Construction of Shanghai Diabetes Clinical Database and real-world study

Objective·To construct a clinical database of diabetes in Shanghai, mine the value of clinical data, and carry out real-world study.Methods·The data were extracted from Shanghai Link Healthcare Database. All original clinical data have undergone standard processes such as desensitization, encryption, cleaning, standardization, information extraction and structuring, and clinical data were analyzed by the method of medical statistics or machine learning according to different research contents.Results·The database has imported the clinical data of 150 million visits and treatment records of 2.12 million diabetic patients in 37 municipal hospitals over a ten-year period from 2013 to 2022. The overall analysis showed the basic characteristics and development trends of all aspects of diabetes disease in real-world settings, the potential risks of diabetes are discovered by constructing retrospective cohort, and the inherent patterns of the disease are revealed by using machine learning methods such as cluster analysis and network analysis.Conclusion·The establishment of Shanghai Diabetes Clinical Database can not only summarize and show the clinical status of diabetes, but also obtain more scientific achievements with realistic clinical value by real-world clinical data study

Distinctive nuclear zone for RAD51-mediated homologous recombinational DNA repair

Genome-based functions are inseparable from the dynamic higher-order architecture of the cell nucleus. In this context, the repair of DNA damage is coordinated by precise spatiotemporal controls that target and regulate the repair machinery required to maintain genome integrity. However, the mechanisms that pair damaged DNA with intact template for repair by homologous recombination (HR) without illegitimate recombination remain unclear. This report highlights the intimate relationship between nuclear architecture and HR in mammalian cells. RAD51, the key recombinase of HR, forms spherical foci in S/G2 phases spontaneously. Using super-resolution microscopy, we show that following induction of DNA double-strand breaks RAD51 foci at damaged sites elongate to bridge between intact and damaged sister chromatids; this assembly occurs within bundle-shaped distinctive nuclear zones, requires interactions of RAD51 with various factors, and precedes ATP-dependent events involved the recombination of intact and damaged DNA. We observed a time-dependent transfer of single-stranded DNA overhangs, generated during HR, into such zones. Our observations suggest that RAD51-mediated homologous pairing during HR takes place within the distinctive nuclear zones to execute appropriate recombination

ATM Modulates the Loading of Recombination Proteins onto a Chromosomal Translocation Breakpoint Hotspot

Chromosome translocations induced by DNA damaging agents, such as ionizing radiation and certain chemotherapies, alter genetic information resulting in malignant transformation. Abrogation or loss of the ataxia-telangiectasia mutated (ATM) protein, a DNA damage signaling regulator, increases the incidence of chromosome translocations. However, how ATM protects cells from chromosome translocations is still unclear. Chromosome translocations involving the MLL gene on 11q23 are the most frequent chromosome abnormalities in secondary leukemias associated with chemotherapy employing etoposide, a topoisomerase II poison. Here we show that ATM deficiency results in the excessive binding of the DNA recombination protein RAD51 at the translocation breakpoint hotspot of 11q23 chromosome translocation after etoposide exposure. Binding of Replication protein A (RPA) and the chromatin remodeler INO80, which facilitate RAD51 loading on damaged DNA, to the hotspot were also increased by ATM deficiency. Thus, in addition to activating DNA damage signaling, ATM may avert chromosome translocations by preventing excessive loading of recombinational repair proteins onto translocation breakpoint hotspots

Broadband Wireless Channel in Composite High-Speed Railway Scenario: Measurements, Simulation, and Analysis

The rapid development of high-speed railway (HSR) and train-ground communications with high reliability, safety, and capacity promotes the evolution of railway dedicated mobile communication systems from Global System for Mobile Communications-Railway (GSM-R) to Long Term Evolution-Railway (LTE-R). The main challenges for LTE-R network planning are the rapidly time-varying channel and high mobility, because HSR lines consist of a variety of complex terrains, especially the composite scenarios where tunnels, cuttings, and viaducts are connected together within a short distance. Existing researches mainly focus on the path loss and delay spread for the individual HSR scenarios. In this paper, the broadband measurements are performed using a channel sounder at 950 MHz and 2150 MHz in a typical HSR composite scenario. Based on the measurements, the pivotal characteristics are analyzed for path loss exponent, power delay profile, and tap delay line model. Then, the deterministic channel model in which the 3D ray-tracing algorithm is applied in the composite scenario is presented and validated by the measurement data. Based on the ray-tracing simulations, statistical analysis of channel characteristics in delay and Doppler domain is carried out for the HSR composite scenario. The research results can be useful for radio interface design and optimization of LTE-R system

Bach1 Deficiency and Accompanying Overexpression of Heme Oxygenase-1 Do Not Influence Aging or Tumorigenesis in Mice

Oxidative stress contributes to both aging and tumorigenesis. The transcription factor Bach1, a regulator of oxidative stress response, augments oxidative stress by repressing the expression of heme oxygenase-1 (HO-1) gene (Hmox1) and suppresses oxidative stress-induced cellular senescence by restricting the p53 transcriptional activity. Here we investigated the lifelong effects of Bach1 deficiency on mice. Bach1-deficient mice showed longevity similar to wild-type mice. Although HO-1 was upregulated in the cells of Bach1-deficient animals, the levels of ROS in Bach1-deficient HSCs were comparable to those in wild-type cells. Bach1−/−; p53−/− mice succumbed to spontaneous cancers as frequently as p53-deficient mice. Bach1 deficiency significantly altered transcriptome in the liver of the young mice, which surprisingly became similar to that of wild-type mice during the course of aging. The transcriptome adaptation to Bach1 deficiency may reflect how oxidative stress response is tuned upon genetic and environmental perturbations. We concluded that Bach1 deficiency and accompanying overexpression of HO-1 did not influence aging or p53 deficiency-driven tumorigenesis. Our results suggest that it is useful to target Bach1 for acute injury responses without inducing any apparent deteriorative effect

Pelvic Height Planning versus Conventional Templating in preoperative planning of acetabulum cup size for THA

This study aimed to compare the accuracy of pelvic height planning vs. conventional templating for acetabulum cup. Total 200 consecutive patients underwent primary total hip arthroplasty (THA) were randomly grouped into: group A accepting conventional templating and group B accepting pelvic height planning. Preoperative measurement of acetabular cup was performed with conventional templating and pelvic height planning respectively. There were 57 cases in same size or with one type size discrepancy, 49 with two type size discrepancy, and 14 with three type size discrepancy in group A. There were 145 cases in same size or one type size discrepancy, 20 with two type size discrepancy, and 3 type size discrepancy in group B. The mean difference between the planned size and actual cup was 2.58 ± 0.89 mm vs. 1.38 ± 1.22 mm. The pelvic height planning is reliable to use for preoperative planning when compared with conventional templating