The Optimum operating conditions for extraction of chlorophyll from Alternanthera sessilis(Linn.), cultivated in Sri Lanka

Chlorophyll is widely extracted for industrial applications and is a key topic of scientific and commercial interest. Alternanthera sessilis, which is known as mukunuwenna in Sri Lanka, is one of best selections for the extraction of chlorophyll due to the good extraction efficiency, availability and low cost. In this work, the optimum operating conditions for the extraction of chlorophyll from A. sessilis using solvent extraction were studied using buffered 80% aqueous acetone. The results revealed that the best solvent volume to A. sessilis weight ratio, which gives the highest yield of chlorophyll, was 5 ml/g. The effect of temperature and the extraction time on the extraction was also studied. The optimum temperature of extraction is 50°C and the optimum time of extraction is 45 minutes. The mass transfer of chlorophyll from A. sessilis at 20°C and 30°C was modeled mathematically, using general mass transfer equations. The experimental results showed that the degradation of chlorophyll beyond the temperature of 30°C is significant. The saturation solubility of chlorophyll a was 54.06 and 107.6μg/ml and that for chlorophyll b was 23.13 and 29.68 μg/ml at the temperatures of 20 and 30°C respectively. Furthermore, the optimum pre-processing method and the storage conditions were studied as post harvest operations for A. sessilis. The optimum method of pre processing was identified as mechanical grinding. For one day storage, ambient air conditions (indoor) were the optimum conditions; and for a longer storage time it was the refrigerator conditions (15oC). Using the extract, commercial chlorophyll, Sodium copper chlorophyllin has produced with a 33.3% conversion of chlorophyll and its derivatives, using a modified process. The kinetics of degradation of chlorophyll of the produced sodium copper chlorophyllin follows a first order relationship between the concentration and the time. The relationship between the rate constant and the temperature follows the Arrhenius behavior. The activation energy for chlorophyll a and b are 3.014 and 2.78 kcal/mol respectively. The rate constants for chlorophyll a and b at different temperatures are also obtained

Study the effect of temperature on extraction of chlorophyll from alternanthera sessilis cultivated in Sri Lanka

The effect of temperature on extraction of chlorophyll from Alternanthera sessilis cultivated in Sri Lanka was studied using buffered 80% aqueous acetone as the solvent. The yield increased with the increase of temperature up to 40°C. At temperatures above 40°C, the degradation of chlorophyll during the extraction was significant

Study on pre processing and storage conditions for extraction of chlorophyll from alternantherasessilis (L.) and kinetics of degradation of chlorophyll,

Extraction of chlorophyll from various plants is a key topic of scientific and commercial interest. Alternantherasessilis was used for theextraction of chlorophyll as a locally available plant in Sri Lanka. The effect of methods of pre processing and storage onditions of A. sessilis on the chlorophyll content was studied. Mechanical grinding was found to be asuperior pre-processing method compared to blanching and drying. Optimum storage conditions were ambient temperature for one day storage and refrigeration at 15°C for more than one day storage. The kinetic studies on chlorophyll degradation revealed that bothchlorophyll a and b degradation followed the first-order reaction model. The temperature dependence of the rate constant was adequately modeled by the Arrhenius equation. The activation energy values were 3.0143 kcal/mol and 2.78 kcal/mol for chlorophyll a and chlorophyll b , respective

Extraction and degradation of chlorophyll a and b from AlternantheraSessilis

The use of Alternanthera sessilis, which is commonly known as Mukunuwenna in Sri Lanka as a source of chlorophyll was examined. The extraction of chlorophyll was carried out using buffered 80 % (v/v) aqueous acetone. The optimum operating conditions such as solvent to A. sessilis ratio, extraction temperature and extraction time were found to be 5 mL/g, 50 °C and 45 minutes, respectively. The yield of chlorophyll a and chlorophyll b under these optimum operating conditions were 659 and 261 μg/g of A. sessilis, respectively. Mechanical grinding of A. sessilis gave a higher yield as compared to blanching and drying. Refrigeration at 15 °C was found to be ideal for storing of fresh A. sessilis up to 3 days without a considerable loss of chlorophyll content. Chlorophyll extraction could be modelled successfully using basic mass transfer equations up to 30 °C. It failed above this temperature due to the degradation effect. Kinetic study on the degradation of chlorophyll extracted from A. sessilis confirmed first order reaction model and the effect of temperature on the rate constant was also adequately modelled by the Arrhenius equation

Study on pre processing and storage conditions for extraction of chlorophyll from altemantherasessilis (L.) and knetics of degradation of chlorophyll

Extraction of chlorophyll from various plants is a key topic of scientific and commercial interest Altemantherasessilis was used for theextraction of chlorophyll as a locally available plant in Sri Lanka. The effect of methods of pre processing and storage conditions of A. sessilis on the chlorophyll content was studied. Mechanical grinding was found to be asuperior pre-processing method compared to blanching and drying. Optimum storage conditions were ambient temperature for one day storage and refrigeration at 15°C for more than one day storage. The kinetic studies on chlorophyll degradation revealed that bothchlorophyll a-and b degradation followed the first-order reaction model. The temperature dependence of the rate constant was adequately modeled by the Arrhenius equation. The activation energy values were 3.0143 kcal/mol and 2.78 kcal/mol for chlorophyll a and chlorophyll b, respectively

Mathematical modeling of extraction of chlorophyll from alternanthera sessilis cultivated in Sri Lanka

Mass transfer modeling was used to study the extraction efficiency and the effect of temperature on solvent extraction of chlorophyll from. Alternanthera sessilis, which is a leafy vegetable cultivated in tropics and sub tropics. Aqueous I acetone with 80% (v/v) was used as the solvent and the chlorophyll concentration was analyzed spectrophotometrically. The experimental results were in good agreement with the model data for temperatures | below 30 C. The degradation of chlorophyll beyond 30 C was found to be significant

Study on extraction of chlorophyll from Altemanthera Sessilis (Linn.) : operating conditions and mass transfer

Chlorophyll is a widely used ingredient for various applications including medicines, food supplements, cosmetics and toiletries. It is extracted traditionally from alfalfa, spinach and algae. Altemanthera sessilis, commonly known as mukunuwenna available in Sri Lanka is proposed for the extraction of chlorophyll The extraction was carried out using buffered 80% aqueous acetone. The optimum operating conditions; solvent to A. sessilis ratio, extraction temperature and extraction time found to be 5 ml/g, 50°C and 45 minutes, respectively. The yield of chlorophyll a and chlorophyll b under the optimum operating conditions are 659 and 261 ug/g of A. sessilis respectively and comparison of results obtained on yield with the reported data is presented. Furthermore, the transfer from the soiid to the solvent for various operating temperatures was modelled and Abstract: were mass validated with the experimental results