Phylogenetic trees depicting relationships among nucleotide and protein sequences from Fad genes.

<p>(a) Maximum Likelihood tree of Fad nucleotide sequences. Bootstrap values are shown next to nodes, values under 75% not reported. Accession numbers: AtFad KR154727, <i>C</i>. <i>gigas</i> 1 CGI_10016476, <i>C</i>. <i>gigas</i> 2 CGI_10019765, <i>C</i>. <i>gigas</i> 3 XM_011415748, <i>A</i>. <i>californica</i> 1 XM_005096991, <i>A</i>. <i>californica</i> 2 XM_005093125, <i>A</i>. <i>californica</i> 3 XM_005090516, <i>A</i>. <i>californica</i> 4 XM_005090520, <i>L</i>. <i>gigantea</i> 1 XM_009052983, <i>L</i>. <i>gigantea</i> 2 XM_009051720, <i>L</i>. <i>gigantea</i> 3 XM_009046829, <i>C</i>. <i>nobilis</i> (delta-5) KJ598786, <i>H</i>. <i>discus</i> (delta-5) 1 GQ470626, <i>H</i>. <i>discus</i> (delta-5) 2 GQ466197, <i>O</i>. <i>vulgaris</i> (delta-5) JN120258, <i>S</i>. <i>officinalis</i> KP260645. Exon-intron structure for <i>L</i>. <i>gigantea</i> and <i>C</i>. <i>gigas</i> are presented as gene models with exons (red boxes) and introns (red lines) adjacent to the corresponding species. (b) Maximum Likelihood tree of Fad protein sequences. Bootstrap values are shown next to nodes, and values under 75% not reported. Accession numbers: <i>C</i>. <i>gigas</i> 1 EKC33620, <i>C</i>. <i>gigas</i> 2 EKC30965, <i>C</i>. <i>gigas</i> 3 XP_011414050, <i>A</i>. <i>californica</i> 1 XP_005097048, <i>A</i>. <i>californica</i> 2 XP_005093182, <i>A</i>. <i>californica</i> 3 XP_005090573, <i>A</i>. <i>californica</i> 4 XP_005090577, <i>L</i>. <i>gigantea</i> 1 XP_009051231, <i>L</i>. <i>gigantea</i> 2 XP_009049968, <i>L</i>. <i>gigantea</i> 3 XP_009045077, <i>C</i>. <i>nobilis</i> (delta-5) AIC34709, <i>H</i>. <i>discus</i> (delta-5) 1 ADK38580, <i>H</i>. <i>discus</i> (delta-5) 2 ADK12703, <i>O</i>. <i>vulgaris</i> (delta-5) AEK20864, <i>S</i>. <i>officinalis</i> AKE92955.</p

Assembly summary statistics, for <i>C</i>. <i>farreri</i>, <i>A</i>. <i>irradians</i> and <i>C</i>. <i>olivaceus</i> transcriptomes using Trinity <i>de novo</i> assembler.

<p>Assembly summary statistics, for <i>C</i>. <i>farreri</i>, <i>A</i>. <i>irradians</i> and <i>C</i>. <i>olivaceus</i> transcriptomes using Trinity <i>de novo</i> assembler.</p

Maximum Likelihood trees of Elovl nucleotide and protein sequences.

<p>(a) Phylogenetic tree of Elovl nucleotide sequences. Bootstrap values are shown next to nodes, and values under 75% not reported. Accession numbers: NmElovla KR154728, NmElovlb KR154729, <i>C</i>. <i>gigas</i> 1 CGI_10028198, <i>C</i>. <i>gigas</i> 2 CGI_10008431, <i>C</i>. <i>gigas</i> 3 CGI_10020977, <i>C</i>. <i>gigas</i> 4 CGI_10012627 <i>C</i>. <i>gigas</i> 5 CGI_10007566, <i>C</i>. <i>gigas</i> 6 XM_011452473, <i>C</i>. <i>gigas</i> 7 XM_011452475, <i>A</i>. <i>californica</i> 1 XM_005098245, <i>A</i>. <i>californica</i> 2 XM_005095626, <i>A</i>. <i>californica</i> 3 XM_005106603, <i>L</i>. <i>gigantea</i> 1 XM_009047472, <i>L</i>. <i>gigantea</i> 2 XM_009052848, <i>C</i>. <i>nobilis</i> (<i>Elovl2/5</i>) KF245423, <i>O</i>. <i>vulgaris</i> (<i>Elovl4</i>) KJ590963, <i>O</i>. <i>vulgaris</i> (<i>Elovl2/5</i>) JX020803, <i>S</i>. <i>officinalis KP260646</i>. Exon-intron structure for <i>L</i>. <i>gigantea</i> and <i>C</i>. <i>gigas</i> are presented as gene models with exons (red boxes) and introns (red lines) adjacent to the corresponding species. (b) Phylogenetic tree of Elovl protein sequences. Bootstrap values shown next to nodes, and values under 75% not reported. Accession numbers: <i>C</i>. <i>gigas</i> 1 CGI_10028198, <i>C</i>. <i>gigas</i> 2 EKC41251, <i>C</i>. <i>gigas</i> 3 EKC25061, <i>C</i>. <i>gigas</i> 4 EKC39214, <i>C</i>. <i>gigas</i> 5 EKC19804, <i>C</i>. <i>gigas</i> 6 XP_011450775, <i>C</i>. <i>gigas</i> 7 XP_011450777, <i>A</i>. <i>californica</i> 1 XP_005098302, <i>A</i>. <i>californica</i> 2 XP_005095683, <i>A</i>. <i>californica</i> 3 XP_005106660, <i>L</i>. <i>gigantea</i> 1 XP_009045720, <i>L</i>. <i>gigantea</i> 2 XP_009051096, <i>C</i>. <i>nobilis</i> (Elovl2/5) AGW22128, <i>O</i>. <i>vulgaris</i> (Elovl4) AIA58679, <i>O</i>. <i>vulgaris</i> (Elovl2/5) AFM93779, <i>S</i>. <i>officinalis</i> AKE92956.</p

Fad and Elovl genes extracted from the currently available complete mollusc genomes of <i>C</i>. <i>gigas</i>, <i>A</i>. <i>californica</i> and <i>L</i>. <i>gigantea</i>.

<p>Fad and Elovl genes extracted from the currently available complete mollusc genomes of <i>C</i>. <i>gigas</i>, <i>A</i>. <i>californica</i> and <i>L</i>. <i>gigantea</i>.</p

List of candidate genes that encode putative Fad and Elovl proteins identified from <i>A</i>. <i>trapezia</i>, <i>N</i>. <i>albicilla</i> and <i>N</i>. <i>melanotragus</i> transcriptome assemblies.

<p>List of candidate genes that encode putative Fad and Elovl proteins identified from <i>A</i>. <i>trapezia</i>, <i>N</i>. <i>albicilla</i> and <i>N</i>. <i>melanotragus</i> transcriptome assemblies.</p

Alignment of Fad protein sequences showing conserved and variable regions.

<p>The haeme binding domain (HPGG) and the three histidine boxes (HXXXH, HXXHH and QXXHH) are indicated with a black rectangular outline.</p

Alignment of Elovl protein sequences showing conserved and variable regions.

<p>The histidine box (HXXHH) is indicated with a black rectangular outline. Highly conserved aa residues (K, E, DT, L, HH, N, H, MY, YY, T, LF, F) are designated with the symbol ★.</p

List of candidate genes that encode putative Fad and Elovl proteins identified from <i>L</i>. <i>pealeii</i> and <i>C</i>. <i>farreri</i> transcriptome assemblies.

<p>List of candidate genes that encode putative Fad and Elovl proteins identified from <i>L</i>. <i>pealeii</i> and <i>C</i>. <i>farreri</i> transcriptome assemblies.</p

List of mollusc Fad and Elovl genes, currently available in NCBI NR protein and UNIPROT databases.

<p>List of mollusc Fad and Elovl genes, currently available in NCBI NR protein and UNIPROT databases.</p