Comprehensive Analysis of Jumonji Domain C Family from <i>Citrus grandis</i> and Expression Profilings in the Exocarps of “Huajuhong” (<i>Citrus grandis</i> “Tomentosa”) during Various Development Stages

Citrus grandis “Tomentosa” (“Huajuhong”) is a famous Traditional Chinese Medicine. In this study, a total of 18 jumonji C (JMJC) domain-containing proteins were identified from C. grandis. The 18 CgJMJCs were unevenly located on six chromosomes of C. grandis. Phylogenetic analysis revealed that they could be classified into five groups, namely KDM3, KDM4, KDM5, JMJC, and JMJD6. The domain structures and motif architectures in the five groups were diversified. Cis-acting elements on the promoters of 18 CgJMJC genes were also investigated, and the abscisic acid-responsive element (ABRE) was distributed on 15 CgJMJC genes. Furthermore, the expression profiles of 18 CgJMJCs members in the exocarps of three varieties of “Huajuhong”, for different developmental stages, were examined. The results were validated by quantitative real-time PCR (qRT-PCR). The present study provides a comprehensive characterization of JMJC domain-containing proteins in C. grandis and their expression patterns in the exocarps of C. grandis “Tomentosa” for three varieties with various development stages

Comprehensive Analysis of N6-Methyladenosine Regulatory Genes from <i>Citrus grandis</i> and Expression Profilings in the Fruits of “Huajuhong” (<i>C. grandis</i> “Tomentosa”) during Various Development Stages

Citrus grandis “Tomentosa” (“Huajuhong”) is a famous traditional Chinese medicine. The aim of the present study is to provide a comprehensive characterization of the m6A regulatory genes from C. grandis, and examine their expression patterns in fruits of C. grandis “Tomentosa” during various developmental stages. A total of 26 N6-methyladenosine (m6A) regulatory proteins were identified from the genome of C. grandis, which were distributed across nine chromosomes in C. grandis. Phylogenetic relationships revealed that all m6A regulatory genes were divided into groups of m6A writers, erasers, and readers. The m6A writer groups included CgMTA, CgMTB, and CgMTC three MTs (methyltransferases), one CgVIR (virilizer), one CgHAKAI (E3 ubiquitin ligase HAKAI), and one CgFIP37 (FKBP interacting protein 37). Moreover, 10 CgALKBH (α-ketoglutarate-dependent dioxygenase homolog) members (numbered from CgALKBH1 to CgALKBH10) and 10 CgECT (C-terminal region) members (numbered from CgECT1 to CgECT10) in C. grandis were identified as m6A erasers and readers, respectively. The domain structures and motif architectures among the groups of m6A writers, erasers, and readers were diverse. Cis-acting elements in the promoters of the 26 m6A regulatory genes predicted that the abscisic acid-responsive (ABA) element (ABRE) was present on the promoters of 19 genes. In addition, the expression profiles of all m6A regulatory genes were examined in the fruits of two varieties of C. grandis “Tomentosa” during different growth stages to give basic hints for further investigation of the function of the N6-methyladenosine regulatory genes in C. grandis “Tomentosa”

Comprehensive Analysis of Jumonji Domain C Family from Citrus grandis and Expression Profilings in the Exocarps of &ldquo;Huajuhong&rdquo; (Citrus grandis &ldquo;Tomentosa&rdquo;) during Various Development Stages

Citrus grandis &ldquo;Tomentosa&rdquo; (&ldquo;Huajuhong&rdquo;) is a famous Traditional Chinese Medicine. In this study, a total of 18 jumonji C (JMJC) domain-containing proteins were identified from C. grandis. The 18 CgJMJCs were unevenly located on six chromosomes of C. grandis. Phylogenetic analysis revealed that they could be classified into five groups, namely KDM3, KDM4, KDM5, JMJC, and JMJD6. The domain structures and motif architectures in the five groups were diversified. Cis-acting elements on the promoters of 18 CgJMJC genes were also investigated, and the abscisic acid-responsive element (ABRE) was distributed on 15 CgJMJC genes. Furthermore, the expression profiles of 18 CgJMJCs members in the exocarps of three varieties of &ldquo;Huajuhong&rdquo;, for different developmental stages, were examined. The results were validated by quantitative real-time PCR (qRT-PCR). The present study provides a comprehensive characterization of JMJC domain-containing proteins in C. grandis and their expression patterns in the exocarps of C. grandis &ldquo;Tomentosa&rdquo; for three varieties with various development stages

Comprehensive Analysis of N6-Methyladenosine Regulatory Genes from Citrus grandis and Expression Profilings in the Fruits of &ldquo;Huajuhong&rdquo; (C. grandis &ldquo;Tomentosa&rdquo;) during Various Development Stages

Citrus grandis &ldquo;Tomentosa&rdquo; (&ldquo;Huajuhong&rdquo;) is a famous traditional Chinese medicine. The aim of the present study is to provide a comprehensive characterization of the m6A regulatory genes from C. grandis, and examine their expression patterns in fruits of C. grandis &ldquo;Tomentosa&rdquo; during various developmental stages. A total of 26 N6-methyladenosine (m6A) regulatory proteins were identified from the genome of C. grandis, which were distributed across nine chromosomes in C. grandis. Phylogenetic relationships revealed that all m6A regulatory genes were divided into groups of m6A writers, erasers, and readers. The m6A writer groups included CgMTA, CgMTB, and CgMTC three MTs (methyltransferases), one CgVIR (virilizer), one CgHAKAI (E3 ubiquitin ligase HAKAI), and one CgFIP37 (FKBP interacting protein 37). Moreover, 10 CgALKBH (&alpha;-ketoglutarate-dependent dioxygenase homolog) members (numbered from CgALKBH1 to CgALKBH10) and 10 CgECT (C-terminal region) members (numbered from CgECT1 to CgECT10) in C. grandis were identified as m6A erasers and readers, respectively. The domain structures and motif architectures among the groups of m6A writers, erasers, and readers were diverse. Cis-acting elements in the promoters of the 26 m6A regulatory genes predicted that the abscisic acid-responsive (ABA) element (ABRE) was present on the promoters of 19 genes. In addition, the expression profiles of all m6A regulatory genes were examined in the fruits of two varieties of C. grandis &ldquo;Tomentosa&rdquo; during different growth stages to give basic hints for further investigation of the function of the N6-methyladenosine regulatory genes in C. grandis &ldquo;Tomentosa&rdquo;

an efficient group-based secret sharing scheme

We propose a new secret sharing scheme which can be computed over an Abelian group, such as (Binary string, XOR) and (Integer, Addition). Therefore, only the XOR or the addition operations are required to implement the scheme. It is very efficient and fits for low-cost low-energy applications such as RFID tags. Making shares has a geometric presentation which makes our scheme be easily understood and analyzed. &copy; 2011 Springer-Verlag Berlin Heidelberg

efficient secret sharing schemes

We propose a new XOR-based (k,n) threshold secret SSS, where the secret is a binary string and only XOR operations are used to make shares and recover the secret. Moreover, it is easy to extend our scheme to a multi-secret sharing scheme. When k is closer to n, the computation costs are much lower than existing XOR-based schemes in both distribution and recovery phases. In our scheme, using more shares (&ge; k) will accelerate the recovery speed. &copy; 2011 Springer-Verlag

Hydrated eutectic electrolyte with ligand-oriented solvation shell to boost the stability of zinc battery

An electrolyte cation additive strategy provides a versatile route for developing high-energy and long-life aqueous zinc-ion hybrid capacitors. However, the mechanisms of energy storage and Zn anode protection are still unclear in Zn-based systems with dual-ion electrolytes. Here, a dual charge storage mechanism for zinc-ion hybrid capacitors with both cations and anions adsorption/desorption and the reversible formation of Zn4SO4(OH)6·xH2O enabled by the Mg2+ additive in the common aqueous ZnSO4 electrolyte are proposed. Theoretical calculations verify that the self-healing electrostatic shield effect and the solvation-sheath structure regulation rendered by the Mg2+ additive account for the observed uniform Zn deposition and dendrite suppression. As a result, an additional energy storage capacity of ≈50% compared to that in a pure 2 m ZnSO4 electrolyte and an extended cycle life with capacity retention of 98.7% after 10 000 cycles are achieved. This work highlights the effectiveness of electrolyte design for dual-ion carrier storage mechanism in aqueous devices toward high energy density and long cycle life.Ministry of Education (MOE)Submitted/Accepted versionThis work was supported by the National Natural Science Foundation of China (Grant Nos. 51972346, 51932011), the Hunan Natural Science Fund for Distinguished Young Scholar (2021JJ1006), the Program of Youth Talent Support for Hunan Province (2020RC3011), the Innovation-Driven Project of Central South University (No. 2020CX024), and the Fundamental Research Funds for the Central Universities of Central South University (No. 202321024). H.J.F acknowledges the financial support from Ministry of Education by Tier 1 grant (RG85/20)