Fabrication of WO3·2H2O/BC Hybrids by the Radiation Method for Enhanced Performance Supercapacitors

In this study, we described a facile process for the fabrication of tungsten oxide dihydrate/bamboo charcoal hybrids (WO3·2H2O/BC) by the γ-irradiation method. The structural, morphological, and electrochemical properties of WO3·2H2O/BC hybrids were investigated using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), cyclic voltammetry (CV), galvanostatic charge/discharge (GCD), and electrochemical impedance spectroscopy (EIS) techniques. The combination of BC (electrical double layer charge) and WO3·2H2O (pseudocapacitance) created a combined effect, which enhanced the specific capacitance and superior cyclic stability of the WO3·2H2O/BC hybrid electrode. The WO3·2H2O/BC hybrids showed the higher specific capacitance (391 F g−1 at 0.5 A g−1 over the voltage range from −1 to 0 V), compared with BC (108 F g−1) in 6 M KOH solution. Furthermore, the hybrid electrode showed superior long-term performance with 82% capacitance retention even after 10,000 cycles. The experimental results demonstrated that the high performance of WO3·2H2O/BC hybrids could be a potential electrode material for supercapacitors

18F-FDG PET/CT findings in a patient with blastic plasmacytoid dendritic cell neoplasm and post-transplant lymphoproliferative disorder after hematopoietic stem cell transplantation: a case report

BackgroundBlastic plasmacytoid dendritic cell neoplasm (BPDCN) is an extremely rare hematopoietic malignancy, which originating from precursors of plasmacytoid dendritic cells. Allogeneic hematopoietic stem cell transplantation (HSCT) is normally considered in the treatment of BPDCN patients to acquire sustained remission. Post-transplant lymphoproliferative disorder (PTLD) is a group of conditions involving abnormal lymphoid cells proliferation in the context of extrinsic immunosuppression after solid organ transplantation (SOT) or HSCT. Herein, we report a patient with BPDCN, who suffered from PTLD after allogeneic HSCT.Case presentationA 66-year-old man was diagnosed with BPDCN, confirmed by pathologic examination after splenectomy. The post-surgery 18F-fluoro-2-deoxy-D-glucose-positron emission tomography/computed tomography (18F-FDG PET/CT) showed multifocal 18F-FDG avidity in the left cheek, lymph nodes and bone marrow. The patient started chemotherapy, followed by allogeneic HSCT and immunosuppressive therapy. Four months after the HSCT, the patient developed intermittent fever and recurrent lymphadenopathy, accompanied with progressively elevated Epstein–Barr virus (EBV)-DNA both in serum and lymphocytes. 18F-FDG PET/CT was performed again and found multiple new enlarged 18F-FDG-avid lymph nodes, while the previous hypermetabolic lesions all disappeared. The pathology of mesenteric lymph node indicated a monomorphic PTLD (diffuse large B-cell lymphoma). Then the immunosuppressive medications were stopped and two cycles of Rituximab were given, and the follow-up CT scan indicated a complete response.ConclusionWhen patients with BPDCN recurred new enlarged lymph nodes after allogeneic HSCT and immunosuppressive therapy, PTLD should be taken into consideration. 18F-FDG PET/CT may provide additional evidence for supporting or refuting the suspicion of PTLD, and suggest lesions accessible for biopsy

Characterization of stemness features and construction of a stemness subtype classifier to predict survival and treatment responses in lung squamous cell carcinoma

Abstract Background Cancer stemness has been proven to affect tumorigenesis, metastasis, and drug resistance in various cancers, including lung squamous cell carcinoma (LUSC). We intended to develop a clinically applicable stemness subtype classifier that could assist physicians in predicting patient prognosis and treatment response. Methods This study collected RNA-seq data from TCGA and GEO databases to calculate transcriptional stemness indices (mRNAsi) using the one-class logistic regression machine learning algorithm. Unsupervised consensus clustering was conducted to identify a stemness-based classification. Immune infiltration analysis (ESTIMATE and ssGSEA algorithms) methods were used to investigate the immune infiltration status of different subtypes. Tumor Immune Dysfunction and Exclusion (TIDE) and Immunophenotype Score (IPS) were used to evaluate the immunotherapy response. The pRRophetic algorithm was used to estimate the efficiency of chemotherapeutic and targeted agents. Two machine learning algorithms (LASSO and RF) and multivariate logistic regression analysis were performed to construct a novel stemness-related classifier. Results We observed that patients in the high-mRNAsi group had a better prognosis than those in the low-mRNAsi group. Next, we identified 190 stemness-related differentially expressed genes (DEGs) that could categorize LUSC patients into two stemness subtypes. Patients in the stemness subtype B group with higher mRNAsi scores exhibited better overall survival (OS) than those in the stemness subtype A group. Immunotherapy prediction demonstrated that stemness subtype A has a better response to immune checkpoint inhibitors (ICIs). Furthermore, the drug response prediction indicated that stemness subtype A had a better response to chemotherapy but was more resistant to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs). Finally, we constructed a nine-gene-based classifier to predict patients’ stemness subtype and validated it in independent GEO validation sets. The expression levels of these genes were also validated in clinical tumor specimens. Conclusion The stemness-related classifier could serve as a potential prognostic and treatment predictor and assist physicians in selecting effective treatment strategies for patients with LUSC in clinical practice

PZT-Based Detection of Compactness of Concrete in Concrete Filled Steel Tube Using Time Reversal Method

A smart aggregate-based approach is proposed for the concrete compactness detection of concrete filled steel tube (CFST) columns. The piezoceramic-based smart aggregates (SAs) were embedded in the predetermined locations prior to the casting of concrete columns to establish a wave-based smart sensing system for the concrete compactness detection purpose. To evaluate the efficiency of the developed approach, six specimens of the CFST columns with the rectangular cross-section were produced by placing some artificial defects during casting of concrete for simulating various uncompacted voids such as cavities, cracks, and debond. During the test, the time reversal technology was applied to rebuild the received signals and launch the reversed signals again by SAs, to overcome the issue of the lack of the prototype. Based on the proposed nonprototype, two indices of time reversibility (TR) and symmetry (SYM) were applied to relatively evaluate the level of concrete compactness in the range of the two SAs. The experimental results show that the developed method can effectively detect the compactness of concrete in CFST columns

STUDY ON THE COMPILATION OF TYPICAL EXCITATION SPECTRUM OF SUSPENSION DYNAMIC K＆C TEST

The traditional quasi-static K＆C characteristic test can not reflect the hysteresis effect caused by shock absorber damping and bushing viscoelasticity comprehensively and effectively, but the suspension dynamic K＆C characteristic test can make up the deficiency of the quasi-static characteristic test.Development of dynamic K＆C test involves two aspects of excitation spectrum and performance evaluation of the research content, in this paper, A class A sedan for the study of the carrier, through A skid pad road load spectrum sampling test and simulation analysis, through the bench test iterations, designed the suspension K＆C dynamic characteristic test of typical excitation spectrum, and its validity is verified by bench test and virtual test.The results show that the dynamic characteristics of the suspension can be reflected effectively by using the typical excitation spectrum designed in this paper, which provides some support for the research of wheel positioning parameters and the development of K＆C test technology

Decomposition of macroalgal blooms influences phosphorus release from the sediments and implications for coastal restoration in Swan Lake, Shandong, China

Green tides (macroalgal blooms) have occurred worldwide frequently in coastal areas during the last few decades, which dramatically modify nutrient recycling in water column and the sediments. Rongcheng Swan Lake is a shallow coastal lagoon in eastern Shandong Peninsula, China, suffering from extensive macroalgal blooms dominated by Chaetomorpha spp. during the early summer and autumn. In the present study, the sediments collected from different lake regions and filamentous Chaetomorpha linum were incubated in the laboratory to investigate the influence of macroalgal decomposition on phosphorus (P) release from the sediments. In additon, some water quality parameters, including dissolved oxygen (DO), chemical oxygen demand (COD) and pH, were monitored during the 30-day experiment. Due to the Chaetomorpha decomposition, DO concentration and pH value in overlying water declined greatly. DO concentrations in sediment + 30 g algae + water treatment were very low (< 0.15 mg L-1), which remained anaerobic throughout the incubation. In addition, COD concentration in the treatments with algae increased dramatically and the maximum varied between 20.07 and 69.26 mg L-1. In general, with the addition of greater amount of decomposing algae, a more visible change in each parameter was observed. During the decomposition process, SRP concentration in overlying water increased significantly, with the maximum of 1.51 mg L-1. At the two studied regions, SRP concentrations in sediment + 30 g algae + water were significantly higher than those in sediment + water and algae + water treatments, respectively (p < 0.05). The remarkable increase in P level in overlying water in sediment + algae + water treatment was mainly due to the P release from decomposing macroalgae during the initial 5 days of incubation and from the sediments at late stage, respectively. The decomposition of large qualities of Chaetomorpha promoted sediment P release obviously through changing environmental conditions, whose influence effect differed between the macroalgal dominated and non-dominated zone. In the macroalgal bloom zone, P release amount was high and the P released from sediments constituted the most part of P increase in overlying water. Our results indicate that the influence of Chaetomorpha blooms on sediment P release can not be neglected during the degradation phase in Swan Lake. (C) 2013 Elsevier B. V. All rights reserved

Glutaric Acid-Mediated Apoptosis in Primary Striatal Neurons

Glutaric acid (GA) has been implicated in the mechanism of neurodegeneration in glutaric aciduria type I. In the present study, the potential cytotoxic effects of GA (0.1~50 mM for 24~96 h) were examined in cultured primary rat striatal neurons. Results showed increase in the number of cells labeled by annexin-V or with apoptotic features shown by Hoechst/PI staining and transmission electron microscopy (TEM) and upregulation of the expression of mRNA as well as the active protein fragments caspase 3, suggesting involvement of the caspase 3-dependent apoptotic pathway in GA-induced striatal neuronal death. This effect was in part suppressed by the N-methyl-D-aspartate (NMDA) receptor antagonist MK-801 but not the α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) antagonist 6-cyano-7-nitroquinoxalone-2,3-dione (CNQX). Thus, GA may trigger neuronal damage partially through apoptotic pathway and via activation of NMDA receptors in cultured primary striatal neurons

Identification and Verification of Core Genes in Colorectal Cancer

Colorectal cancer, a malignant neoplasm that occurs in the colorectal mucosa, is one of the most common types of gastrointestinal cancer. Colorectal cancer has been studied extensively, but the molecular mechanisms of this malignancy have not been characterized. This study identified and verified core genes associated with colorectal cancer using integrated bioinformatics analysis. Three gene expression profiles (GSE15781, GSE110223, and GSE110224) were downloaded from the Gene Expression Omnibus (GEO) databases. A total of 87 common differentially expressed genes (DEGs) among GSE15781, GSE110223, and GSE110224 were identified, including 19 upregulated genes and 68 downregulated genes. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed for common DEGs using clusterProfiler. These common DEGs were significantly involved in cancer-associated functions and signaling pathways. Then, we constructed protein-protein interaction networks of these common DEGs using Cytoscape software, which resulted in the identification of the following 10 core genes: SST, PYY, CXCL1, CXCL8, CXCL3, ZG16, AQP8, CLCA4, MS4A12, and GUCA2A. Analysis using qRT-PCR has shown that SST, CXCL8, and MS4A12 were significant differentially expressed between colorectal cancer tissues and normal colorectal tissues (P<0.05). Gene Expression Profiling Interactive Analysis (GEPIA) overall survival (OS) has shown that low expressions of AQP8, ZG16, CXCL3, and CXCL8 may predict poor survival outcome in colorectal cancer. In conclusion, the core genes identified in this study contributed to the understanding of the molecular mechanisms involved in colorectal cancer development and may be targets for early diagnosis, prevention, and treatment of colorectal cancer

“Guidezilla” extension catheter combined with balloon technique for treating pulmonary artery stenosis caused by Takayasu arteritis

Takayasu arteritis (TA) is a rare systemic vasculitis of the aorta and its primary branches, which usually occurs in young women. Due to its insidious onset and lack of specific symptoms, this disease can be easily misdiagnosed or missed. Approximately 50 ​% of the patients having TA with pulmonary artery involvement develop pulmonary hypertension (PH). The 3-year survival rate among patients with TA-related PH is lower than that among patients with TA alone. Early balloon pulmonary angioplasty (BPA) can improve the clinical symptoms and survival of patients with stable TA. To the best of our knowledge, this is the first case reported in the English literature in which a “Guidezilla” catheter was used during BPA to treat stenosis and occlusion of the pulmonary artery caused by Takayasu arteritis (TA)