Parameter Indentification of Wave Period by Superposition Method

This paper presents a study of the parameter identification of period by the superposition of wave in the actuator model using the finite element method. In this research, the period of superposed wave is identified so as to minimize the performance function. As the state equation, the Navier-Stokes equations are used for the analysis of flow in the actuator model. The quasi-linear approximation of advection velocity is given by the Adams-Bashforth formula which has second order accuracy. To solve the state equation, the implicit time integration is applied to the temporal discretization. The bubble function element using the stabilized bubble function method is utilized for the spatial discretization.【査読有

Quantitative analysis of major elements in igneous rocks with X-ray fluorescence spectrometer “ZSX primus II” using a 1:10 dilution glass bead

Detailed procedures of sample processing including preparation of a 1: 10 dilution glass bead and evaluations of calibration lines of the X-ray fluorescence spectrometer for major element compositions of igneous rock samples are presented. We used 11 igneous rock standard samples of the Geological Survey of Japan and the synthetic material for the calibration. A powdered rock sample ignited at 900 ° C for four hours and lithium tetraborate as an alkali flux ignited at 700 ° C for four hours are weighed 0.4000 ± 0.0001 g and 4.0000 ± 0.0001 g, respectively. The mixture of rock powder sample and lithium tetraborate is put into a platinum crucible and fused to a glass bead. The calibration lines for basalts and andesites named "Major12" analyze 10 major elements such as Si, Ti, Al, Fe, Mn, Mg, Ca, Na, K and P in 10 minutes. The result of repeated analyses of six standard materials shows that the relative standard deviations are less than 3% and relative errors are less than 1.2%. Therefore, the calibration lines "Major12" are sufficient to be applied to routine measurement of igneous rocks. For analysis of ultramafic rocks, another set of calibration lines "majorOl\u27\u27 was made based on standard samples including synthesized materials of SiO, and MgO reagents, and the calibration lines cover wider Si, Mg, Ni and Cr ranges than "Major12". The calibration lines "majorOl\u27\u27 successfully reproduced concentrations of nine major element compositions (Si, Ti, Al, Fe, Mn, Mg, Ca, Ni, Cr) of the standard samples of ultramafic rocks

Potential Application of the Myocardial Scintigraphy Agent [¹²³I]BMIPP in Colon Cancer Cell Imaging

[123I]β-methyl-p-iodophenyl-pentadecanoic acid ([123I]BMIPP), which is used for nuclear medicine imaging of myocardial fatty acid metabolism, accumulates in cancer cells. However, the mechanism of accumulation remains unknown. Therefore, this study aimed to elucidate the accumulation and accumulation mechanism of [123I]BMIPP in cancer cells. We compared the accumulation of [123I]BMIPP in cancer cells with that of [18F]FDG and found that [123I]BMIPP was a much higher accumulation than [18F]FDG. The accumulation of [123I]BMIPP was evaluated in the presence of sulfosuccinimidyl oleate (SSO), a CD36 inhibitor, and lipofermata, a fatty acid transport protein (FATP) inhibitor, under low-temperature conditions and in the presence of etomoxir, a carnitine palmitoyl transferase I (CPT1) inhibitor. The results showed that [123I]BMIPP accumulation was decreased in the presence of SSO and lipofermata in H441, LS180, and DLD-1 cells, suggesting that FATPs and CD36 are involved in [123I]BMIPP uptake in cancer cells. [123I]BMIPP accumulation in all cancer cell lines was significantly decreased at 4 °C compared to that at 37 °C and increased in the presence of etomoxir in all cancer cell lines, suggesting that the accumulation of [123I]BMIPP in cancer cells is metabolically dependent. In a biological distribution study conducted using tumor-bearing mice transplanted with LS180 cells, [123I]BMIPP highly accumulated in not only LS180 cells but also normal tissues and organs (including blood and muscle). The tumor-to-intestine or large intestine ratios of [123I]BMIPP were similar to those of [18F]FDG, and the tumor-to-large-intestine ratios exceeded 1.0 during 30 min after [123I]BMIPP administration in the in vivo study. [123I]BMIPP is taken up by cancer cells via CD36 and FATP and incorporated into mitochondria via CPT1. Therefore, [123I]BMIPP may be useful for imaging cancers with activated fatty acid metabolism, such as colon cancer. However, the development of novel imaging radiotracers based on the chemical structure analog of [123I]BMIPP is needed

X Chromosome Contribution to the Genetic Architecture of Primary Biliary Cholangitis.

BACKGROUND & AIMS: Genome-wide association studies in primary biliary cholangitis (PBC) have failed to find X chromosome (chrX) variants associated with the disease. Here, we specifically explore the chrX contribution to PBC, a sexually dimorphic complex autoimmune disease. METHODS: We performed a chrX-wide association study, including genotype data from 5 genome-wide association studies (from Italy, United Kingdom, Canada, China, and Japan; 5244 case patients and 11,875 control individuals). RESULTS: Single-marker association analyses found approximately 100 loci displaying P < 5 × 10(-4), with the most significant being a signal within the OTUD5 gene (rs3027490; P = 4.80 × 10(-6); odds ratio [OR], 1.39; 95% confidence interval [CI], 1.028-1.88; Japanese cohort). Although the transethnic meta-analysis evidenced only a suggestive signal (rs2239452, mapping within the PIM2 gene; OR, 1.17; 95% CI, 1.09-1.26; P = 9.93 × 10(-8)), the population-specific meta-analysis showed a genome-wide significant locus in East Asian individuals pointing to the same region (rs7059064, mapping within the GRIPAP1 gene; P = 6.2 × 10(-9); OR, 1.33; 95% CI, 1.21-1.46). Indeed, rs7059064 tags a unique linkage disequilibrium block including 7 genes: TIMM17B, PQBP1, PIM2, SLC35A2, OTUD5, KCND1, and GRIPAP1, as well as a superenhancer (GH0XJ048933 within OTUD5) targeting all these genes. GH0XJ048933 is also predicted to target FOXP3, the main T-regulatory cell lineage specification factor. Consistently, OTUD5 and FOXP3 RNA levels were up-regulated in PBC case patients (1.75- and 1.64-fold, respectively). CONCLUSIONS: This work represents the first comprehensive study, to our knowledge, of the chrX contribution to the genetics of an autoimmune liver disease and shows a novel PBC-related genome-wide significant locus.The article is available via Open Access. Click on the 'Additional link' above to access the full-text.Published version, accepted versio