Dynamic Mechanical Properties and Microstructure of Graphene Oxide Nanosheets Reinforced Cement Composites

This paper presents an experimental investigation on the effect of uniformly dispersed graphene oxide (GO) nanosheets on dynamic mechanical properties of cement based composites prepared with recycled fine aggregate (RFA). Three different amounts of GO, 0.05%, 0.10%, and 0.20% in mass of cement, were used in the experiments. The visual inspections of GO nanosheets were also carried out after ultrasonication by transmission electron microscope (TEM) atomic force microscope (AFM), and Raman to characterize the dispersion effect of graphite oxide. Dynamic mechanical analyzer test showed that the maximum increased amount of loss factor and storage modulus, energy absorption was 125%, 53%, and 200% when compared to the control sample, respectively. The flexural and compressive strengths of GO-mortar increased up to 22% to 41.3% and 16.2% to 16.4% with 0.20 wt % GO at 14 and 28 days, respectively. However the workability decreased by 7.5% to 18.8% with 0.05% and 0.2% GO addition. Microstructural analysis with environmental scanning electron microscopy (ESEM)/backscattered mode (BSEM) showed that the GO-cement composites had a much denser structure and better crystallized hydration products, meanwhile mercury intrusion porosimetry (MIP) testing and image analysis demonstrated that the incorporation of GO in the composites can help in refining capillary pore structure and reducing the air voids content

Numerical simulation of hydrodynamics and reaeration over a stepped spillway by the SPH method

Aerated flows are characterized by complex hydrodynamics and mass-transfer processes. As a Lagrangian method, smoothed particle hydrodynamics (SPH) has a significant advantage in tracking the air-water interface in turbulent flows. This paper presents the application of an SPH method to investigate hydrodynamics and reaeration over stepped spillways. In the SPH method, the entrainment of dissolved oxygen (DO) is studied using a multiphase mass transfer SPH method for reaeration. The numerical results are compared with the hydrodynamics data from Chanson and DO data from Cheng. The simulation results show that velocity distribution and the location of free-surface aeration inception agree with the experimental results. Compared with the experimental results, the distribution of DO concentration over the stepped spillway is consistent with the measurement results. The study shows that the two-phase DO mass transfer SPH model is reliable and reasonable for simulating the hydrodynamics characteristics and reaeration process

Dynamical Properties of Environmental High-Performance Composites with Calcined Clay

Concrete structures may be exposed to dynamic loadings within short periods such as earthquakes and vehicles load, resulting in substantial damage to human life and property because of the collapse of concrete structures. The research achievements on dynamic properties of high-performance composites with LC3 (HPC-LC3) are limited, although dynamic loadings are commonly encountered in infrastructure. The study aims to develop a new-green concrete product (HPC-LC3) with high dynamical properties and promote its mass use in a vibration service environment by investigating dynamical properties of HPC-LC3. Dynamical properties of structure can be promoted at material level by means of enhancing the inherent ability of cement-matrix materials to passively absorb the vibrational energy. Dynamic properties of traditional HPC may be improved by incorporated LC3 because of excellent mechanical properties. The fiber pull-out, thermogravimetric analysis (TGA), (nuclear magnetic resonance) NMR, and microstructure test were used to reveal the enhanced mechanism of dynamic properties in the HPC with LC3 (HPC-LC3), and the economic efficiency of HPC is also evaluated to promote its mass application. The results indicate that the dynamic properties of HPC-LC3 increased by 47% in damping capacity, 102% in storage modulus, 16% in energy dissipation, in comparison with the reference (HPC-OPC). Meanwhile, compared with the reference, the compressive and flexural strengths of LC3-50 increased by 14% and 27%. TGA and NMR results indicate that LC3-50 shows better hydration characteristics, longer alumina-silicate chain length (from 3.9 to 8) than HPC-OPC. The proposed composites reduced embodied energy, embedded CO2 emissions, and costs of HPC by up to 33%, 45%, and 7%, respectively. Thus, the green product (HPC-LC3) with 30%–40% calcined clay, high dynamic properties and more sustainable, will be recommended to mass use in a vibration service environment

Comparative study of the impact of dietary supplementation with different types of CpG oligodeoxynucleotides (CpG ODNs) on enhancing intestinal microbiota diversity, antioxidant capacity, and immune-related gene expression profiles in Pacific white shrimp (Litopenaeus vannamei)

The CpG oligodeoxynucleotides (CpG ODNs) reportedly possess the capacity to strengthen immunity in mammals. This experiment was conducted to evaluate the impact of dietary supplementation with 17 types of CpG ODNs on intestinal microbiota diversity, antioxidant capacity, and immune-related gene expression profiles of the shrimp Litopenaeus vannamei. Diets including 50 mg kg-1 CpG ODNs wrapped in egg whites were prepared and divided into 17 different groups, with 2 control groups (normal feed and feed with egg whites). These CpG ODNs supplemented diets and the control diets were fed to L. vannamei (5.15 ± 0.54 g) three times daily at 5%-8% shrimp body weight for three weeks. The results of consecutive detection of intestinal microbiota by 16S rDNA sequencing indicated that 11 of the 17 types of CpG ODNs significantly enhanced intestinal microbiota diversity, increased the populations of several probiotic bacteria, and activated possible mechanisms relevant to diseases. The immune-related genes expression and antioxidant capacity in hepatopancreas further demonstrated that the 11 types of CpG ODNs effectively improved the innate immunity of shrimp. Additionally, histology results showed that the CpG ODNs in the experiment did not damage the tissue structure of hepatopancreas. The results suggest that CpG ODNs could be used as a trace supplement to improve the intestinal health and immunity of shrimp

Stimulation of hair growth by Tianma Gouteng decoction: Identifying mechanisms based on chemical analysis, systems biology approach, and experimental evaluation

Hair serves important physiological functions, including temperature regulation and scalp protection. However, excessive shedding not only impacts these functions but can also significantly affect mental health and quality of life. Tianma Gouteng decoction (TGD) is a traditional Chinese medicine used for the treatment of various conditions, including hair loss. However, the associated mechanism underlying its anti-alopecia effect remains unknown. Therefore, this study aims to elucidate these mechanisms by employing systematic biology approaches, as well as in vitro and in vivo experimental validation. The chemical constituents of Tianma Gouteng decoction were identified using UHPLC-MS/MS, from which 39 potential bioactive components were screened, while an additional 131 putative Tianma Gouteng decoction beneficial components were extracted from the Traditional Chinese Medicine Database and Analysis Platform (TCMSP) database. We then applied a dual-dimensional network pharmacology approach to analyze the data, followed by validation studies combining molecular docking techniques with in vivo and in vitro experiments. From the 39 bioactive components, including quercetin, luteolin, fisetin, wogonin, oroxylin A, boldine, tetrahydroalstonine, and galangin A, 782 corresponding targets were identified. In particular, GSK3β and β-catenin exhibited strong binding activity with the bioactive compounds. Hence, construction of a bioactive component-target network revealed that the mechanism underlying the anti-alopecia mechanism of Tianma Gouteng decoction primarily involved the Wnt/β-catenin signaling pathway. Moreover, C57BL/6J mice exhibited measurable improvements in hair follicle regeneration following treatment with Tianma Gouteng decoction. Additionally, β-catenin and p-GSK3β levels were upregulated, while GSK3β was downregulated in Tianma Gouteng decoction-treated animals and dermal papilla cells compared to control group. These in vivo and in vitro outcomes validated the targets and pathways predicted in the network pharmacology analysis of Tianma Gouteng decoction. This study provides a systematic analysis approach to identify the underlying anti-alopecia mechanisms of Tianma Gouteng decoction, further providing theoretical support for clinical assessment of Tianma Gouteng decoction

SR-BI as a target of natural products and its significance in cancer

Scavenger receptor class B type I (SR-BI) protein is an integral membrane glycoprotein. SR-BI is emerging as a multifunctional protein, which regulates autophagy, efferocytosis, cell survival and inflammation. It is well known that SR-BI plays a critical role in lipoprotein metabolism by mediating cholesteryl esters selective uptake and the bi-directional flux of free cholesterol. Recently, SR-BI has also been identified as a potential marker for cancer diagnosis, prognosis, or even a treatment target. Natural products are a promising source for the discovery of new drug leads. Multiple natural products were identified to regulate SR-BI protein expression. There are still a number of challenges in modulating SR-BI expression in cancer and in using natural products for modulation of such protein expression. In this review, our purpose is to discuss the relationship between SR-BI protein and cancer, and the molecular mechanisms regulating SR-BI expression, as well as to provide an overview of natural products that regulate SR-BI expression

Urinary Aromatic Amino Acid Metabolites Associated With Postoperative Emergence Agitation in Paediatric Patients After General Anaesthesia: Urine Metabolomics Study

Background: Emergence agitation (EA) is very common in paediatric patients during recovery from general anaesthesia, but underlying mechanisms remain unknown. This prospective study was designed to profile preoperative urine metabolites and identify potential biomarkers that can predict the occurrence of EA.Methods: A total of 224 patients were screened for recruitment; of those, preoperative morning urine samples from 33 paediatric patients with EA and 33 non-EA gender- and age-matched patients after being given sevoflurane general anaesthesia were analysed by ultra-high-performance liquid chromatography (UHPLC) coupled with a Q Exactive Plus mass spectrometer. Univariate analysis and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA) were used to analyse these metabolites. The least absolute shrinkage and selection operator (LASSO) regression was used to identify predictive variables. The predictive model was evaluated through the receiver operating characteristic (ROC) analysis and then further assessed with 10-fold cross-validation.Results: Seventy-seven patients completed the study, of which 33 (42.9%) patients developed EA. EA and non-EA patients had many differences in preoperative urine metabolic profiling. Sixteen metabolites including nine aromatic amino acid metabolites, acylcarnitines, pyridoxamine, porphobilinogen, 7-methylxanthine, and 5′-methylthioadenosine were found associated with an increased risk of EA, and they all exhibited higher levels in the EA group than in the non-EA group. The main metabolic pathways involved in these metabolic changes included phenylalanine, tyrosine and tryptophan metabolisms. Among these potential biomarkers, L-tyrosine had the best predictive value with an odds ratio (OR) (95% CI) of 5.27 (2.20–12.63) and the AUC value of 0.81 (0.70–0.91) and was robust with internal 10-fold cross-validation.Conclusion: Urinary aromatic amino acid metabolites are closely associated with EA in paediatric patients, and further validation with larger cohorts and mechanistic studies is needed.Clinical Trial Registration:clinicaltrials.gov, identifier NCT0480799

Frequency-Dependent Relationship Between Resting-State fMRI and Glucose Metabolism in the Elderly

Both glucose metabolism and resting-state fMRI (RS-fMRI) signal reflect hemodynamic features. The objective of this study was to investigate their relationship in the resting-state in healthy elderly participants (n = 18). For RS-fMRI signal, regional homogeneity (ReHo), amplitude of low frequency fluctuations (ALFF), fractional ALFF (fALFF), and degree of centrality (DC) maps were generated in multiple frequency bands. Glucose uptake was acquired with 18F-fluorodeoxyglucose positron emission tomography (FDG-PET). Linear correlation of each pair of the FDG-PET and RS-fMRI metrics was explored both in across-voxel way and in across-subject way. We found a significant across-voxel correlation between the FDG-PET and BOLD-fMRI metrics. However, only a small portion of voxels showed significant across-subject correlation between FDG-PET and BOLD-fMRI metrics. All these results were similar across all frequency bands of RS-fMRI data. The current findings indicate that FDG-PET and RS-fMRI metrics share similar spatial pattern (significant across-voxel correlation) but have different underlying physiological importance (non-significant across-subject correlation). Specifically, FDG-PET measures the mean glucose metabolism over tens of minutes, while RS-fMRI measures the dynamic characteristics. The combination of FDG-PET and RS-fMRI provides complementary information to reveal the underlying mechanisms of the brain activity and may enable more comprehensive interpretation of clinical PET-fMRI studies. Future studies would attempt to reduce the artifacts of RS-fMRI and to analyze the dynamic feature of PET signal

Electromagnetic characteristics of square cold crucible designed for silicon preparation

In the present work, the strength and distribution of electromagnetic field in the square cold crucible that designed for casting multicrystalline silicon were measured and analyzed by using a small coil method. The results show that in the perpendicular direction the maximum of magnetic flux density (B) appears at the position slightly above the middle of the coil, and then B attenuates toward both sides, and decreases more to the bottom of the crucible. In the horizontal direction, from the edge (corner) to the center, B firstly decreases gradually, and then slightly increases in the center. While along the inner sides of the crucible, the distribution is relatively uniform, especially in the effective acting range. B increases with the increasing of the input power. Moving the coil to the top of the crucible, B increases and the effective acting range of the electromagnetic field becomes bigger. For the coils with different turns, the five turns coil can induce the highest magnetic flux density

China’s First Demonstration of Cobalt-rich Manganese Crust Thickness Measurement in the Western Pacific with a Parametric Acoustic Probe

Cobalt-rich manganese crusts (CRCs) are important as a potential mineral source that could occur throughout the Pacific on seamounts, ridges, and plateaus. We built a prototype parametric acoustic probe to complete the task of in-situ thickness measurements to estimate the volumetric distribution of deep-sea mineral. The prototype is designed with dual-channels for receiving the primary and secondary signal, which lays a foundation for improving the thickness extraction algorithm. Considering that the signal quality is degraded by the system interference and ambient noise, some improvements to the algorithm are proposed by including the wavelet-based envelope extraction method and the adaptive estimation strategy based on the dual-channel information. Additionally, wavelet regression is applied to reduce the measuring noise assuming that the CRCs have local thickness invariability. The algorithm is suitable for the CRCs with the structure of the multilayers at the top surface and one single layer at the bottom surface. A laboratory experiment is performed to validate the effectiveness of the algorithm. The experiments carried out on the China Ocean 51th voyage in the Western Pacific Ocean on Aug 30, 2018, are described and the data obtained by using the sit-on-bottom stationary measurement are processed to validate the design of the prototype