Non-slipping adhesive contact of rigid cylinder on an elastic power-law graded half-space

In this paper, adhesive contact of a rigid cylinder on an elastic power-law graded half-space is studied analytically with the theory of weakly singular integral equation and orthogonal polynomial method. Emphasis is placed on the coupling effect between tangential and normal directions which was often neglected in previous works. Our analysis shows that the coupling effect tends to minimize the contact area in the compressive regime. The effect of bending moment on the adhesion behavior is also examined. Like a pull-off force, there also exist a critical bending moment at which the cylinder can be bended apart from the substrate. However, unlike pull-off force, the critical bending moment is insensitive to the gradient exponent of the graded material

Screening for the optimal siRNA targeting a novel gene (HA117) and construction and evaluation of a derivative recombinant adenovirus

We found a novel gene named as HA117 in our previous research. At this study, we screened for an optimal siRNA targeting the novel gene HA117 using the pSOS-HUS method, verified the results of pSOS-HUS siRNA screening for optimal affinity for the target gene, and constructed and evaluated a recombinant adenovirus carrying the DNA template for transcription of the optimal HA117 siRNA. The pSOS-HUS vector method was successfully utilized as a rapid and effective screen for an optimal siRNA for a target gene. Among five pairs of DNA templates, siRNA transcribed from HAi5 gave the strongest interference with the novel gene HA117; a HAi5-carrying recombinant adenovirus (Ad-HAi5) was successfully constructed and evaluated, laying a foundation for the further study of HA117 gene function with RNAi technology