Loss of Bid does not alter the cytokine and chemokine milieu of the joint

<p><b>Copyright information:</b></p><p>Taken from "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis"</p><p>http://arthritis-research.com/content/9/3/R49</p><p>Arthritis Research & Therapy 2007;9(3):R49-R49.</p><p>Published online 17 May 2007</p><p>PMCID:PMC2206343.</p><p></p> Pro-inflammatory cytokine production in ankle joints following transfer of K/BxN serum. Untreated wild-type (Wt) and Bid-/- mice were euthanized at three, five, or seven days post-serum transfer. Ankles from each mouse (days 3, = 6 (Wt) and n= 8 (Bid-/-); day 5, = 10; day 7, = 12 (Wt) and = 8 (Bid-/-)) were isolated, snap frozen, ground into a fine powder, lysed, and examined for production of tumor necrosis factor (TNF)α and IL-1β using sandwich ELISAs. Chemokine production in ankle joints following transfer of K/BxN serum. Ankles lysates as described above were examined for production of CXC chemokine (KC) and monocyte chemoattractant protein (MCP)-1 using ELISA. Data are shown as μg/μl per joint. Values represent the mean ± standard error, which were compared by Student's -test

Histological scores of ankle sections from wild-type (Wt) and Bid-/- mice

<p><b>Copyright information:</b></p><p>Taken from "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis"</p><p>http://arthritis-research.com/content/9/3/R49</p><p>Arthritis Research & Therapy 2007;9(3):R49-R49.</p><p>Published online 17 May 2007</p><p>PMCID:PMC2206343.</p><p></p> Bid-/- mice have increased inflammation and joint destruction compared to Wt mice. Ankles isolated from mice (Wt, = 9; Bid-/- = 7) were prepared as described in Figure 2. Ankle sections were evaluated and scored by a pathologist blinded to the study as described in the Materials and methods section. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test. Increased numbers of lymphocytes and polymorphonuclear (PMNs) cells in inflamed Bid-/- joints. Ankles were prepared as described above. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test. Arthritic Bid-/- mice have more macrophages in the pannus and in the whole joint. Ankles were examined for F4/80 antigen as described in Materials and methods. The number of positive cells for F4/80 in pannus, synovial lining, and whole joint was determined by a pathologist blinded to the study. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test