Comparative analysis of microRNA profiles between wild and cultured Haemaphysalis longicornis (Acari, Ixodidae) ticks

The miRNA profiles of a Haemaphysalis longicornis wild-type (HLWS) and of a Haemaphysalis longicornis cultured population (HLCS) were sequenced using the Illumina Hiseq 4000 platform combined with bioinformatics analysis and real-time polymerase chain reaction (RT-PCR). A total of 15.63 and 15.48 million raw reads were acquired for HLWS and HLCS, respectively. The data identified 1517 and 1327 known conserved miRNAs, respectively, of which 342 were differentially expressed between the two libraries. Thirty-six novel candidate miRNAs were predicted. To explain the functions of these novel miRNAs, Gene Ontology (GO) analysis was performed. Target gene function prediction identified a significant set of genes related to salivary gland development, pathogen-host interaction and regulation of the defence response to pathogens expressed by wild H. longicornis ticks. Cellular component biogenesis, the immune system process, and responses to stimuli were represented at high percentages in the two tick libraries. GO enrichment analysis showed that the percentages of most predicted functions of the target genes of miRNA were similar, as were certain specific categories of functional enhancements, and that these genes had different numbers and specific functions (e.g., auxiliary transport protein and electron carrier functions). This study provides novel findings showing that miRNA regulation affects the expression of immune genes, indicating a considerable influence of environment-induced stressful stimulation on immune homeostasis. Differences in the living environments of ticks can lead to differences in miRNAs between ticks and provide a basis and a convenient means to screen for genes encoding immune factors in ticks

Molecular Evidence of Bartonella melophagi in Ticks in Border Areas of Xinjiang, China

Bartonella are gram-negative intracellular bacteria; certain species of Bartonella can cause diseases in mammals and humans. Ticks play a major role in the transmission of Bartonella. Xinjiang is the largest province in China according to land area and has one-third of the tick species in China; the infection rate of Bartonella in ticks in the Xinjiang border areas has not been studied in detail. Therefore, this study investigated tick infections by Bartonella in Xinjiang border areas, and the purpose of the study was to fill in gaps in information regarding the genetic diversity of tick infections by Bartonella in Xinjiang. We tested 1,549 tick samples from domestic animals (sheep and cattle) for Bartonella using ribC-PCR. Positive samples from the ribC-PCR assay for Bartonella spp. were further subjected to PCR assays targeting the ITS, rpoB and gltA genes followed by phylogenetic analyses. Bartonella DNA was detected in 2.19% (34/1,549) of tick samples, and the ITS, rpoB and gltA genes of ribC gene-positive samples were amplified to identify nine samples of Bartonella melophagi. In this study, molecular analysis was used to assess the presence and genetic diversity of B. melophagi in ticks collected from sheep and cattle from Xinjiang, China. This study provides new information on the presence and identity of B. melophagi in ticks from sheep and cattle

Ultrawideband and high-efficient polarization conversion metasurface based on multi-resonant element and interference theory

In this work, an ultrawideband and high-efficient polarization conversion metasurface (PCM) is proposed, which can efficiently convert linearly polarized waves into cross-polarized waves in an ultra-wide frequency range. The unit cell of the proposed PCM is composed of two pairs of L-shaped metallic patches covered by a dielectric superstrate and an air-based substrate attached with a metallic ground. The PCM has an operating band from 3.37 to 22.07 GHz with the polarization conversion ratio (PCR) over 90% under the normal incidence, which the ratio bandwidth (fH/fL) is 6.5:1. The PCR can achieve 100% at seven resonant frequencies. The equivalent circuit model is analyzed to explain the fundamental cause of the PCM’s multi-resonance and polarization conversion behaviors. In addition, all possible near-field interactions among the resonator, the superstrate, and the ground sheet can be accurately calculated using interference theory, which reveals the underlying physical mechanisms of the multi-resonance metasurface. The theoretical calculated, numerically simulated, and measured results are in good agreement. Compared to other PCMs, the proposed PCM has a simple geometry structure but an ultrawideband and high PCR property.This article is published as Yu, Hang, Xiaoyu Wang, Jianxun Su, Meijun Qu, Qingxin Guo, Zengrui Li, and Jiming Song. "Ultrawideband and high-efficient polarization conversion metasurface based on multi-resonant element and interference theory." Optics Express 29, no. 22 (2021): 35938-35950. DOI: 10.1364/OE.440542. © 2021 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.Posted with permission

Laboratory Experiment on Formation of MICP Horizontal Seepage-Reducing Body in Confined Aquifer for Deep Excavation

Using a soil improvement biotechnology based on microbial-induced carbonate precipitation (MICP) to form a horizontal water barrier in foundation pit dewatering can not only achieve a good dewatering control effect, but also control the cost and obtain good economic and environmental benefits. In this study, taking the Shanghai layer ⑨ sand as the research object, the solution injected into the layer ⑨ sand by MICP grouting technology includes bacterial solution (Sporosarcina pasteurii) and cementation solution (urea and CaCl2 solution). The migration mechanism of the bacterial solution and cementation solution in groundwater was analyzed through batch experiment and column test, and the mathematical model of migration was established. The dynamic adsorption characteristics of the S. pasteurii, Ca2+, and urea were obtained by dynamic adsorption test. Through isothermal adsorption test, the Freundlich isotherm was used to describe the isothermal adsorption of S. pasteurii onto fine sand, and the partition coefficient of the S. pasteurii was found to be 1.5 × 10−7 L/mg. The Langmuir isotherm can be used truly reflect the isothermal adsorption of Ca2+ onto fine sand. The distribution coefficient of the Ca2+ was 7 × 10−8 L/mg. The maximum adsorption capacity of the solute was 2404.8 mg/kg. The adsorption capacity of the urea was very small. According to the sand column test, the vertical dispersion was 11.5 cm. Due to the obvious size effect of dispersion, the sensitivity of dispersion in actual working conditions should be analyzed in a subsequent numerical simulation. The determined solute transport parameters provide support for the further study of the numerical simulation of the solute transport process of the HSRB formed by MICP grouting technology and provide a basis for further field application

Numerical Simulation of Forming MICP Horizontal Seepage Reducing Body in Confined Aquifer for Deep Excavation

The drawdown outside of a deep foundation pit has to be controlled during excavation. However, the vertical curtain cannot cutoff a deep and thick confined aquifer during deep excavation. In this study, a microbial-induced carbonate precipitation (MICP) horizontal seepage reducing body (HSRB) was proposed to control drawdown combined with a partially penetrating curtain. MICP HSRB is formed by using the seepage field generated by the recharge wells to drive the migration of a Sporosarcina pasteurii solution, stationary solution, and cementation solution into the deep confined aquifer. The migration of each solution was numerically simulated to study the HSRB formation process. The influence of different factors on solute migration was studied. The results show that the solutes in the fixed fluid and cementation fluid can reach the area under the driving of the seepage field, which proves that MICP HSRB can be formed. The calcium ions and urea in the cementation solution are more likely to migrate to the designated area than the bacterial solution. Increasing the injection rate of bacterial solution and adding recharge wells both made the bacterial solution migrate more quickly to the designated area. In the case of multiple grouting, the solute migration in the later stage will be hindered by the plugging of pores caused by calcium carbonate generated in the earlier stage. Therefore, different grouting methods need to be designed to drive the seepage field so that the solute injected in the later stage can continue to migrate. The MICP HSRB grouting technology can be used in foundation pit dewatering, providing reference for similar engineering

Numerical Simulation of Forming MICP Horizontal Seepage Reducing Body in Confined Aquifer for Deep Excavation

The drawdown outside of a deep foundation pit has to be controlled during excavation. However, the vertical curtain cannot cutoff a deep and thick confined aquifer during deep excavation. In this study, a microbial-induced carbonate precipitation (MICP) horizontal seepage reducing body (HSRB) was proposed to control drawdown combined with a partially penetrating curtain. MICP HSRB is formed by using the seepage field generated by the recharge wells to drive the migration of a Sporosarcina pasteurii solution, stationary solution, and cementation solution into the deep confined aquifer. The migration of each solution was numerically simulated to study the HSRB formation process. The influence of different factors on solute migration was studied. The results show that the solutes in the fixed fluid and cementation fluid can reach the area under the driving of the seepage field, which proves that MICP HSRB can be formed. The calcium ions and urea in the cementation solution are more likely to migrate to the designated area than the bacterial solution. Increasing the injection rate of bacterial solution and adding recharge wells both made the bacterial solution migrate more quickly to the designated area. In the case of multiple grouting, the solute migration in the later stage will be hindered by the plugging of pores caused by calcium carbonate generated in the earlier stage. Therefore, different grouting methods need to be designed to drive the seepage field so that the solute injected in the later stage can continue to migrate. The MICP HSRB grouting technology can be used in foundation pit dewatering, providing reference for similar engineering

Application of a Simplified Method for Estimating Perfusion Derived from Diffusion-Weighted MR Imaging in Glioma Grading

Purpose: To evaluate the feasibility of a simplified method based on diffusion-weighted imaging (DWI) acquired with three b-values to measure tissue perfusion linked to microcirculation, to validate it against from perfusion-related parameters derived from intravoxel incoherent motion (IVIM) and dynamic contrast-enhanced (DCE) magnetic resonance (MR) imaging, and to investigate its utility to differentiate low- from high-grade gliomas.Materials and Methods: The prospective study was approved by the local institutional review board and written informed consent was obtained from all patients. From May 2016 and May 2017, 50 patients confirmed with glioma were assessed with multi-b-value DWI and DCE MR imaging at 3.0 T. Besides conventional apparent diffusion coefficient (ADC0,1000) map, perfusion-related parametric maps for IVIM-derived perfusion fraction (f) and pseudodiffusion coefficient (D*), DCE MR imaging-derived pharmacokinetic metrics, including Ktrans, ve and vp, as well as a metric named simplified perfusion fraction (SPF), were generated. Correlation between perfusion-related parameters was analyzed by using the Spearman rank correlation. All imaging parameters were compared between the low-grade (n = 19) and high-grade (n = 31) groups by using the Mann-Whitney U test. The diagnostic performance for tumor grading was evaluated with receiver operating characteristic (ROC) analysis.Results: SPF showed strong correlation with IVIM-derived f and D* (ρ = 0.732 and 0.716, respectively; both P < 0.001). Compared with f, SPF was more correlated with DCE MR imaging-derived Ktrans (ρ = 0.607; P < 0.001) and vp (ρ = 0.397; P = 0.004). Among all parameters, SPF achieved the highest accuracy for differentiating low- from high-grade gliomas, with an area under the ROC curve value of 0.942, which was significantly higher than that of ADC0,1000 (P = 0.004). By using SPF as a discriminative index, the diagnostic sensitivity and specificity were 87.1% and 94.7%, respectively, at the optimal cut-off value of 19.26%.Conclusion: The simplified method to measure tissue perfusion based on DWI by using three b-values may be helpful to differentiate low- from high-grade gliomas. SPF may serve as a valuable alternative to measure tumor perfusion in gliomas in a noninvasive, convenient and efficient way

Load-Damping Characteristic Control Method in an Isolated Power System With Industrial Voltage-Sensitive Load

Load-damping characteristic models the load response to the frequency deviation, which has important impact on system frequency response. The load-damping coefficient is normally considered as a constant, obtained from operational experiences in large-scale power systems or from detailed load models in isolated power systems. An industrial isolated power system for aluminum production driven by coal-fired power and large scale wind power is studied in this paper. Since the electrolytic aluminum load is driven by direct current, it is one type of voltage-sensitive load and does not respond to the frequency deviation. This paper proposes a load-damping characteristic control method for such isolated industrial power system with voltage-sensitive load. To decrease the maximum frequency deviation during transient process, a time-varying load-damping coefficient control scheme is presented. Simulation is done on real-time digital simulator (RTDS) and the results verify the effectiveness of the proposed control method

Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques

Abstract Background Noninvasive cardiovascular magnetic resonance (CMR) techniques including arterial spin labeling (ASL), blood oxygenation level-dependent (BOLD), and intravoxel incoherent motion (IVIM), are capable of measuring tissue perfusion-related parameters. We sought to evaluate and compare these three CMR techniques in characterizing skeletal muscle perfusion in lower extremities and to investigate their abilities to diagnose and assess the severity of peripheral arterial disease (PAD). Methods Fifteen healthy young subjects, 14 patients with PAD, and 10 age-matched healthy old subjects underwent ASL, BOLD, and IVIM CMR perfusion imaging. Healthy young and healthy old participants were subjected to a cuff-induced ischemia experiment with pressures of 20 mmHg and 40 mmHg above systolic pressure during imaging. Perfusion-related metrics, including blood flow, T2* relaxation time, perfusion fraction f, diffusion coefficient D, and pseudodiffusion coefficient D*, were measured in the anterior, lateral, soleus, and gastrocnemius muscle groups. Friedman, Mann-Whitney, Wilcoxon signed rank, and Spearman rank correlation tests were used for statistical analysis. Results In cases of significant differences determined by the Friedman test (P  0.05). Blood flow and T2* values showed significant positive correlations with transcutaneous oxygen pressure measurements (ρ = 0.465 and 0.522, respectively; both P ≤ 0.001), while f values showed a significant negative correlation in healthy young subjects (ρ = − 0.351; P = 0.018). T2* was independent of age in every muscle group. T2* values were significantly decreased in PAD patients compared with healthy old subjects and severe PAD patients compared with mild-to-moderate PAD patients (all P < 0.0125). Significant correlations were found between T2* and ankle–brachial index values in all muscle groups in PAD patients (ρ = 0.644–0.837; all P < 0.0125). Other imaging parameters failed to show benefits towards the diagnosis and disease severity evaluation of PAD. Conclusions ASL, BOLD, and IVIM provide complementary information regarding tissue perfusion. Compared with ASL and IVIM, BOLD may be a more reliable technique for assessing PAD in the resting state and could thus be applied together with angiography in clinical studies as a tool to comprehensively assess microvascular and macrovascular properties in PAD patients