Favored serum albumin level and ICF volume after use of 1.1% aminoacid based peritoneal dialysis(PD) solution

Aminoacid based PD solution (AAD) has been shown to induce positive nitrogen balance and improve nutritional markers of malnourished patients. But its effcets on body fluid composition and various nutritional markers are contradictory. Nutritional markers may influenced by patient's ECF volume status. So we evaluate effects of AAD on nutritional markers and body composition by analysis using multi-frequency bioimpedance analyzer. 35 PD patients(>6months duration of CAPD) were prospectively randomized to 17 AAD(Nutrineal, one time use/day) and 18 GD group(keep their glucose based PD solution). After 3 months follow up, AAD group showed marginally increased body weight and fat mass, decreased ECF volume(12.45±0.54Lvs 12.10±0.57L, p=0.06), no changed ICF volume(22.2±0.9Lvs 22.3±0.9L, p>0.05) and marginally increased drainage volume(8.77±0.76Lvs 9.12±0.83L, p=0.09). AAD group also showed favored several markers include nPCR(1.59±0.07vs 1.98±0.08, p=0.00), BUN and albumin level (3.54±0.11 vs 3.74±0.11, p=0.02). Although serum albumin level was increased, correction with ECF volume(albumin level X ECF volume) makes it no difference (43.45±2.13vs 44.80±2.28, p=0.14). Furthermore △albumin vs △ECF showed negative correlation pattern(r=-0.46, p=0.07) that means serum albumin change was influenced by ECF volume change. In conclusion, AAD treatment improved markers of better nutritional status. However the change in serum albumin level was influenced by patient's ECF volume status, which can partially explain contradictory effect of aminoacid based PD solution on serum albumin level

Dedicated preparation for in situ transmission electron microscope tensile testing of exfoliated graphene

Graphene, which is one of the most promising materials for its state-of-the-art applications, has received extensive attention because of its superior mechanical properties. However, there is little experimental evidence related to the mechanical properties of graphene at the atomic level because of the challenges associated with transferring atomically-thin two-dimensional (2D) materials onto microelectromechanical systems (MEMS) devices. In this study, we show successful dry transfer with a gel material of a stable, clean, and free-standing exfoliated graphene film onto a push-to-pull (PTP) device, which is a MEMS device used for uniaxial tensile testing in in situ transmission electron microscopy (TEM). Through the results of optical microscopy, Raman spectroscopy, and TEM, we demonstrate high quality exfoliated graphene on the PTP device. Finally, the stress???strain results corresponding to propagating cracks in folded graphene were simultaneously obtained during the tensile tests in TEM. The zigzag and armchair edges of graphene confirmed that the fracture occurred in association with the hexagonal lattice structure of graphene while the tensile testing. In the wake of the results, we envision the dedicated preparation and in situ TEM tensile experiments advance the understanding of the relationship between the mechanical properties and structural characteristics of 2D materials

A Keck/LRIS Spatially-Resolved Spectroscopic Study of a LINER Galaxy SDSS J091628.05+420818.7

Using spatially-resolved spectra obtained with the Low Resolution Imaging Spectrometer at the Keck I telescope, we investigate the nature of ionizing sources and kinematic properties of emission-line gas in a LINER galaxy SDSS J091628.05+420818.7, which is a nearby (z = 0.0241) and bright (M_r = -20.2) early-type galaxy. After subtracting stellar absorption features using a combination of simple stellar population models, we measure the flux, line-of-sight velocity, and velocity dispersion of four emission lines, i.e., H{\alpha}, H{\beta}, [O III] {\lambda}5007, and [N II] {\lambda}6584, to study radial change of emission-line fluxes and velocities. Compared to the point-spread-function of the observation, the emission-line region is slightly extended but comparable to the seeing size. The central concentration of emission-line gas suggests that ionization is triggered by a nuclear source, excluding old stellar population as ionizing sources. We find that emission-line gas is counter-rotating with respect to stellar component and that the [O III] {\lambda}5007 line is blueshifted compared to other emission lines, possibly due to an outflow.Comment: 10 pages, 8 figures; Accepted for publication in Ap

The C-terminal region of Bfl-1 sensitizes non-small cell lung cancer to gemcitabine-induced apoptosis by suppressing NF-κB activity and down-regulating Bfl-1

Gemcitabine is used to treat several cancers including lung cancer. However, tumor cells often escape gemcitabine-induced cell death via various mechanisms, which include modulating bcl-2 family members and NF-κB activation. We previously reported that the C-terminal region of Bfl-1 fused with GFP (BC) is sufficient to induce apoptosis in 293T cells. In the present study, we investigated the anti-tumor effect of combined BC gene therapy and gemcitabine chemotherapy in vitro and in vivo using non-small cell lung cancer cell lines and a xenograft model. Cell lines were resistant to low dose gemcitabine (4-40 ng/ml), which induced NF-κB activation and concomitant up-regulation of Bfl-1 (an NF-κB-regulated anti-apoptotic protein). BC induced the apoptosis of A549 and H157 cells with caspase-3 activation. Furthermore, co-treatment with BC and low dose gemcitabine synergistically and efficiently induced mitochondria-mediated apoptosis in these cells. When administered alone or with low dose gemcitabine, BC suppressed NF-κB activity, inhibited the nuclear translocation of p65/relA, and down-regulated Bfl-1 expression. Furthermore, direct suppression of Bfl-1 by RNA interference sensitized cells to gemcitabine-induced cell death, suggesting that Bfl-1 importantly regulates lung cancer cell sensitivity to gemcitabine. BC and gemcitabine co-treatment also showed a strong anti-tumor effect in a nude mouse/A549 xenograft model. These results suggest that lung cancer cells become resistant to gemcitabine via NF-κB activation and the subsequent overexpression of Bfl-1, and that BC, which has both pro-apoptotic and NF-κB inhibitory effects, could be harnessed as a gene therapy to complement gemcitabine chemotherapy in non-small cell lung cancer

Clinical Comparison of the Auditory Steady-State Response with the Click Auditory Brainstem Response in Infants

ObjectivesOur goal was to determine the effectiveness of using the auditory steady state response (ASSR) as a measure of hearing thresholds in infants who are suspected of having significant hearing loss, as compared with using the click-auditory brainstem response (C-ABR).MethodsWe retrospectively analyzed the audiologic profiles of 76 infants (46 boys and 30 girls, a total of 151 ears) who ranged in age from 1 to 12 months (average age: 5.7 months). The auditory evaluations in 76 infants who were suspected of having hearing loss were done via the C-ABR and ASSR. In addition, for reference, the mean ASSR thresholds were compared to those of 39 ears of infants and 39 ears of adults with normal hearing at 0.5, 1, 2, and 4 kHz.ResultsThe highest correlation between the C-ABR and ASSR thresholds was observed at an average of 2-4 kHz (r=0.94). On comparison between the hearing of infants and adults at 0.5, 1, 2, and 4 kHz, the mean ASSR threshold in infants was 12, 7, 8, and 7 dB higher, respectively, than that in adults.ConclusionASSR testing may provide additional audiometric information for accurately predicting the hearing sensitivity, and this is essential for the management of infants with severe to profound hearing loss