23,093 research outputs found

    LTR retrotransposon landscape in Medicago truncatula: more rapid removal than in rice

    Get PDF
    <p>Abstract</p> <p>Background</p> <p>Long terminal repeat retrotransposons (LTR elements) are ubiquitous Eukaryotic TEs that transpose through RNA intermediates. Accounting for significant proportion of many plant genomes, LTR elements have been well established as one of the major forces underlying the evolution of plant genome size, structure and function. The accessibility of more than 40% of genomic sequences of the model legume <it>Medicago truncatula </it>(<it>Mt</it>) has made the comprehensive study of its LTR elements possible.</p> <p>Results</p> <p>We use a newly developed tool LTR_FINDER to identify LTR retrotransposons in the <it>Mt </it>genome and detect 526 full-length elements as well as a great number of copies related to them. These elements constitute about 9.6% of currently available genomic sequences. They are classified into 85 families of which 64 are reported for the first time. The majority of the LTR retrotransposons belong to either Copia or Gypsy superfamily and the others are categorized as TRIMs or LARDs by their length. We find that the copy-number of Copia-like families is 3 times more than that of Gypsy-like ones but the latter contribute more to the genome. The analysis of PBS and protein-coding domain structure of the LTR families reveals that they tend to use only 4–5 types of tRNAs and many families have quite conservative ORFs besides known TE domains. For several important families, we describe in detail their abundance, conservation, insertion time and structure. We investigate the amplification-deletion pattern of the elements and find that the detectable full-length elements are relatively young and most of them were inserted within the last 0.52 MY. We also estimate that more than ten million bp of the <it>Mt </it>genomic sequences have been removed by the deletion of LTR elements and the removal of the full-length structures in <it>Mt </it>has been more rapid than in rice.</p> <p>Conclusion</p> <p>This report is the first comprehensive description and analysis of LTR retrotransposons in the <it>Mt </it>genome. Many important novel LTR families were discovered and their evolution is elucidated. Our results may outline the LTR retrotransposon landscape of the model legume.</p

    Multifractal analysis of weighted networks by a modified sandbox algorithm

    Get PDF
    Complex networks have attracted growing attention in many fields. As a generalization of fractal analysis, multifractal analysis (MFA) is a useful way to systematically describe the spatial heterogeneity of both theoretical and experimental fractal patterns. Some algorithms for MFA of unweighted complex networks have been proposed in the past a few years, including the sandbox (SB) algorithm recently employed by our group. In this paper, a modified SB algorithm (we call it SBw algorithm) is proposed for MFA of weighted networks.First, we use the SBw algorithm to study the multifractal property of two families of weighted fractal networks (WFNs): "Sierpinski" WFNs and "Cantor dust" WFNs. We also discuss how the fractal dimension and generalized fractal dimensions change with the edge-weights of the WFN. From the comparison between the theoretical and numerical fractal dimensions of these networks, we can find that the proposed SBw algorithm is efficient and feasible for MFA of weighted networks. Then, we apply the SBw algorithm to study multifractal properties of some real weighted networks ---collaboration networks. It is found that the multifractality exists in these weighted networks, and is affected by their edge-weights.Comment: 15 pages, 6 figures. Accepted for publication by Scientific Report

    2-Phenyl-1H-imidazol-3-ium hydrogen oxalate

    Get PDF
    In the title mol­ecular salt, C9H9N2 +·C2HO4 −, the dihedral angle between the aromatic rings of the cation is 17.5 (3)° and the dihedral angle between the –CO2H and –CO2 groups of the anion is 38.6 (2)°. In the crystal, the components inter­act by way of O—H⋯O and N—H⋯O hydrogen bonds
    corecore