 groups (Δ8.6%, p<.05). Additionally, the 1:4 group exhibited fatigue resistance improvement in the second set (Δ7.7%, p<.01) without any changes of stress-to-recovery indicators, while the other groups did not show any improvement. The 30s all-out work with 120s rest time, lasting over a brief 4-week period, improved participants’ fatigue resistance. A certain amount of rest time between high-intense movements is required to optimize muscle development in adolescent athletes compared with insufficient rest time

High frequencies of Y-chromosome haplogroup O2b-SRY465 lineages in Korea: a genetic perspective on the peopling of Korea

<p>Abstract</p> <p>Background</p> <p>Koreans are generally considered a Northeast Asian group, thought to be related to Altaic-language-speaking populations. However, recent findings have indicated that the peopling of Korea might have been more complex, involving dual origins from both southern and northern parts of East Asia. To understand the male lineage history of Korea, more data from informative genetic markers from Korea and its surrounding regions are necessary. In this study, 25 Y-chromosome single nucleotide polymorphism markers and 17 Y-chromosome short tandem repeat (Y-STR) loci were genotyped in 1,108 males from several populations in East Asia.</p> <p>Results</p> <p>In general, we found East Asian populations to be characterized by male haplogroup homogeneity, showing major Y-chromosomal expansions of haplogroup O-M175 lineages. Interestingly, a high frequency (31.4%) of haplogroup O2b-SRY465 (and its sublineage) is characteristic of male Koreans, whereas the haplogroup distribution elsewhere in East Asian populations is patchy. The ages of the haplogroup O2b-SRY465 lineages (~9,900 years) and the pattern of variation within the lineages suggested an ancient origin in a nearby part of northeastern Asia, followed by an expansion in the vicinity of the Korean Peninsula. In addition, the coalescence time (~4,400 years) for the age of haplogroup O2b1-47z, and its Y-STR diversity, suggest that this lineage probably originated in Korea. Further studies with sufficiently large sample sizes to cover the vast East Asian region and using genomewide genotyping should provide further insights.</p> <p>Conclusions</p> <p>These findings are consistent with linguistic, archaeological and historical evidence, which suggest that the direct ancestors of Koreans were proto-Koreans who inhabited the northeastern region of China and the Korean Peninsula during the Neolithic (8,000-1,000 BC) and Bronze (1,500-400 BC) Ages.</p

Safety and efficacy study of laparoscopic or robotic radical surgery using an endoscopic stapler for inhibiting tumour spillage of cervical malignant neoplasms evaluating survival (SOLUTION): a multi-centre, open-label, single-arm, phase II trial protocol

The Laparoscopic Approach to Cervical Cancer trial and Surveillance, Epidemiology, and End Results program database study demonstrated that minimally invasive radical hysterectomy was inferior to abdominal radical hysterectomy in terms of disease recurrence and survival. Among risk factors related to poor prognosis after minimally invasive surgery (MIS), tumour spillage during intracorporeal colpotomy became a significant issue. Thus, we designed this trial to evaluate the efficacy and safety of minimally invasive radical hysterectomy using an endoscopic stapler for early-stage cervical cancer. This trial is a prospective, multi-centre, open-label, single-arm, non-inferiority phase II study. The nine organisations will participate in this trial after the approval of the institutional review board. Major eligibility criteria include women aged 20 years or older with cervical cancer stage IB1 squamous cell carcinoma, adenocarcinoma, or adenosquamous carcinoma according to the revised 2009 FIGO staging system who will undergo type B2 or C hysterectomy by MIS. The primary endpoint is the 4.5-year disease-free survival (DFS) rate between abdominal radical hysterectomy and MIS using an endoscopic stapler. For calculating the sample size, we hypothesised that the 4.5-year DFS rate after MIS using an endoscopic stapler is assumed to be the same after abdominal radical hysterectomy at 90.9%, and the non-inferiority margin was 7.2%. When we consider a three-year accrual and 4.5-year follow-up, at least 13 events must happen, requiring a total of 111 patients assuming a statistical power of 80% and the one-tailed test of 5% significance. A total of 124 patients is needed, considering a drop-out rate of 10%. We expect intracorporeal colpotomy using an endoscopic stapler may prevent tumour spillage during MIS for stage IB1 cervical cancer, showing a comparable prognosis with abdominal radical surgery.This study was supported by Johnson & Johnson. The funder has no role in study design, writing of the manuscript and the decision to submit the report for publication

Printing-Based Assay and Therapy of Antioxidants

Antioxidants are essential in regulating various physiological functions and oxidative deterioration. Over the past decades, many researchers have paid attention to antioxidants and studied the screening of antioxidants from natural products and their utilization for treatments in diverse pathological conditions. Nowadays, as printing technology progresses, its influence in the field of biomedicine is growing significantly. The printing technology has many advantages. Especially, the capability of designing sophisticated platforms is useful to detect antioxidants in various samples. The high flexibility of 3D printing technology is advantageous to create geometries for customized patient treatment. Recently, there has been increasing use of antioxidant materials for this purpose. This review provides a comprehensive overview of recent advances in printing technology-based assays to detect antioxidants and 3D printing-based antioxidant therapy in the field of tissue engineering. This review is divided into two sections. The first section highlights colorimetric assays using the inkjet-printing methods and electrochemical assays using screen-printing techniques for the determination of antioxidants. Alternative screen-printing techniques, such as xurography, roller-pen writing, stamp contact printing, and laser-scribing, are described. The second section summarizes the recent literature that reports antioxidant-based therapy using 3D printing in skin therapeutics, tissue mimetic 3D cultures, and bone tissue engineering

Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries

In this study, an inkjet bioprinting-based high-throughput screening (HTS) system was designed and applied for the first time to a catecholpyrimidine-based small molecule library to find hit compounds that inhibit c-Jun NH2-terminal kinase1 (JNK1). JNK1 kinase, inactivated MAPKAPK2, and specific fluorescent peptides along with bioink were printed on parchment paper under optimized printing conditions that did not allow rapid evaporation of printed media based on Triton-X and glycerol. Subsequently, different small compounds were printed and tested against JNK1 kinase to evaluate their degree of phosphorylation inhibition. After printing and incubation, fluorescence intensities from the phosphorylated/nonphosphorylated peptide were acquired for the % phosphorylation analysis. The IM50 (inhibitory mole 50) value was determined as 1.55 x 10(-15) mol for the hit compound, 22. Thus, this work demonstrated that inkjet bioprinting-based HTS can potentially be adopted for the drag discovery process using small molecule libraries, and cost-effective HTS can be expected to be established based on its low nano- to picoliter printing volume.Y

Legislative Documents

Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli

In this work we investigated the antibacterial properties of differently shaped silver nanoparticles against the gram-negative bacterium Escherichia coli, both in liquid systems and on agar plates. Energy-filtering transmission electron microscopy images revealed considerable changes in the cell membranes upon treatment, resulting in cell death. Truncated triangular silver nanoplates with a {111} lattice plane as the basal plane displayed the strongest biocidal action, compared with spherical and rod-shaped nanoparticles and with Ag(+) (in the form of AgNO(3)). It is proposed that nanoscale size and the presence of a {111} plane combine to promote this biocidal property. To our knowledge, this is the first comparative study on the bactericidal properties of silver nanoparticles of different shapes, and our results demonstrate that silver nanoparticles undergo a shape-dependent interaction with the gram-negative organism E. coli