Prevalence and Prognostic Significance of HPV in Laryngeal Squamous Cell Carcinoma in Northeast China

Background/Aims: Human papillomavirus (HPV) is an etiological risk factor for a subset of head and neck squamous cell carcinomas. HPV has been proven to be a powerful prognostic biomarker for oropharyngeal cancer, but its role in the larynx has not been explored in depth. Here, we sought to evaluate the prevalence and genotype distribution of HPV in patients with laryngeal squamous cell carcinoma (LSCC) in northeast China. Methods: HPV DNA in specimens from 211 patients diagnosed with LSCC was analyzed by the polymerase chain reaction and in situ hybridization, and p16 overexpression was evaluated by immunohistochemistry. p16 expression was scored positive if strong and diffuse nuclear and cytoplasmic staining was present in > 75% of tumor cells. Results: In this study, infection with HPV and p16 expression were not absolutely consistent. Among all patients, 132 (62.6%) were positive for HPV DNA (HPV+), while 23 (10.9%) were inconsistent for HPV and p16. Multivariate analysis indicated that HPV, but not p16, is an independent prognostic factor for overall survival in LSCC. Overall survival was significantly improved in HPV+ LSCC patients compared with the HPV-negative group (hazard ratio, 0.395; 95% confidence interval, 0.185–0.843; p = 0.016). Among the 132 HPV+ patients, 28 (21.2%) were HPV-16 single infection. Conclusion: This study indicates that HPV DNA is a more reliable surrogate marker than p16 for the prediction of survival in patients with LSCC

Quercetin Suppresses Cyclooxygenase-2 Expression and Angiogenesis through Inactivation of P300 Signaling

Quercetin, a polyphenolic bioflavonoid, possesses multiple pharmacological actions including anti-inflammatory and antitumor properties. However, the precise action mechanisms of quercetin remain unclear. Here, we reported the regulatory actions of quercetin on cyclooxygenase-2 (COX-2), an important mediator in inflammation and tumor promotion, and revealed the underlying mechanisms. Quercetin significantly suppressed COX-2 mRNA and protein expression and prostaglandin (PG) E(2) production, as well as COX-2 promoter activation in breast cancer cells. Quercetin also significantly inhibited COX-2-mediated angiogenesis in human endothelial cells in a dose-dependent manner. The in vitro streptavidin-agarose pulldown assay and in vivo chromatin immunoprecipitation assay showed that quercetin considerably inhibited the binding of the transactivators CREB2, C-Jun, C/EBPβ and NF-κB and blocked the recruitment of the coactivator p300 to COX-2 promoter. Moreover, quercetin effectively inhibited p300 histone acetyltransferase (HAT) activity, thereby attenuating the p300-mediated acetylation of NF-κB. Treatment of cells with p300 HAT inhibitor roscovitine was as effective as quercetin at inhibiting p300 HAT activity. Addition of quercetin to roscovitine-treated cells did not change the roscovitine-induced inhibition of p300 HAT activity. Conversely, gene delivery of constitutively active p300 significantly reversed the quercetin-mediated inhibition of endogenous HAT activity. These results indicate that quercetin suppresses COX-2 expression by inhibiting the p300 signaling and blocking the binding of multiple transactivators to COX-2 promoter. Our findings therefore reveal a novel mechanism of action of quercetin and suggest a potential use for quercetin in the treatment of COX-2-mediated diseases such as breast cancers

New Technology Innovation Model Centered on Enterprises

As the elderly care sector is characterized by several challenges, rethought and restructured organizations and institutions and thus innovation are necessary. This study investigated (1) what innovation in the elderly care sector means and (2) which factors determine the innovativeness of elderly care organizations. Our goal was to develop a framework to catch innovation and explain the capacity for innovation in the elderly care sector. Via case studies within five Flemish elderly care organizations, the applicability of the complex adaptive system (CAS) theory was examined. The results showed that organizations partially acted as CASs, but totally acting as CASs was restrained by top-down forces, procedures, and regulations

Three Dimensional Model of Technology Innovation System Centered on Enterprises

Although there is a high level of practitioner, policymaker, and scholar interest in social entrepreneurship, most research is based on case studies and success stories of successful social entrepreneurs in a single country. We develop a methodology to measure population-based social entrepreneurship activity (SEA) prevalence rates and test it in 49 countries. Our results provide insights into institutional and individual drivers of SEA. Using the Global Entrepreneurship Monitor (GEM) methodology of Total Entrepreneurial Activity (TEA), we find that countries with higher rates of traditional entrepreneurial activity also tend to have higher rates of social entrepreneurial activity. We develop a broad definition of social entrepreneurship and then explore types based on social mission, revenue model, and innovativeness

Attack‐resilient event‐triggered distributed control strategy of microgrid based on multi‐objective optimization

Abstract As an important form of renewable energy utilization, microgrid (MG) is considered to be the main bearing form of distributed generation in the future. One of the most concerning issues in the operation of MG is how to realize its economic dispatch (ED). Nowadays, distributed algorithms have been increasingly used to solve the economic dispatch problem of MG. However, the MG based on the distributed optimization architecture must bear higher cyber‐attack risks. To address this issue, this paper investigates the distributed robust ED problem of MG. Firstly, a multi‐objective dispatch model of MG using a linear weighted sum (LWS) algorithm is developed, which considers the environmental and economic costs. On this basis, an event‐triggered fully distributed algorithm is proposed, which can effectively reduce communication times. Furthermore, an attack resilient strategy against false data injection (FDI) attacks is implemented in the proposed fully distributed algorithm, which has strong robustness against various colluding attacks and non‐colluding attacks, and can eliminate incorrect measurement of incremental cost and power generation data. Finally, the effectiveness of the proposed distributed control strategy is demonstrated through case studies in this paper

Editorial: Statistical and computational methods for single-cell sequencing analysis

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Robust Optimization of Power Consumption for Public Buildings Considering Forecasting Uncertainty of Environmental Factors

In recent years, with the advancement of urban construction in China, the optimization of power consumption in public buildings has been focused on. The optimization of power consumption in public buildings is based on the prediction of natural illuminance, outdoor air temperature and flow of people in public building. Therefore, it is worthwhile to study how to formulate a power consumption strategy with consideration of forecasting uncertainty of environmental factors. The robust-index method is proposed to deal with the problem of forecasting uncertainty. Firstly, this paper establishes power consumption models for lighting systems, air-conditioning systems, and elevator systems in public buildings. Secondly, the robust indexes for each system and the synthetic robust index are established. Thirdly, the objective function is formulated to reduce the total electricity cost with the robust indexes applied as additional constraints to the optimization problem, therefore the obtained power consumption schedules are able to reach the expected robust level. Finally, simulation results show attributes of the proposed method

The Compound Response Relationship between Hydro-Sedimentary Variations and Dominant Driving Factors: A Case Study of the Huangfuchuan basin

The Huangfuchuan basin is one of the major sources of coarse sediment in the Yellow River and has long been a focal point and challenge for the conservation of soil and water in the Yellow River Basin. In this study, we analyzed the phase differentiation characteristics of water–sediment variations during the flood season in the Huangfuchuan basin using a long-term dataset. We elucidated the complex response relationship between water–sediment variations and meteorological factors and human activities, which is of great significance for revealing the mechanisms of water–sediment variations in the region and establishing a scientific water–sediment regulation system in the basin. Statistical methods such as the Mann–Kendall trend test, Sen’s slope estimation, Pettitt nonparametric test, and principal component analysis were employed to identify and analyze the trends and dominant driving factors before and after the water–sediment variations and abrupt changes in parameters such as rainfall and temperature in the Huangfuchuan basin. Additionally, multiple regression analysis was used to determine the extent of the contribution of climate and human activities to water–sediment variations in the Huangfuchuan basin. The study revealed that the year 2000 was a turning point for water–sediment variations, with decreases of 11.3%, 76.7%, and 85.1% in flood season rainfall, flood season runoff, and flood season sediment transport, respectively. Despite significant changes in the underlying surface conditions of the Huangfuchuan basin, the relationship between flood season sediment transport and flood season runoff remained a power–law relationship. In the absence of obvious abrupt changes in temperature, rainfall, and other meteorological factors, the changes in the underlying surface caused by human activities are the main cause of the changes in runoff and sediment yield in the Huangfuchuan basin. The current level of vegetation restoration in the Huangfuchuan basin is still relatively low, making it difficult to exert stronger control on sediment yield during the flood season. Meanwhile, human activities, primarily based on engineering measures, play a more significant role in the control of soil and water loss in the basin