PO-127 Research progresses of exercise intervention methods in non- alcoholic fatty liver disease

Objective This review summarizes the effects of aerobic exercise (AE), resistance exercise (RE), and high-intensity interval training (HIIT) on nonalcoholic fatty liver disease (NAFLD).Discuss The main differences of intervention methods, intervention time, and intervention effects among the NAFLD people, To explore the targeting and dose-response relationship of different exercise models intervention in NAFLD.  Methods This paper uses the keywords such as Non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, NAFLD, NASH, NAFLD and aerobic training, aerobic exercise, resistance training, resistance exercise, high-intensity interval training, high-intensity interval exercise, high-intensity aerobic exercise, HIAT, HIIT, HIT, etc to search in the web of science、Pubmed database, the deadline is March 1, 2018. Inclusion criteria: (1) study design: randomized controlled trials; (2) study questions: effects of exercise on hepatic steatosis in patients with NAFLD; (3) type of exercise: AE, RE, and HIIT; (4) subjects: biochemical examination liver biopsy or ultrasound examination includes a combination of abdominal imaging and magnetic resonance imaging (MRI) imaging of the NAFLD population. Exclusion criteria: (1) non-original studies (2) meeting abstracts (3) did not provide sufficient experimental data (4) animal experiments (5) non-English literature. The search results showed that a total of 16 studies reported the effects of exercise on NAFLD populations.  Results (1) AE, RE and HIIT can reduce hepatic steatosis and improve liver histology in NAFLD people, but their intervention effects are different. AE stands out in reduce body weight,decreases insulin resistance (IR); RE stands out in reduce hepatic fat and increases muscle strength; HIIT has a significant effect in reducing hepatic fat and enhancing cardiovascular fitness. (2) the frequency, duration, and intervention period of AE and RE are similar; achieve the same or better intervention effect, HIIT only requires the 1/3 exercise time of the previous two. Conclusions RE may be more effective than AE in patients with poor cardiovascular fitness, sarcopenia, and NAFLD who are unable to tolerate or participate in AE; HIIT has certain advantages in the time-effect and dose-effect due to less exercise time and smaller amount of exercise, This is easy for the NAFLD people to accept, and it will facilitate long-term adherence in the future

Clinical characteristics and high risk factors of patients with Omicron variant strain infection in Hebei, China

ObjectiveThe Omicron variant has a weaker pathogenicity compared to the Delta variant but is highly transmissible and elderly critically ill patients account for the majority. This study has significant implications for guiding clinical personalized treatment and effectively utilizing healthcare resources.MethodsThe study focuses on 157 patients infected with the novel coronavirus Omicron variant, from December, 2022, to February, 2023. The objective is to analyze the baseline data, test results, imaging findings and identify risk factors associated with severe illness.ResultsAmong the 157 included patients, there were 55 cases in the non-severe group (all were moderate cases) and 102 cases in the severe group (including severe and critical cases). Infection with the Omicron variant exhibits significant differences between non-severe and severe cases (baseline data, blood routine, coagulation, inflammatory markers, cardiac, liver, kidney functions, Chest CT, VTE score, etc.). A multifactorial logistic regression analysis showed that neutrophil percentage >75%, eosinophil percentage <0.4%, D-dimer >0.55 mg/L, PCT >0.25 ng/mL, LDH >250 U/L, albumin <40 g/L, A/G ratio <1.2, cholinesterase<5100 U/L, uric acid >357 mole/L and blood calcium<2.11 mmol/L were the most likely independent risk factors for severe novel coronavirus infection.ConclusionAdvanced age, low oxygenation index, elevated neutrophil percentage, decreased eosinophil percentage, elevated PCT, elevated LDH, decreased albumin, decreased A/G ratio, elevated uric acid, decreased blood calcium, and elevated D-dimer are independent prognostic risk factors for non-severe patients progressing to severe illness. These factors should be closely monitored and actively treated to prevent or minimize the occurrence of severe illness

Evaluation of an identification method for the SARS-CoV-2 Delta variant based on the amplification-refractory mutation system

The Delta variant of SARS-CoV-2 dominated the COVID-19 pandemic due to its high viral replication capacity and immune evasion, causing massive outbreaks of cases, hospitalizations, and deaths. Currently, variant identification is performed mainly by sequencing. However, the high requirements for equipment and operators as well as its high cost have limited its application in underdeveloped regions. To achieve an economical and rapid method of variant identification suitable for undeveloped areas, we applied an amplification-refractory mutation system (ARMS) based on PCR for the detection of novel coronavirus variants. The results showed that this method could be finished in 90 min and detect as few as 500 copies/mL and not react with SARS-Coronavirus, influenza A H1N1(2009), and other cross-pathogens or be influenced by fresh human blood, α- interferon, and other interfering substances. In a set of double-blind trials, tests of 262 samples obtained from patients confirmed with Delta variant infection revealed that our method was able to accurately identify the Delta variant with high sensitivity and specificity. In conclusion, the ARMS-PCR method applied in Delta variant identification is rapid, sensitive, specific, economical, and suitable for undeveloped areas. In our future study, ARMS-PCR will be further applied in the identification of other variants, such as Omicron

Gender differences in self-care maintenance and its associations among patients with chronic heart failure

Objectives: To identify the gender differences in self-care maintenance and its associations among chronic heart failure patients using the Information-Motivation-Behavioral Skills model. Methods: Two hundred and ten patients (54.0% female) with chronic heart failure participated in this cross-sectional study. Self-care, knowledge of heart failure, social support and illness perception were measured using the Self-Care of Heart Failure Index, the questionnaire of heart failure knowledge, the Perceived Social Support Scale, and the Revised Illness Perception Questionnaire, respectively. Results: Mean scores for self-care maintenance were 51.4 ± 14.8 in men and 55.6 ± 14.1 in women (t = −2.066, P < 0.05). Associated factors of self-care maintenance were social support and self-care confidence in men and the knowledge of heart failure, self-care management and self-care confidence in women. The relationship between social support and self-care maintenance was meditated by self-care confidence in men, whereas the relationship between knowledge of heart failure and self-care maintenance was meditated by self-care management and self-care confidence in women. Conclusions: Self-care maintenance were inadequate in both genders with chronic heart failure. Interventions for enhancing social support and self-care confidence in men patients, and strengthening knowledge of heart failure, self-care management and self-care confidence in women patients, may facilitate self-care maintenance. Keywords: Heart failure, Illness perception, Knowledge, Self-care, Social suppor

Emotional and Behavioral Problems Among 3- to 5-Year-Olds Left-Behind Children in Poor Rural Areas of Hunan Province: A Cross-Sectional Study

The term left-behind children (LBC) refers to underage children who have been separated from their father/mother or parents for a long period of time. At present, there are few studies on the emotional and behavioral problems of three- to five-year-old LBC in poor rural areas of China. In this study, random cluster sampling was used to recruit caregivers of left-behind children (CLBC) aged three to five years in two poor rural areas in Hunan Province. General demographic data of LBC and their caregivers were collected via face-to-face questionnaires. Children&rsquo;s emotional and behavioral problems were assessed by the Strength and Difficulties Questionnaire (SDQ). Among 557 LBC, the prevalence of a total difficulties score in an abnormal/borderline range was 27.6&ndash;50.6%. The most common problem of LBC was hyperactivity, with a rate of 33.6%. Compared with boys, girls had more emotional problems (p &lt; 0.05) and fewer hyperactivity disorders (p &lt; 0.01). Factors related to the emotional and behavioral problems of LBC were the LBC&rsquo;s age, the number of sick people at home, and the CLBC&rsquo;s willingness to take care of the LBC. The detection rate of emotional and behavioral problems of three- to five-year-old LBC in poor rural areas is higher than that of children of the same age in urban areas and Western developed countries. There were gender differences in hyperactivity and emotional symptoms. Poor care will increase the risk of children&rsquo;s emotional and behavioral abnormalities

Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2

Background. Thermo-TRPs (temperature-sensitive transient receptor potential channels) belong to the TRP (transient receptor potential) channel superfamily. Emerging evidence implied that thermo-TRPs have been involved in regulation of cell fate in certain tumors. However, their distribution profiles and roles in melanoma remain incompletely understood. Methods. Western blot and digital PCR approaches were performed to identify the distribution profiles of six thermo-TRPs. MTT assessment was employed to detect cell viability. Flow cytometry was applied to test cell cycle and apoptosis. Calcium imaging was used to determine the function of channels. Five cell lines, including one normal human primary epidermal melanocytes and two human malignant melanoma (A375, G361) and two human metastatic melanoma (A2058, SK-MEL-3) cell lines, were chosen for this research. Results. In the present study, six thermo-TRPs including TRPV1/2/3/4, TRPA1, and TRPM8 were examined in human primary melanocytes and melanoma cells. We found that TRPV2/4, TRPA1, and TRPM8 exhibited ectopic distribution both in melanocytes and melanoma cells. Moreover, activation of TRPV2 and TRPV4 could lead to the decline of cell viability for melanoma A2058 and A375 cells. Subsequently, activation of TRPV2 by 2-APB (IC50 = 150 μM) induced cell necrosis in A2058 cells, while activation of TRPV4 by GSK1016790A (IC50 = 10 nM) enhanced apoptosis of A375 cells. Furthermore, TRPV4 mediated cell apoptosis of melanoma via phosphorylation of AKT and was involved in calcium regulation. Conclusion. Overall, our studies revealed that TRPV4 and TRPV2 mediated melanoma cell death via channel activation and characterized the mechanism of functional TRPV4 ion channel in regulating AKT pathway driven antitumor process. Thus, they may serve as potential biomarkers for the prognosis and are targeted for the therapeutic use in human melanoma

Mechanistic study of incipient corrosion for nuclear grade lean-Cr FeCrAl alloys in a simulated PWR environment

This study systematically investigated the incipient corrosion behavior of Fe-13Cr-4.5Al (wt%) and lean-Cr (7–10 wt%) with 5Al (wt%) FeCrAl alloy with a timescale of 4 months under simulated PWR conditions (18.6 MPa and 360 °C) in an autoclave system. The corrosion induced two types of structure of oxide scale. FeCrAl alloys corroded for 3 months exhibited a duplex structure containing outer oxide particles and an inner Cr-rich spinel. During the corrosion test lasting 3–4 months, a triplex structure containing outer oxide particles, an inner compact Cr-rich spinel layer, and a porous spinel layer was observed. Moreover, Cr enriched at the periphery of outer oxides, indicating the formation of FeCr2O4 spinel oxide