CCS-MPC for PMSM with Wide Speed Range based on Variable DC-Bus Voltage Control applied to the Flywheel Energy Storage System

In this paper, in order to verify the effect of variable DC-bus voltage control, CCS-MPC (Continuous-Control-Set MPC) control is used to research the control of PMSM (Permanent-Magnet Synchronous Machine) in a wide speed range. In order to maximize the speed of PMSM, the MTPA control, the field weakening control and the variable DC-bus voltage control are applied in the different speed range of PMSM. CCS-MPC can eliminate the control delay by controlling the predicted step size. When CCSMPC is applied to the PMSM control with a wide speed range, the advantages of strong adaptability of CCS-MPC can be exerted

A Novel Z-Type Self-Balancing Modular Multilevel Converter for Flywheel Driving Applications

With the development of flywheel technology, the speed and the voltage level of flywheel continue to increase. The Z-type self-balancing modular multilevel converter can have a wide frequency range at medium voltage and is suitable for flywheel driving application. However, the voltage and current characteristics of the flywheel and the variable frequency drive still affect capacitor voltage balance in each phase-leg. The new balance of capacitor voltage can be achieved by controlling the circulating current. This paper proposed a control algorithm of the novel Z-type self-balancing modular multilevel converter for flywheel driving applications. Simulation results are presented to validate the theoretical analysis

A Novel Variant of the FZD4 Gene in a Chinese Family Causes Autosomal Dominant Familial Exudative Vitreoretinopathy

Background/Aims: Familial exudative vitreoretinopathy (FEVR) is a complex hereditary eye disorder characterized by incomplete development of the retinal vasculature, thereby affecting retinal angiogenesis. Methods: In this study, a Chinese autosomal dominant FEVR pedigree was recruited. Ophthalmic examinations were performed, targeted next-generation sequencing was used to identify the causative gene, and Sanger sequencing was conducted to verify the candidate mutation. Co-segregation analysis was performed to evaluate pathogenicity. Semi-quantitative reverse transcription-PCR was applied to investigate the spatial and temporal expression patterns of the frizzled class receptor 4 (FZD4) gene in the mouse. Results: A novel heterozygous, deleterious variant of the FZD4 gene, c.A749G (p.Y250C), was identified in this FEVR pedigree, which co-segregated with the clinical phenotype. The amino acid tyrosine (Y) is highly conserved both orthologously and paralogously. The FZD4 gene was highly expressed in the retina, sclera of the eye, ovary, kidney, and liver; ubiquitously expressed in other tissues; and highly expressed in 6 different developmental stages/times of retinal tissue. Conclusion: Our study is the first to identify that the novel heterozygous variant c.A749G (p.Y250C) in the FZD4 gene may be the disease-causing mutation in this FEVR family, extending its mutation spectrum. These findings further our understanding of the molecular pathogenesis of FEVR and will facilitate the development of methods for the diagnosis, prevention, and genetic counseling of this disease

Genetic Identification and Molecular Modeling Characterization Reveal a Novel PROM1 Mutation in Stargardt4-like Macular Dystrophy

Stargardt disease-4 (STGD4) is an autosomal dominant complex, genetically heterogeneous macular degeneration/dystrophy (MD) disorder. In this paper, we used targeted next generation sequencing and multiple molecular dynamics analyses to identify and characterize a disease-causing genetic variant in four generations of a Chinese family with STGD4-like MD. We found a novel heterozygous missense mutation, c.734T\u3eC (p.L245P) in the PROM1 gene. Structurally, this mutation most likely impairs PROM1 protein stability, flexibility, and amino acid interaction network after changing the amino acid residue Leucine into Proline in the basic helix-loop-helix leucine zipper domain. Molecular dynamic simulation and principal component analysis provide compelling evidence that this PROM1 mutation contributes to disease causativeness or susceptibility variants in patients with STGD4-like MD. Thus, this finding defines new approaches in genetic characterization, accurate diagnosis, and prevention of STGD4-like MD

Analysis on a Stochastic Predator-Prey Model with Modified Leslie-Gower Response

This paper presents an investigation of asymptotic properties of a stochastic predator-prey model with modified Leslie-Gower response. We obtain the global existence of positive unique solution of the stochastic model. That is, the solution of the system is positive and not to explode to infinity in a finite time. And we show some asymptotic properties of the stochastic system. Moreover, the sufficient conditions for persistence in mean and extinction are obtained. Finally we work out some figures to illustrate our main results

Optimal Harvest of a Stochastic Predator-Prey Model

<p/> <p>We firstly show the permanence of hybrid prey-predator system. Then, when both white and color noises are taken into account, we examine the asymptotic properties of stochastic prey-predator model with Markovian switching. Finally, the optimal harvest policy of stochastic prey-predator model perturbed by white noise is considered.</p

CCS-MPC for PMSM with Wide Speed Range based on Variable DC-Bus Voltage Control applied to the Flywheel Energy Storage System

In this paper, in order to verify the effect of variable DC-bus voltage control, CCS-MPC (Continuous-Control-Set MPC) control is used to research the control of PMSM (Permanent-Magnet Synchronous Machine) in a wide speed range. In order to maximize the speed of PMSM, the MTPA control, the field weakening control and the variable DC-bus voltage control are applied in the different speed range of PMSM. CCS-MPC can eliminate the control delay by controlling the predicted step size. When CCSMPC is applied to the PMSM control with a wide speed range, the advantages of strong adaptability of CCS-MPC can be exerted

A Novel Z-Type Self-Balancing Modular Multilevel Converter for Flywheel Driving Applications

With the development of flywheel technology, the speed and the voltage level of flywheel continue to increase. The Z-type self-balancing modular multilevel converter can have a wide frequency range at medium voltage and is suitable for flywheel driving application. However, the voltage and current characteristics of the flywheel and the variable frequency drive still affect capacitor voltage balance in each phase-leg. The new balance of capacitor voltage can be achieved by controlling the circulating current. This paper proposed a control algorithm of the novel Z-type self-balancing modular multilevel converter for flywheel driving applications. Simulation results are presented to validate the theoretical analysis

A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

AbstractTo develop an economically feasible fermentation process, this study designed a novel bioprocess based on the co-culture of engineered Bacillus subtilis and Escherichia coli for the co-production of extracellular D-psicose and intracellular lipase. After optimizing the co-culture bioprocess, 11.70?g/L of D-psicose along with 16.03 U/mg of lipase was obtained; the glucose and fructose were completely utilized. Hence, the conversion rate of D-psicose reached 69.54%. Compared with mono-culture, lipase activity increased by 58.24%, and D-psicose production increased by 7.08%. In addition, the co-culture bioprocess was explored through metabolomics analysis, which included 168 carboxylic acids and derivatives, 70 organooxygen compounds, 34 diazines, 32 pyridines and derivatives, 30 benzene and substituted derivatives, and other compounds. It also could be found that the relative abundance of differential metabolites in the co-culture system was significantly higher than that in the mono-culture system. Pathway analysis revealed that, tryptophan metabolism and β-alanine metabolism had the highest correlation and played an important role in the co-culture system; among them, tryptophan metabolism regulates protein synthesis and β-alanine metabolism, which is related to the formation of metabolic by-products. These results confirm that the co-cultivation of B. subtilis and E. coli can provide a novel idea for D-psicose and lipase biorefinery, and are beneficial for the discovery of valuable secondary metabolites such as turanose and morusin

Process optimization for the production of high-concentration ethanol with Scenedesmus raciborskii biomass

Scenedesmus raciborskii WZKMT was subjected to fed-batch enzymatic hydrolysis and fermentation to facilitate the saccharification of high-solid-loading substrate for high-concentration ethanol. In this work, process factors affecting enzymatic hydrolysis, including enzyme loading, temperature, pH, and solid loading, were optimized. Results showed that 58.03 g L-1 glucose, 12.57 g L-1 xylose, and 1.45 g L-1 cellobiose were obtained after the enzymatic hydrolysis of 330 g L-1 substrates under the optimal conditions of 30 FPU g(-1) enzyme loading, 50 degrees C, and pH 5.5. Meanwhile, 89.60% yield and 30.43 g L-1 content of ethanol were obtained after the fermentation of 330 g L-1 hydrolysate. The maximum ethanol concentration of 79.38 g L-1 could be achieved through repeated fed-batch process, indicating that S. raciborskii WZKMT is a promising feedstock for ethanol production