Research on the Impact Mechanism of the Ambidextrous Innovation of Public Administrators

This study draws on the literature of social cognitive theory to examine the impact of leadership self-efficacy on the ambidextrous innovation of public administrators. Afterwards, this study introduces perspective taking as a moderator and then discusses the different moderating effects of cognitive perspective taking and affective perspective taking. Findings show that leadership self-efficacy has a significant positive effect on ambidextrous innovation; cognitive perspective taking and affective perspective taking play important roles in the relationship between leadership self-efficacy and ambidextrous innovation; the interaction of affective perspective taking and leadership self-efficacy is not conducive to the realization of the ambidextrous innovation of public administrators. This study is a pioneering endeavor in the field of innovation research at the individual level in Chinese public sector, thereby providing important implications for public administrators to stimulate their ambidextrous innovation. Keywords: public administrators, ambidextrous innovation, leadership self-efficacy, cognitive perspective taking, affective perspective takin

Network Centrality and Public Service Motivation: The Medium of Organizational Commitment

Based on social network theory, this paper studies the mechanism of betweenness centrality on public service motivation and how this influence is mediated by organizational commitment. The theory of public service motivation, as a product of the crisis of Western social government, has attracted a lot of academic interest because of its completely different ideas. In the subsequent development, it has become a parallel with Weber's bureaucratic theory, representative bureaucracy, and public choice theory. One of the four theoretical schools that describe bureaucratic behavior. As an emerging topic in the field of public management research, in-depth study of public service motivation not only helps to select a qualified civil service, but also helps to better improve public service motivation in the public sector and accelerate service-oriented government construction pace. Through a questionnaire survey of 206 public sector members, the empirical analysis shows that the higher the betweenness are, the more motivated individuals are. In addition, the organization promises to play an intermediary role between the betweenness centrality and public service motivation. The article's research conclusions, from the point of view of social network theory, enrich the angle and strength of interpretation of public service motivation, and also provide new ideas for the future public sector to further enhance the public service motivation of the organization members. Keywords:public service motivation, betweenness centrality, organizational commitmen

Expression, Purification and Activity Study of Amuc_1100

Objective: To construct prokaryotic expression vector of Amuc_1100 gene, the ELP (ELSTIN-LIKE PROTEIN) is used as purification label to express fusion with the target protein and detect the biological activity of recombinant protein at cell level and animal level. Methods: the recombinant plasmid pET28a-Amuc_1100-ELP is designed and synthesized, and transformed into e. coli competent cell bl21 (de3) to induce expression. The recombinant egg was isolated and purified by the reversible phase change cycling (ITC) characteristic of ELP10 white. At the cellular level, PBMCs cells from mice were extracted to detect the expression level of IL-10. At the animal level, obese mice induced by gastric gavage with high fat detected the improvement effect of the protein on mouse metabolism. Results: The recombinant protein with the expected molecular weight of 60 kDa was expressed by E.coli expression system. After circulating through ITC, the recombinant protein with high purity was obtained. The protein increased the expression level of IL-10 in PBMCs cells, and significantly improved the obesity and metabolic disorders induced by high-fat diet in mice. Conclusion: The recombinant protein expression plasmid pET28a-Amuc_1100-ELP was successfully constructed. After expression and purification, the recombinant protein expression plasmid has high purity and has 15 biologically active recombinant protein. This study laid a foundation for the industrial production and clinical application of Amuc_1100

The clinical value of ultrasound in assessing ovarian strangulation in female infants and toddlers with ovarian hernia

ObjectiveTo explore the clinical value of ultrasound examination in evaluating the presence of ovarian torsion in female infants with inguinal ovarian hernia.MethodsWe conducted a retrospective analysis of 91 cases of ovarian hernia diagnosed by ultrasonography at our institution. Among them, 6 cases were identified as ovarian strangulation, while 85 cases were classified as non-ovarian strangulation. All cases underwent high-frequency ultrasound examination. We analyzed whether there were differences in the associated factors between the two groups and compared the disparities in the urgency of surgery between the two groups.ResultsSignificant differences were observed between the ovarian strangulation group and the non- strangulation group in terms of ovarian volume, ovarian blood flow, and the presence of incarceration. The need for emergency surgery was significantly associated with the presence of incarceration and ovarian torsion.ConclusionUltrasound has good clinical value in diagnosing ovarian hernia and determining the presence of ovarian strangulation. It can assist clinical physicians in determining the timing of surgery for children with ovarian hernia