Empleo del BIOBRAS-6 en la micropropagación del cultivar de plátano FHIA –21 (AAAB)

With the objective of evaluating the effectiveness of BIOBRAS-6 in the micropropagation of the plantain cultivar FHIA-21, the present work was carried out during the period of January 2000 to September 2001, in the laboratory of Mass Propagation of the Institute of Plant Biotechnology. Different concentrations (0.05, 0.01 mg.l-1) and combinations of this product were studied with growth regulators for multiplication, in vitro rooting and acclimatization. Results obtained in the experiments of the different phases had a marked effect of auxin reinforcement of BIOBRAS-6. In the multiplication phase BIOBRAS-6 (0.05 mg.l-1) combined with 6-Benzilaminopurine(BAP) (4 mg.l-1) presented growth indicators superior to the plants in vitro as well as in the rooting phase as supplement of the culture medium with Indolacetic acid(IAA) (0.65 mg.l-1). A superior development was also observed in the in vitro plants in the acclimatization phase after submerging the roots in a solution of water with Naftalenacetic acid (NAA) (10 mg.l-1) and BIOBRAS-6 (0.05 mg.l-1). In general the use of BIOBRAS-6 in the micropropagation of the hybrid FHIA-21, increased the principal indicators for the different stages.reinforcementKey words: brasinosteroids, quality, in vitro plants, auxinCon la finalidad de evaluar la efectividad del BIOBRAS-6 en la micropropagación del cultivar de plátano FHIA-21(AAAB) se llevó a cabo el presente trabajo durante el período de enero del 2000 a septiembre del 2001, en el laboratorio de Propagación Masiva, del Instituto de Biotecnología de las Plantas (IBP). Se estudiaron diferentes concentraciones (0.05 y 0.01 mg.l-1) y combinaciones de este producto con reguladores del crecimiento para la multiplicación y enraizamiento in vitro y la aclimatización. Los resultados mostraron un marcado efecto de reforzamiento auxínico del BIOBRAS-6 para las diferentes etapas. En la fase de multiplicación el BIOBRAS-6 (0.05 mg.l-1) combinado con 6-Benzilaminopurina (6BAP) (4 mg.l-1) presentó los indicadores superiores del crecimiento de las plantas in vitro, al igual que en la fase de enraizamiento como suplemento del medio de cultivo con Acido indolacético (AIA) 0.65 mg.l-1. También se observó un desarrollo superior de las plantas in vitro en la fase de aclimatización después de la inmersión de las raíces en la solución de agua común con BIOBRAS-6 (0.05 mg.l-1) y Acido naftalenacético (ANA) (10 mg.l-1). De forma general el empleo del BIOBRAS-6 en la micropropagación del cultivar híbrido FHIA-21 incrementó los principales indicadores del crecimiento para las diferentes etapas.Palabras clave: brasinoesteroide, calidad, plantas in vitro, reforzamiento auxínic

Aclimatización de plantas in vitro de Solanum tuberosum (L.) variedad Desiree

In the phase of acclimatization of the Plants Biotechnology Institute of the Central University of Las Villas the present work was done with the objective of development the methodology of acclimatization of potato plants In vitro in function of the seed production. The results demostrated that the plants in vitro coming from the rooting phase bigger than 4.0cm had a better behavior, that together to the handling of other factors like the employment of an appropriate substrat based on the compost an formulation casting alone or cocktails with 15 zeolita%, the nitrogen fertilization (urea 2.5 g.l) and the regulation of the solar illumination to 25 or 50% in the first week and 50 or 75% in second, allowed to increase the physiologic quality of the vitroplants to be planted in the field.Keywords: in vitro plants, in vivo conditions, management, sustratesEn la fase de aclimatización del Instituto de Biotecnología de las Plantas de la UCLV se realizó el presente trabajo con el objetivo de desarrollar la metodología de aclimatización de plantas in vitro de papa en función de la producción de semilla. Los resultados demostraron que las plantas in vitro procedentes de la fase de enraizamiento mayores de 4.0cm tuvieron un mejor comportamiento, además, con el sustrato basado en las formulaciones de compost y casting solos o combinados con 15% de zeolita, la fertilización nitrogenada (urea 2.5 gl-¹) e iluminación solar de 25 ó 50% en la primera semana y 50 ó 75% en la segunda se incrementó la calidad fisiológica de las plantas in vitro para ser plantadas en el campo.Palabras clave: condiciones in vivo, manejo, plantas in vitro, sustrat

Efecto de una formulación de propóleo en los medios de cultivo para la micropropagación de la papa var Desirée

In the efforts to improve the quality of the plants in the in vitro culture, different alternatives have been tested and put into practice going from the increasing of the asepsis measures, chemical treatment of the plants donors, to the subculture of plants in cultures media with antimicrobial products of synthetic or natural origin. At the Institute of Plants Biotechnology, at the Central University of Las Villas, the influence of a formulation of propolis was evaluated on the growth and multiplication of in vitro plants of potato (Solanum tuberosum L.) from Desirée variety. The phytotoxic effect of this product in culture media was determined in the in vitro multiplication phase, and dilutions of the propolis were made to obtain concentrations from 1 to 200 ppm in ranges of 10 units. After the phytotoxic evaluation the adequate propolis working dose was applied during three subcultures in the in vitro multiplication phase in a comparative way with two treatments; culture medium elaborated with ebullition methods for five minutes. A subculture was also carried out in the in vitro rooting phase and the behavior of these plants in the acclimatization phase was evaluated. Alterations in levels superior to 80 ppm were observed in the morphologic variables evaluated. The treatment with the formulation of propoli presented significantly superior results than control.Key words: aclimatization, multiplication, rootingCon la intención de acelerar el crecimiento de las plantas en el cultivo in vitro, se han estudiado diferentes alternativas entre las que se encuentran el manejo de las condiciones de cultivo y la aplicación de sustancias estimuladoras del crecimiento a los medios de cultivo. El propóleo es un producto de origen natural, a partir de la miel de las abejas; que posee compuestos con una variada actividad biológica, pero aún no se conoce bien la influencia de su aplicación en el crecimiento y desarrollo de las plantas. El presente trabajo se realizó con el objetivo de evaluar el efecto de una formulación del propóleo en los medios de cultivo para la micropropagación de la papa (Solanum tuberosum L.) variedad Desirée. Se determinó el efecto fitotóxico de este producto en la fase de multiplicación in vitro y además, se evaluó su acción sobre las plantas in vitro en la fase enraizamiento y en la fase de aclimatización. Se observaron alteraciones en las variables evaluadas a las plantas in vitro en fase de multiplicación cuando se añadió al medio de cultivo el propóleo desde 80ppm hasta 200ppm lo que indicó fitotoxicidad de la formulación. Sin embargo, a la concentración de 70ppm las plantas in vitro alcanzaron resultados superiores al control en las variables evaluadas, durante todas las fases de la micropropagación.Palabras clave: aclimatización, enraizamiento, multiplicació

Use of Biobras-6 in the micropropagation of the plantain cultivar FHIA-21 (AAAB)

With the objective of evaluating the effectiveness of BIOBRAS-6 in the micropropagation of the plantain cultivar FHIA-21, the present work was carried out during the period of January 2000 to September 2001, in the laboratory of Mass Propagation of the Institute of Plant Biotechnology. Different concentrations (0.05, 0.01 mg.l-1) and combinations of this product were studied with growth regulators for multiplication, in vitro rooting and acclimatization. Results obtained in the experiments of the different phases had a marked effect of auxin reinforcement of BIOBRAS-6. In the multiplication phase BIOBRAS-6 (0.05 mg.l-1) combined with 6-Benzilaminopurine(BAP) (4 mg.l-1) presented growth indicators superior to the plants in vitro as well as in the rooting phase as supplement of the culture medium with Indolacetic acid(IAA) (0.65 mg.l-1). A superior development was also observed in the in vitro plants in the acclimatization phase after submerging the roots in a solution of water with Naftalenacetic acid (NAA) (10 mg.l-1) and BIOBRAS-6 (0.05 mg.l-1). In general the use of BIOBRAS-6 in the micropropagation of the hybrid FHIA-21, increased the principal indicators for the different stages. reinforcement Key words: brasinosteroids, quality, in vitro plants, auxi

<i>In vitro</i> plants aclimatization of <i>Solanum tuberosum</i> (L.) var. Desiree

In the phase of acclimatization of the Plants Biotechnology Institute of the Central University of Las Villas the present work was done with the objective of development the methodology of acclimatization of potato plants In vitro in function of the seed production. The results demostrated that the plants in vitro coming from the rooting phase bigger than 4.0cm had a better behavior, that together to the handling of other factors like the employment of an appropriate substrat based on the compost an formulation casting alone or cocktails with 15 zeolita%, the nitrogen fertilization (urea 2.5 g.l) and the regulation of the solar illumination to 25 or 50% in the first week and 50 or 75% in second, allowed to increase the physiologic quality of the vitroplants to be planted in the field. Keywords: in vitro plants, in vivo conditions, management, sustrate

Effect of propolis formulation on culture medium for potato micropropagation

In the efforts to improve the quality of the plants in the in vitro culture, different alternatives have been tested and put into practice going from the increasing of the asepsis measures, chemical treatment of the plants donors, to the subculture of plants in cultures media with antimicrobial products of synthetic or natural origin. At the Institute of Plants Biotechnology, at the Central University of Las Villas, the influence of a formulation of propolis was evaluated on the growth and multiplication of in vitro plants of potato (Solanum tuberosum L.) from Desirée variety. The phytotoxic effect of this product in culture media was determined in the in vitro multiplication phase, and dilutions of the propolis were made to obtain concentrations from 1 to 200 ppm in ranges of 10 units. After the phytotoxic evaluation the adequate propolis working dose was applied during three subcultures in the in vitro multiplication phase in a comparative way with two treatments; culture medium elaborated with ebullition methods for five minutes. A subculture was also carried out in the in vitro rooting phase and the behavior of these plants in the acclimatization phase was evaluated. Alterations in levels superior to 80 ppm were observed in the morphologic variables evaluated. The treatment with the formulation of propoli presented significantly superior results than control. Key words: aclimatization, multiplication, rootin

Measurement of the W±ZW^{\pm}Z boson pair-production cross section in pppp collisions at s=13\sqrt{s}=13 TeV with the ATLAS Detector

The production of $W^{\pm}Z$ events in proton--proton collisions at a centre-of-mass energy of 13 TeV is measured with the ATLAS detector at the LHC. The collected data correspond to an integrated luminosity of 3.2 fb$^{-1}$. The $W^{\pm}Z$ candidates are reconstructed using leptonic decays of the gauge bosons into electrons or muons. The measured inclusive cross section in the detector fiducial region for leptonic decay modes is $\sigma_{W^\pm Z \rightarrow \ell^{'} \nu \ell \ell}^{\textrm{fid.}} = 63.2 \pm 3.2$ (stat.) $\pm 2.6$ (sys.) $\pm 1.5$ (lumi.) fb. In comparison, the next-to-leading-order Standard Model prediction is $53.4^{+3.6}_{-2.8}$ fb. The extrapolation of the measurement from the fiducial to the total phase space yields $\sigma_{W^{\pm}Z}^{\textrm{tot.}} = 50.6 \pm 2.6$ (stat.) $\pm 2.0$ (sys.) $\pm 0.9$ (th.) $\pm 1.2$ (lumi.) pb, in agreement with a recent next-to-next-to-leading-order calculation of $48.2^{+1.1}_{-1.0}$ pb. The cross section as a function of jet multiplicity is also measured, together with the charge-dependent $W^+Z$ and $W^-Z$ cross sections and their ratio

Search for Scalar Diphoton Resonances in the Mass Range 65−60065-600 GeV with the ATLAS Detector in pppp Collision Data at s\sqrt{s} = 8 TeVTeV

A search for scalar particles decaying via narrow resonances into two photons in the mass range 65–600 GeV is performed using $20.3\text{}\text{}{\mathrm{fb}}^{-1}$ of $\sqrt{s}=8\text{}\text{}\mathrm{TeV}$ $pp$ collision data collected with the ATLAS detector at the Large Hadron Collider. The recently discovered Higgs boson is treated as a background. No significant evidence for an additional signal is observed. The results are presented as limits at the 95% confidence level on the production cross section of a scalar boson times branching ratio into two photons, in a fiducial volume where the reconstruction efficiency is approximately independent of the event topology. The upper limits set extend over a considerably wider mass range than previous searches

Search for Higgs and ZZ Boson Decays to J/ψγJ/\psi\gamma and Υ(nS)γ\Upsilon(nS)\gamma with the ATLAS Detector

A search for the decays of the Higgs and $Z$ bosons to $J/\psi\gamma$ and $\Upsilon(nS)\gamma$ ($n=1,2,3$) is performed with $pp$ collision data samples corresponding to integrated luminosities of up to $20.3\mathrm{fb}^{-1}$ collected at $\sqrt{s}=8\mathrm{TeV}$ with the ATLAS detector at the CERN Large Hadron Collider. No significant excess of events is observed above expected backgrounds and 95% CL upper limits are placed on the branching fractions. In the $J/\psi\gamma$ final state the limits are $1.5\times10^{-3}$ and $2.6\times10^{-6}$ for the Higgs and $Z$ bosons, respectively, while in the $\Upsilon(1S,2S,3S)\,\gamma$ final states the limits are $(1.3,1.9,1.3)\times10^{-3}$ and $(3.4,6.5,5.4)\times10^{-6}$, respectively