Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains

<p>Abstract</p> <p>Background</p> <p>The Ada-dependent adaptive response system in <it>Escherichia coli </it>is important for increasing resistance to alkylation damage. However, the global transcriptional and translational changes during this response have not been reported. Here we present time-dependent global gene and protein expression profiles following treatment with methyl methanesulfonate (MMS) in <it>E. coli </it>W3110 and its <it>ada </it>mutant strains.</p> <p>Results</p> <p>Transcriptome profiling showed that 1138 and 2177 genes were differentially expressed in response to MMS treatment in the wild-type and mutant strains, respectively. A total of 81 protein spots representing 76 nonredundant proteins differentially expressed were identified using 2-DE and LC-MS/MS. In the wild-type strain, many genes were differentially expressed upon long-exposure to MMS, due to both adaptive responses and stationary phase responses. In the <it>ada </it>mutant strain, the genes involved in DNA replication, recombination, modification and repair were up-regulated 0.5 h after MMS treatment, indicating its connection to the SOS and other DNA repair systems. Interestingly, expression of the genes involved in flagellar biosynthesis, chemotaxis, and two-component regulatory systems related to drug or antibiotic resistance, was found to be controlled by Ada.</p> <p>Conclusion</p> <p>These results show in detail the regulatory components and pathways controlling adaptive response and how the related genes including the Ada regulon are expressed with this response.</p

Ethical Attitudes toward Buying and Selling Counterfeits: Beneficial Lies, Belief in Fairness, and Non-monetary Values

The purpose of the study is to examine how values, explicitly (1) tolerance of beneficial lies, (2) belief in fairness, and (3) belief in non-monetary values, are related to attitudes toward buying and selling counterfeits

4.EAFES International Congress in Mokpo, Korea

Korea Forest Research InstituteProject Number 14404021, Peport of Research Project ; Grant-in-Aid for Scientific Research(B)(2), from April 2002 to March 2006, Edited by Muramoto,Ken-ichiroKamata, NaotoKawanishi, TakuyaKubo, MamoruLiu, JiyuanLee, Kyu-Sung , 人工衛星データ活用のための東アジアの植生調査、課題番号14404021, 平成14年度～平成17年度科学研究費補助金, 基盤研究（B)(2)研究成果報告書, 研究代表者：村本, 健一郎, 金沢大学自然科学研究科教

Enhanced light harvesting in bulk heterojunction photovoltaic devices with shape-controlled Ag nanomaterials: Ag nanoparticles versus Ag nanoplates

Enhanced power conversion efficiency (PCE(%)) with improved optical path length from two types of shape controlled silver (Ag) materials (Ag nanoplates versus Ag nanoparticles (NPs)) was studied in poly(3-hexylthiophene) (P3HT)/[6,6]-phenyl C 71 butyric acid methyl-ester (PC 71BM) or poly[N-9′′-hepta-decanyl-2,7-carbazole-alt-5,5-(4′, 7′-di-2-thienyl-2′,1′,3′-benzothiadiazole)] (PCDTBT)/[6,6]-phenyl C 71 butyric acid methyl-ester (PC 71BM) bulk heterojunction (BHJ) devices. The Ag nanoplates and Ag NPs can be synthesized by simple solution polyol chemistry with well defined size and shape. A BHJ with a 0.5 wt% optimized blend ratio of Ag nanoplates shows improved cell performance and photo-current density than a BHJ with Ag NPs owing to the enhanced light absorption with the results of an excitation of localized surface plasmon and efficient light scattering by the Ag nanoplates embedded BHJ film. When the BHJ is combined with the Ag nanoplates at an optimized ratio of 0.5 wt%, the PCE (%) increases from 3.2% to 4.4% in P3HT/PC 71BM, and from 5.9% to 6.6% in PCDTBT/PC 71BM BHJ devices. © 2012 The Royal Society of Chemistry.1

Photoluminescence from Si nanocrystals exposed to a hydrogen plasma

Si nanocrystals embedded in SiO₂films were exposed to an atomic H plasma at different temperatures. Photoluminescence intensity from the nanocrystals increases with increasing exposure time, followed by saturation that depends on the exposure temperature. The saturation level depends on the final exposure temperature and shows no dependence on the thermal history of exposure. This behavior is shown to be consistent with a model in which the steady-state passivation level is determined by a balance between defect passivation and depassivation by H, with the activation energy for the passivationreaction being larger than that for the depassivation reaction.This work was supported by Research Institute for Basic Science at Kangwon National University

Enzyme immunoassay for the rapid detection of Escherichia coli O157

An enzyme immunoassay(EIA) to detect Escherichia(E.) coli 0157 in pork was developed by using a sandwich-type assay on the 96-well microplates. All E. coli O157 strains and Citrobacter amalonaticus reacted strongly, however 29 E. coli serotypes and 15 non-E. coli bacterial strains showed negative in E. coli O157 EIA. E. coli 0157 in pork could be detected with in 13 h including 10 h in enrichment broth and 3 h in EIA. As few as 1.8 CFU of E. coli O157 per g of pork could be detected after enrichment, whereas above 1.8 \u3e. 1 o5 CFU of E. coli O157 per g of pork could be detected without enrichment. The E. coli 0157 EIA was a sensitive, easy-to-perform and efficient method for the screening of E. coliO 157 in pork