TRIM29 acts as a potential senescence suppressor with epigenetic activation in nasopharyngeal carcinoma.

Epigenetic alterations marked by DNA methylation are frequent events during the early development of nasopharyngeal carcinoma (NPC). We identified that TRIM29 is hypomethylated and overexpressed in NPC cell lines and tissues. TRIM29 silencing not only limited the growth of NPC cells in vitro and in vivo, but also induced cellular senescence, along with reactive oxygen species (ROS) accumulation. Mechanistically, we found that TRIM29 interacted with voltage-dependent anion-selective channel 1 (VDAC1) to activate mitophagy clearing up damaged mitochondria, which are the major source of ROS. In patients with NPC, high levels of TRIM29 expression are associated with an advanced clinical stage. Moreover, we detected hypomethylation of TRIM29 in patient nasopharyngeal swab DNA. Our findings indicate that TRIM29 depends on VDAC1 to induce mitophagy and prevents cellular senescence by decreasing ROS. Detection of aberrantly methylated TRIM29 in the nasopharyngeal swab DNA could be a promising strategy for the early detection of NPC

Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

AbstractWe developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings

Optical thermal filters for eXTP: Manufacturing and characterization

In order to ensure the effective detection of X-ray astronomical detectors by blocking ultraviolet, visible and infrared light, adding optical thermal filter in front of the load is an effective method. According to the scientific requirements of eXTP, optical thermal filters with aluminized polyimide (PI) film structure had been designed and tested in this paper, the results of mechanical tests including burst pressure, vibration and acoustic tests, also the transparent properties of optics in UV, Vis and IR lights are presented. The mechanical test results show that the filters for LAD and SFA can't pass the acoustic tests, causing the thickness of PI should be increased or a nickel mesh structure should be added. Furthermore, the transmission test results indicate that a single-sided Al deposited structure is more suitable than a double-sided one

The large area detector onboard the eXTP mission

The Large Area Detector (LAD) is the high-throughput, spectral-timing instrument onboard the eXTP mission, a flagship mission of the Chinese Academy of Sciences and the China National Space Administration, with a large European participation coordinated by Italy and Spain. The eXTP mission is currently performing its phase B study, with a target launch at the end-2027. The eXTP scientific payload includes four instruments (SFA, PFA, LAD and WFM) offering unprecedented simultaneous wide-band X-ray timing and polarimetry sensitivity. The LAD instrument is based on the design originally proposed for the LOFT mission. It envisages a deployed 3.2 m2 effective area in the 2-30 keV energy range, achieved through the technology of the large-area Silicon Drift Detectors - offering a spectral resolution of up to 200 eV FWHM at 6 keV - and of capillary plate collimators - limiting the field of view to about 1 degree. In this paper we will provide an overview of the LAD instrument design, its current status of development and anticipated performance

Exploring beginning teachers’ agency : a case study of three Chinese secondary school teachers

The purpose of this research was to explore beginning teachers’ agency at a time of curriculum reform in China and in the context of an early-career mentoring program. Guided by a subject-centered sociocultural approach (Eteläpelto, Vähäsantanen, Hökkä, & Paloniemi, 2013), this qualitative study utilized a case study design. Data sources included semi-structured interviews with three secondary school chemistry teachers, signed mentorship agreement documents, and reflective mentoring/teaching journals. The findings revealed different forms of novice teachers’ agency in educational change, i.e., proactive agency and passive agency, manifested as acceptance and resistance to curriculum change. Beginning teachers’ pedagogical decisions and actions were the result of their critical analysis of multiple factors. On the one hand, school culture and structure must be carefully considered if agency is to be realized in a professional setting. Specifically, sociocultural factors included the status of the exam-oriented education system, mentor power, and work cultures. On the other hand, although novice teachers were embedded in similar sociocultural contexts, their identity impacted the different ways in which they exercised their agency. This study strongly supported the interrelationship between teacher agency and teacher identity. Teacher identity factors involved work history and experience, professional knowledge and competence, professional commitment, motivations and interests, and professional goals and ideals. This study provides rich perspectives on beginning teachers’ agency and its manifestations in the context of curriculum reform and in relation to early-career mentoring; findings serve as a strong scaffold for future research on how mentoring better promotes novice teachers’ agency and ultimately, professional development. The findings also offer lessons for those interested in investigating novice teachers’ agency in other educational settings undergoing similar changes.Education, Faculty ofCurriculum and Pedagogy (EDCP), Department ofGraduat