Accident and Speed Studies in Construction Zones

DOT-FH-11-8121The report includes results of two studies of construction zone traffic control. The first study involved analysis of traffic accidents occurring in 79 zones in seven states. Results indicate a before to during accident rate increase of 6.8%. Results also include breakdowns by accident types, severity, light conditions, roadway type, area type, work area roadway type, construction type and state. The second study was field testing of speed reduction methods. Speeds, erratic maneuvers and conflicts were measured at three sites--an urban freeway, a rural freeway, and an urban street. The field studies examined the effect of the following construction zone parameters on vehicle speeds and safety: Sequential flashing arrow boards; speed zoning (advisory and regulatory); enforcement; transverse striping; obliteration of nonappropriate pavement markings; taper length; lane width reduction; and active warning of speed zoning. Recommended guidelines for construction zone traffic controls are also included

Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids

Objective: There is currently a gap in on-site drug of abuse monitoring. Current detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. While remote laboratories then may provide confirmation and quantitative assessment of a presumptive positive, this instrumentation is expensive and decoupled from the initial sampling making the current drug-screening program inefficient and costly. The authors applied a noninvasive oral fluid sampling approach integrated with the in-development chip-based Programmable bio-nano-chip (p-BNC) platform for the detection of drugs of abuse. Method: The p-BNC assay methodology was applied for the detection of tetrahydrocannabinol, morphine, amphetamine, methamphetamine, cocaine, methadone and benzodiazepines, initially using spiked buffered samples and, ultimately, using oral fluid specimen collected from consented volunteers. Results: Rapid (∼10 min), sensitive detection (∼ng/mL) and quantitation of 12 drugs of abuse was demonstrated on the p-BNC platform. Furthermore, the system provided visibility to time-course of select drug and metabolite profiles in oral fluids; for the drug cocaine, three regions of slope were observed that, when combined with concentration measurements from this and prior impairment studies, information about cocaine-induced impairment may be revealed. Conclusions: This chip-based p-BNC detection modality has significant potential to be used in the future by law enforcement officers for roadside drug testing and to serve a variety of other settings, including outpatient and inpatient drug rehabilitation centers, emergency rooms, prisons, schools, and in the workplace

'Education, education, education' : legal, moral and clinical

This article brings together Professor Donald Nicolson's intellectual interest in professional legal ethics and his long-standing involvement with law clinics both as an advisor at the University of Cape Town and Director of the University of Bristol Law Clinic and the University of Strathclyde Law Clinic. In this article he looks at how legal education may help start this process of character development, arguing that the best means is through student involvement in voluntary law clinics. And here he builds upon his recent article which argues for voluntary, community service oriented law clinics over those which emphasise the education of students

Predicting new molecular targets for known drugs

Although drugs are intended to be selective, at least some bind to several physiological targets, explaining side effects and efficacy. Because many drug–target combinations exist, it would be useful to explore possible interactions computationally. Here we compared 3,665 US Food and Drug Administration (FDA)-approved and investigational drugs against hundreds of targets, defining each target by its ligands. Chemical similarities between drugs and ligand sets predicted thousands of unanticipated associations. Thirty were tested experimentally, including the antagonism of the β1 receptor by the transporter inhibitor Prozac, the inhibition of the 5-hydroxytryptamine (5-HT) transporter by the ion channel drug Vadilex, and antagonism of the histamine H4 receptor by the enzyme inhibitor Rescriptor. Overall, 23 new drug–target associations were confirmed, five of which were potent (less than 100 nM). The physiological relevance of one, the drug N,N-dimethyltryptamine (DMT) on serotonergic receptors, was confirmed in a knockout mouse. The chemical similarity approach is systematic and comprehensive, and may suggest side-effects and new indications for many drugs

Cardiomyocyte BRAF and type 1 RAF inhibitors promote cardiomyocyte and cardiac hypertrophy in mice in vivo

The extracellular signal-regulated kinase 1/2 (ERK1/2) cascade promotes cardiomyocyte hypertrophy and is cardioprotective, with the three RAF kinases forming a node for signal integration. Our aims were to determine if BRAF is relevant for human heart failure, whether BRAF promotes cardiomyocyte hypertrophy, and if Type 1 RAF inhibitors developed for cancer (that paradoxically activate ERK1/2 at low concentrations: the “RAF paradox”) may have the same effect. BRAF was upregulated in heart samples from patients with heart failure compared with normal controls. We assessed the effects of activated BRAF in the heart using mice with tamoxifen-activated Cre for cardiomyocyte-specific knock-in of the activating V600E mutation into the endogenous gene. We used echocardiography to measure cardiac dimensions/function. Cardiomyocyte BRAFV600E induced cardiac hypertrophy within 10 d, resulting in increased ejection fraction and fractional shortening over 6 weeks. This was associated with increased cardiomyocyte size without significant fibrosis, consistent with compensated hypertrophy. The experimental Type 1 RAF inhibitor, SB590885, and/or encorafenib (a RAF inhibitor used clinically) increased ERK1/2 phosphorylation in cardiomyocytes, and promoted hypertrophy, consistent with a “RAF paradox” effect. Both promoted cardiac hypertrophy in mouse hearts in vivo, with increased cardiomyocyte size and no overt fibrosis. In conclusion, BRAF potentially plays an important role in human failing hearts, activation of BRAF is sufficient to induce hypertrophy, and Type 1 RAF inhibitors promote hypertrophy via the “RAF paradox”. Cardiac hypertrophy resulting from these interventions was not associated with pathological features, suggesting that Type 1 RAF inhibitors may be useful to boost cardiomyocyte function

"It takes a safe place": Health and wellness at the Sierra Vista Food Co-op

In this thesis, I explore the context of a health food store/consumer food cooperative with an anthropological lens through integrating the meaning generated by shoppers with larger structural processes in the United States. To accomplish this, I illustrate research I conducted in an applied internship at the Sierra Vista Food Co-op in Sierra Vista, Arizona, where I executed a multi-faceted qualitative research design that informed a market study for the Sierra Vista Food Co-op organization and also the data for this thesis. In my analysis of the data I collected throughout the Sierra Vista Food Co-op internship, I uncovered a grouping of three narrative-based concepts in shopper’s descriptions of health and wellness in relation to the store and the USDA Organic labeled products housed within, which I refer to as trust, mistrust, and information seeking. In the chapters of this thesis, I outline the themes of trust, mistrust, and information seeking for Sierra Vista Food Co-op shoppers in detail through analytical descriptions coupled with quotes from shoppers. I then illustrate a theoretical framework through which I argue that USDA Organic policy and the subsequent USDA Organic label serves to commodify consumer sentiments of trust and mistrust in the larger health food market of the United States. I frame the commodification of consumer sentiments of trust and mistrust with the concepts of bio and disciplinary power, discourse, and the body through utilizing the perspective of poststructuralism and the work of Michel Foucault (1978). Through this theoretical framework, I also demonstrate how the theme of information seeking stands as an example of a process in which Sierra Vista Food Co-op shoppers actively constitute health and wellness modalities and ultimately forms of being in the world