Use of Self-Assembly Nanofibre Biomaterials for Neural Repair After Injury

published_or_final_versio

Soluble NgR Fusion Protein Modulates the Proliferation of Neural Progenitor Cells via the Notch Pathway

NogoA, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein are CNS myelin molecules that bind to the neuronal Nogo-66 receptor (NgR) and inhibit axon growth. The NgR antagonist, soluble NgR1-Fc protein (sNgR-Fc), facilitates axon regeneration by neutralizing the inhibitory effects of myelin proteins in experimental models of CNS injury. Here we aim to investigate the effect of sNgR-Fc on the proliferation of neural progenitor cells (NPCs). The hippocampus cells of embryonic rats were isolated and cultured in vitro. The expression of nestin, βIII-Tubulin, GFAP and Nogo-A on these cells was observed using immunocytochemistry. In order to investigate the effect on proliferation of NPCs, sNgR-Fc, MAG-Fc chimera and Notch1 blocker were added respectively. The total cell number for the proliferated NPCs was counted. BrdU was applied and the rate of proliferating cells was examined. The level of Notch1 was analyzed using Western blotting. We identified that NogoA is expressed in NPCs. sNgR-Fc significantly enhanced the proliferation of NPCs in vitro as indicated by BrdU labeling and total cell count. This proliferation effect was abolished by the administration of MAG suggesting specificity. In addition, we demonstrate that sNgR-Fc is a potent activator for Notch1 and Notch1 antagonist reversed the effect of sNgR-Fc on NPC proliferation. Our results suggest that sNgR-Fc may modulate Nogo activity to induce NPC proliferation via the Notch pathway

Ontogenetic Development of Digestive Enzymes in Orange-Spotted Grouper (Epinephelus coioides, Hamilton, 1822) Larvae

The digestive physiology of orange-spotted grouper (Epinephelus coioides) was studied by assessing the specific and total activities of alkaline phosphatase, amylase, lipase, chymotrypsin, and trypsin, from hatching to 34 days post-hatching (DPH). From the onset of exogenous feeding, total alkaline phosphatase, amylase, lipase, and chymotrypsin activity fluctuated then sharply increased from 18 to 34 DPH. In contrast to other enzymes, the specific and total activity of trypsin remained at a low level from 1-26 DPH, and reached the maximum on 30 DPH. Digestive enzymes activity indicated that orange-spotted grouper were able to digest proteins, lipids, and carbohydrates at an early developmental stage. Results from the present study will improve understanding of the ontogeny of orange-spotted grouper during the larval stage, and provide a guide to hatchery feeding of this economically important fish

Predicting Suitable Environments and Potential Occurrences for Cinnamomum camphora (Linn.) Presl.

Global climate change has created a major threat to biodiversity. However, little is known about the habitat and distribution characteristics of Cinnamomum camphora (Linn.) Presl., an evergreen tree growing in tropical and subtropical Asia, as well as the factors influencing its distribution. The present study employed Maxent and a GARP to establish a potential distribution model for the target species based on 182 known occurrence sites and 17 environmental variables. The results indicate that Maxent performed better than GARP. The mean diurnal temperature range, annual precipitation, mean air temperature of driest quarter and sunshine duration in growing season were important environmental factors influencing the distribution of C. camphora and contributed 40.9%, 23.0%, 10.5%, and 7.2% to the variation in the model contribution, respectively. Based on the models, the subtropical and temperate regions of Eastern China, where the species has been recorded, had a high suitability for this species. Under each climate change scenario, the potential geographical distribution shifted farther north and toward a higher elevation. The predicted spatial and temporal distribution patterns of this species can provide guidance for the development strategies for forest management and species protection

Trauma does not accelerate neuronal degeneration in Fig4 insufficient mice

Fig4 null reduces phosphatidylinositol-3,5-diphosphate concentration and causes severe neuronal degeneration in both pale-tremor ( plt) mice and patients with Charcot-Marie-Tooth disease type 4J (CMT4J), an inherited condition with recessive mutations in FIG4. Our previous study shows that minor trauma is associated with an accelerated course of motor neuron degeneration in patients with CMT4J. Heterozygous loss of FIG4 function has been suggested to be a risk factor in developing sporadic amyotrophic lateral sclerosis. We therefore hypothesize that minor trauma may trigger or exacerbate motor neuron degeneration in mice with fig4 haploinsufficiency ( plt+/−). We have studied 18 wild-type and 18 plt+/− mice and created nerve injury by compressing the sciatic nerve. Outcomes in the mice were evaluated by nerve conduction study, Rotarod, and nerve morphology. No differences were found between wild-type and plt+/− mice. Taken together, our results demonstrate that haploinsufficiency of fig4 does not impose risks in rodents to develop neuronal degeneration in either naïve or traumatic conditions

Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall.

The herbaceous peony (Paeonia lactiflora Pall.) is widely cultivated as an ornamental, medicinal and edible plant in China. Drought stress can seriously affect the growth of herbaceous peony and reduce its quality. In our previous research, a significantly differentially expressed gene, PM19L, was obtained in herbaceous peony under drought stress based on transcriptome analysis, but little is known about its function. In this study, the first PM19L that was isolated in herbaceous peony was comprised of 910 bp, and was designated as PlPM19L (OP480984). It had a complete open reading frame of 537 bp and encoded a 178-amino acid protein with a molecular weight of 18.95 kDa, which was located in the membrane. When PlPM19L was transferred into tobacco, the transgenic plants had enhanced tolerance to drought stress, potentially due to the increase in the abscisic acid (ABA) content and the reduction in the level of hydrogen peroxide (H2O2). In addition, the enhanced ability to scavenge H2O2 under drought stress led to improvements in the enzyme activity and the potential photosynthetic capacity. These results combined suggest that PlPM19L is a key factor to conferring drought stress tolerance in herbaceous peony and provide a scientific theoretical basis for the following improvement in the drought resistance of herbaceous peony and other plants through genetic engineering technology