11,354 research outputs found
Rank-dependent deactivation in network evolution
A rank-dependent deactivation mechanism is introduced to network evolution.
The growth dynamics of the network is based on a finite memory of individuals,
which is implemented by deactivating one site at each time step. The model
shows striking features of a wide range of real-world networks: power-law
degree distribution, high clustering coefficient, and disassortative degree
correlation.Comment: 5 pages, 5 figures, RevTex
The consistency of estimator under fixed design regression model with NQD errors
In this article, basing on NQD samples, we investigate the fixed design
nonparametric regression model, where the errors are pairwise NQD random
errors, with fixed design points, and an unknown function. Nonparametric
weighted estimator will be introduced and its consistency is studied. As
special case, the consistency result for weighted kernel estimators of the
model is obtained. This extends the earlier work on independent random and
dependent random errors to NQD case
Mesenchymal stem cells overexpressing MiR-126 enhance ischemic angiogenesis via the AKT/ERK-related pathway
Background: This study was designed to examine whether transplantation of mesenchymal stem
cells (MSCs) overexpressing miR-126 enhances angiogenesis in the infarcted myocardium of mice.
Methods: MSCs were harvested from mice using density gradient centrifugation and adherent
culture. MSCs were transfected with lentiviral vectors carrying mature miR-126. Mice
models of myocardial infarction were established by ligation of coronary artery. The ligated
animals were randomly divided into three groups (15 in each) and after two weeks, were
intramyocardially injected at the heart infarct zone with miR-126-transfected MSCs (the miR-126-MSCs group), MSCs (the MSCs group), or medium (the PBS group). Six weeks later,
histological study and echocardiographic assessment were performed.
Results: Capillary density of the infarcted region was significantly improved in the miR-126-
MSCs group compared to the MSC group and the PBS group (both p < 0.01). Western blot
showed that ERK1, pERK1, AKT and pAKT gene were dramatically enhanced in the miR-126-MSC group compared to the MSC group and the PBS group (both p < 0.05). Echocardiography
showed MiR-126 led to a sustained improvement in cardiac function for at least six weeks at the
injected area, as assessed by left ventricular ejection fraction and fraction of shortening.
Conclusions: Transplantation of MSCs transfected with miR-126 can improve angiogenesis
and cardiac function in the infarcted area of the hearts of mice, which may be due to stimulation
of the AKT/ERK-related pathway. (Cardiol J 2011; 18, 6: 675–681
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