An Empirical Study of User Acceptance on Medical and Health Website Based on UTAUT

With the rapid development of Internet, more and more people got medical information through Medical and Health Website. However, Medical and Health Website is still in infancy, its social influence is not significant, and this new Internet technology has not been widely accepted in our country. Most scholars mainly concentrate on technology and management institution when researching the Medical and Health Website. In order to study user’s behavioral intention and use behavior on Medical and Health website, a theoretical model is built based on Unified Theory of Acceptance and Use of Technology (UTAUT) with the consumer perceived risk theory and perceived cost. The questionnaire is designed according to the theory model. Then, the data is analyzed by descriptive statistics, reliability and validity and multiple linear regression. The results indicate that people’s use behavior is positive influenced by behavioral intention and effort expectancy. On the contrary, perceived cost has negative influence on use behavior. Perceived risk has negative influence on behavioral intention. Gender and experience have moderating effects on behavioral intention. However, it is not verified that social influence has positive influence on behavioral intention. At last, this paper provides some countermeasures and suggestions to promote the development of Medical and Health website

PO-115 Effects of Phosphatidylserine on Mental States in Elite Shooters

Objective The purpose of this study was to examine effects of a short-term oral supplementation of PS on biochemical stress indicators, whole brain neurotransmitters, mood states, and sleep quality in elite shooters. Methods Eighteen shooters including 9 males and 9 females participated in the study.  They were randomly assigned into either of the three groups: 1) those who ingested PS at 400 mg∙day-1 (n=6) (PS-400); 2) those who ingested PS at 800 mg∙day-1 (n=6) (PS-800); 3) those who with no supplement (n=6) (CON). PS supplementation lasted for 14 days and was administered in a double-blinded fashion. Within a week prior to the supplementation, serial venous blood samples were taken for measuring serum levels testosterone (T), cortisol (C), blood urea nitrogen (BUN), and creatine kinase (CK).  Subjects also completed the Profile of Mood States (POMS) scale and the Pittsburgh Sleep Quality Index (PSQI) questionnaire and undertook electroencephalogram that determined activation values of six neurotransmitters in whole brain, including inhibition of medium (INH), five hydroxy-tryptamine (5-HT), acetylcholine (ACH), dopamine (DA), norepinephrine (NE), and excited medium (EXC). Subjects repeated the same testing protocols and all variables were measured again after 14 days of supplementation. Results No between-group differences in all variables were observed at the baseline prior to the start of supplementation.  After supplementation, both C and C increase were lower (P=0.025, P=0.016, respectively) in PS 800 than CON, while no significant differences in C and C increase were seen between PS-400 and CON and between PS-800 and PS-400.  5-HT and DA were higher  (P=0.049, P=0.019, respectively) in PS-800 than CON, while no differences in 5-HT and DA were observed between PS-400 and CON and between PS-800 and PS-400.  All neurotransmitters were increased by supplementation in PS-800, with ACH and DA reaching statistical significance (P=0.050,P=0.029, respectively).  A synchronized trend of INH, 5-HT, ACH, DA，NE, and EXC were observed in a few brain regions (P<0.05).  PS Supplementation decreased panic score of POMS following both PS-400 and PS-800, with a greater decrease seen in PS-800.  The panic score post-supplementation was lower in PS-800 than PS-400 (P=0.016) or CON (P=0.027).  Although results of PSQI indicated an improved sleep quality following supplementation in both PS-400 and PS-800, these improvements did not reach statistical significance and no differences in PSQI were seen across the three groups. Conclusions It appears that supplementation with phosphatidylserine at 800 mg∙day-1 for 14 days can reduce stress hormones, modulate central neurotransmitters, and mitigate negative emotions, thereby alleviating stress levels among elite shooters

Genome-wide identification, expression and function analysis of the MTP gene family in tulip (Tulipa gesneriana)

Currently, soil heavy metal contamination is a severe issue, particularly with Cd pollution. The metal tolerance protein (MTP) proteins, as plant divalent cation transporters, play a crucial role in the transport and tolerance of heavy metals in plants. This study conducted comprehensive identification and characterization of the MTP gene family in the tulip. A total of 11 TgMTP genes were identified and phylogenetically classified into three subfamilies. Conserved motif and gene structure analyses unveiled commonalities and variations among subfamily members. Expression profiling demonstrated several TgMTPs were markedly upregulated under Cd exposure, including the TgMTP7.1. Heterologous expression in yeast validated that TgMTP7.1 could ameliorate Cd sensitivity and enhance its tolerance. These results provide primary insights into the MTP gene family in tulip. Phylogenetic relationships and functional analyses establish a framework for elucidating the transporters and molecular mechanisms governing Cd accumulation and distribution in tulip. Key TgMTPs identified, exemplified by TgMTP7.1, may illuminate molecular breeding efforts aimed at developing Cd-tolerant cultivars for the remediation of soil Cd contamination

OR-011 Effect of Aerobic and Resistance Exercise on TGF-β1/Smad3 Signal Pathway and Collagen in Skeletal Muscle of Aging Mice

Objective The purpose of this study was to investigate the effects of TGF-β 1/Smad3  signaling pathway and its downstream factor CTGF in collagen deposition and its molecular mechanism. And then it explored further the effect of exercise on the TGF-β1/Smad3 signaling pathway and collagen deposition in skeletal muscle. Therefore, it is expected to provide alternative exercise intervention approaches for skeletal muscle diseases, which are caused by age-related changes of collagen, and to provide new research perspectives for skeletal muscle satellite cell activation and skeletal muscle regeneration. Methods 21 male BALB/c mice were normally raised from 4 weeks to 36 weeks under standard conditions. The mice was divided randomly into three groups, including: group C, the quiet control group; Group A, the aerobics training group, received nine weeks of treadmill training without loading; And the group R, the resistance training group, received nine weeks of climbing training with loading. The body weight and limb grip of the mice were measured on regularly during the experiment. After 24 hours of the last intervention experiment, the mice were weighed and then executed by dislocating the cervical spine. The quadriceps were taken. Real-time PCR technology was used to detect the mRNA levels of TGF-β1, TβR I, Smad 3, CTGF, Pax7, COL1 and COL3. Western blotting technique was used to detect the protein levels of TGF-β1, Smad3, P-Smad3 , CTGF, COL1, COL3, Pax7 and MyoD . The deposition of collagen in the quadriceps muscle tissue of mice was detected by Sirius red staining. And the localization and expressions of COL1 and Pax7 in the quadriceps of mice were demonstrated by immunohistochemistry and immunofluorescence technology respectively. Results (1)       Compared with group C, the weight of mice in group A was significantly increased (P<0.05), and the ratio of the wet weight of the quadriceps and the body weight of the mice increased significantly (P<0.05), while there was no significant change on the limbs relative grip strength. Compared with group C, the body weight of mice in group R showed a certain degree of increase but no significant difference, the ratio between the wet weight of the quadriceps and the body weight of the mice was significantly increased (P<0.01), and the limbs relative grip strength was significantly increased (P<0.05). (2)     Compared with group C, there was no significant difference in the mRNA and protein expression of COL1 and COL3 in the quadriceps of mice in group A, and there was no significant change in collagen volume fraction. Compared with group C, the mRNA and protein expression of COL1 and COL3 of the quadriceps in group R were significantly decreased (P<0.05), and collagen volume fraction significantly reduced (P<0.05), and collagen deposition decreased. (3)     Compared with group C, the mRNA level of CTGF and the protein level of TGF-β1 and CTGF in quadriceps tissues of mice in group A were significantly decreased (P<0.05). While the protein levels of Smad3 and p-Smad3 and the ratio of Smad3 and p-Smad3 had no significant change. Group R is compared with group C, the mRNA level of TGF-β1, TβR I and CTGF in quadriceps were significantly decreased (P<0.05); the mRNA levels of Smad3 and the protein levels of TGF-β1 and p-Smad3 were significantly decreased (P<0.01); and the protein levels of Smad3 and CTGF and the ratio of Smad3 and p-Smad3 were also significantly decreased (P<0.05). (4)     Compared with group C, the mRNA and protein expression of Pax7, and the protein expression of MyoD in the quadriceps of group A showed no significant difference. But group R is compared to group C, the expression of Pax7 mRNA in the quadriceps was significantly increased (P<0.01), and the expression of Pax7 protein was also significantly increased (P<0.05), while the protein expression of MyoD did not change significantly. Compared with group A, the mRNA levels of Pax7 in the quadriceps of the R group was significantly increased (P<0.05), but the protein expression of Pax7 and MyoD showed no significant change. Conclusions (1)     Through 9 week resistance or aerobic exercise training, skeletal muscle mass index in mice increased significantly; and the resistance exercise training can improve the limbs relative grip strength to prevent sacorpenia. (2)     9 week resistance exercise training can inhibit TGF-β1/Smad3 signaling pathway, affect the gene expression of COL1 and COL3, inhibit collagen synthesis, and improve collagen deposition. (3)     9 weeks of resistance exercise training can effectively promote Pax7 gene expression, activate skeletal muscle satellite cells and promote its proliferation. (4)     The effect of 9 week of resistance exercise training on the improvement of skeletal muscle mass, strength, collagen deposition and the activation of satellite cells was significantly better than that of aerobic exercise

CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation

Osteoporosis is a chronic age-related disease. During aging, bone marrow-derived mesenchymal stem cells (BMSCs) display increased adipogenic, along with decreased osteogenic, differentiation capacity. The aim of the present study was to investigate the effect of calcitonin gene-related peptide (CGRP) on the osteogenic and adipogenic differentiation potential of BMSC-derived osteoblasts. Here, we found that the level of CGRP was markedly lower in bone marrow supernatant from aged mice compared with that in young mice. In vitro experiments indicated that CGRP promoted the osteogenic differentiation of BMSCs while inhibiting their adipogenic differentiation. Compared with vehicle-treated controls, aged mice treated with CGRP showed a substantial promotion of bone formation and a reduction in fat accumulation in the bone marrow. Similarly, we found that CGRP could significantly enhance bone formation in ovariectomized (OVX) mice in vivo. Together, our results suggested that CGRP may be a key regulator of the age-related switch between osteogenesis and adipogenesis in BMSCs and may represent a potential therapeutic strategy for the treatment of age-related bone loss

Reliability and validity of the Chinese version of the Athens insomnia scale for non-clinical application in Chinese athletes

PurposeThis study aimed to revise and examine the reliability and validity of the Chinese version of the Athens Insomnia Scale for Non-clinical Application (AIS-NCA) among Chinese athletes. Additionally, the study tested the scale in non-athlete individuals with similar sleep management practices to further analyze its cultural specificity among Chinese athletes and make preliminary inferences about its applicability in other Chinese populations.MethodsFour hundred twenty-six Chinese professional athletes and 779 high school students participated in this research. Both athletes and students were divided into two parallel groups for exploratory and confirmatory factor analyses. Additionally, three athlete samples and one student sample were established for reliability and validity assessments. Among athletes, the Pittsburgh Sleep Quality Index, the Epworth Sleepiness Scale, the Athlete Sleep Screening Questionnaire, and the Warwick-Edinburgh Mental Well-Being Scale were employed to evaluate convergent and discriminant validity. Re-test reliability was evaluated at intervals of 1 and 2 weeks. In the case of students, convergent and discriminant validity were tested using the Pittsburgh Sleep Quality Index and the General Self-Efficacy Scale, with re-test reliability assessed at two-week intervals.ResultsThe Chinese version of the AIS-NCA consists of six items, categorized into two dimensions: sleep problems and daytime functioning. This structure explained 65.08% (athletes) and 66.22% (students) of the variance. Confirmatory factor analysis revealed good model fit, with values of χ2/df = 2.217, CFI = 0.975, AGFI = 0.929, TLI = 0.953, and RMSEA = 0.076 among athletes, and χ2/df = 3.037, CFI = 0.979, AGFI = 0.947, TLI = 0.961, and RMSEA = 0.072 among students. The scale demonstrated a reasonable degree of measurement invariance. The overall scale and two subscales exhibited strong reliability and validity among athletes. Similar results in terms of reliability and validity were also observed within the student sample.ConclusionThe Chinese version of the AIS-NCA shows promise as an assessment tool for evaluating the sleep quality of Chinese athletes. It effectively captures both sleep-related concerns and daytime functionality within the athlete population. The scale demonstrates solid reliability and validity in professional athletes and holds potential for application across various other demographic groups in China

Ginsenoside 20(S)-Rg3 Prevents PKM2-Targeting miR-324-5p from H19 Sponging to Antagonize the Warburg Effect in Ovarian Cancer Cells

Background/Aims: The Warburg effect is one of the main metabolic features for cancers, with long non-coding RNA (lncRNA) being involved as a class of crucial regulators. Our previous studies have shown that ginsenoside 20(S)-Rg3, an active saponin monomer extracted from red ginseng, inhibits the Warburg effect in ovarian cancer cells. However, the detailed lncRNA regulatory network modulated by 20(S)-Rg3 to prevent the Warburg effect in ovarian cancer cells has not been explored. Methods: High-throughput sequencing was used to screen out the differentially expressed lncRNAs between 20(S)-Rg3-treated and non-treated SKOV3 cells. The levels of lncRNA H19 and miR-324-5p were manipulated in SKOV3 and A2780, and the glucose consumption, lactate production and PKM2 protein level were detected. Dual-luciferase reporter assay and RIP were utilized to verify the direct binding of H19 to miR-324-5p and miR-324-5p to PKM2. Cell proliferation was examined by CCK8 and colony formation assay. Nude mice subcutaneous xenograft tumor models were established to evaluate the impact of miR-324-5p on tumor growth in vivo. Results: 20(S)-Rg3 downregulated 67 lncRNAs, and H19 was one of the most decreased lncRNAs. Suppression of H19 by siRNA transfection reduced glucose consumption, lactate production and PKM2 expression in ovarian cancer cells, while H19 overexpression in 20(S)-Rg3-treated ovarian cancer cells enhanced glucose consumption, lactate production and PKM2 expression. Dual-luciferase reporter assay and RIP results showed that H19 directly bound to miR-324-5p. Dual-luciferase reporter assay showed that miR-324-5p directly targeted PKM2, and miR-324-5p negatively regulated glucose consumption and lactate production in ovarian cancer cells. miR-324-5p overexpression inhibited cell proliferation in vitro and in vivo. Conclusion: Our study revealed that 20(S)-Rg3 blocked the competitive inhibition of H19 on miR-324-5p, which enhanced the suppression of miR-324-5p on PKM2 and therefore inhibited the Warburg effect and repressed tumorigenesis. In a word, 20(S)-Rg3 inhibited the Warburg effect in ovarian cancer cells via H19/miR-324-5p/PKM2 pathway