Business and Industry Training Employment Opportunities in Chesapeake, Virginia

The purpose of this study was to determine the number of business and industry training opportunities in Chesapeake, Virginia. To answer this problem, the following questions were established: 1. What are the employment opportunities available in the business and industry training field? 2. What are the job title categories that companies are advertising? 3. In each category of advertised positions, how many people were currently needed at this time and what were the needs projected to 2010? 4. What are the business and industry training employment opportunities for undergraduate degree completers in the Chesapeake, Virginia region

The Perfect Coffee Cup

To design a coffee cup that will maintain the coffee at the optimal drinking temperature for several hours using Phase Change Material, PCM. The importance of this project is designing a coffee cup that will retain its temperature for a prolonged period of time giving the consumer plenty of time to finish their coffee at the desired consumption temperature. We designed a double walled cup that would contain the PCM. Heat transfer calculations were done for different materials such as acrylic, glass, and stainless steel with various thicknesses for each material. These calculations were used to decide on the ideal material the cup should be made out of and the optimum thickness of the material. Rigorous experimentations have been done with the PCM showing the relationship between temperature and time for both melting and cooling the PCM. These experimentations were used to determine the amount of PCM needed per volume of coffee. Based off the preliminary heat transfer calculations, material pricing and availability, it has been concluded to manufacture the cup out of either glass or acrylic for the best results. Once the coffee is poured into the cup, the PCM will begin to melt, immediately activating the PCM. This will instantly cool the coffee to the optimal drinking temperature of 60°C and maintain the temperature of the coffee around 60°C for several hours. We anticipate that the PCM will be able to keep the coffee hot for at least twice as long while, cooling the coffee to drinking temperature in minutes. The PCM will melt at 60°C and will absorb the heat until the temperature of the coffee drops below 60°C. When this happens the PCM will then begin to solidify and radiate heat to warm the coffee back up to optimal temperatures.https://scholarscompass.vcu.edu/capstone/1050/thumbnail.jp

Proteomic analysis of skin invasion by blood fluke larvae.

BackgroundDuring invasion of human skin by schistosome blood fluke larvae (cercariae), a multicellular organism breaches the epidermis, basement membrane, and dermal barriers of skin. To better understand the pathobiology of this initial event in schistosome infection, a proteome analysis of human skin was carried out following invasion by cercariae of Schistosoma mansoni.Methodology and resultsHuman skin samples were exposed to cercariae for one-half hour to two hours. Controls were exposed to water used to collect cercariae in an identical manner, and punctured to simulate cercarial tunnels. Fluid from both control and experimental samples was analyzed by LC/MS/MS using a linear ion trap in "triple play" mode. The coexistence of proteins released by cercariae and host skin proteins from epidermis and basement membrane confirmed that cercarial tunnels in skin were sampled. Among the abundant proteins secreted by cercariae was the cercarial protease that has been implicated in degradation of host proteins, secreted proteins proposed to mediate immune invasion by larvae, and proteins implicated in protection of parasites against oxidative stress. Components of the schistosome surface tegument, previously identified with immune serum, were also released. Both lysis and apoptosis of epidermal cells took place during cercarial invasion of the epidermis. Components of lysed epidermal cells, including desmosome proteins which link cells in the stratum granulosum and stratum spinosum, were identified. While macrophage-derived proteins were present, no mast cell or lymphocyte cytokines were identified. There were, however, abundant immunoglobulins, complement factors, and serine protease inhibitors in skin. Control skin samples incubated with water for the same period as experimental samples ensured that invasion-related proteins and host protein fragments were not due to nonspecific degeneration of the skin samples.ConclusionsThis analysis identified secreted proteins from invasive larvae that are released during invasion of human skin. Analysis of specific host proteins in skin invaded by cercariae served to highlight both the histolytic events facilitating cercarial invasion, and the host defenses that attempt to arrest or retard invasion. Proteins abundant in psoriatic skin or UV and heat-stressed skin were not abundant in skin invaded by cercariae, suggesting that results did not reflect general stress in the surgically removed skin specimen. Abundant immunoglobulins, complement factors, and serine protease inhibitors in skin form a biochemical barrier that complements the structural barrier of the epidermis, basement membrane, and dermis. The fragmentation of some of these host proteins suggests that breaching of host defenses by cercariae includes specific degradation of immunoglobulins and complement, and either degradation of, or overwhelming the host protease inhibitor repertoire

How lamina-associated polypeptide 1 (LAP1) activates Torsin

Lamina-associated polypeptide 1 (LAP1) resides at the nuclear envelope and interacts with Torsins, poorly understood endoplasmic reticulum (ER)-localized AAA+ ATPases, through a conserved, perinuclear domain. We determined the crystal structure of the perinuclear domain of human LAP1. LAP1 possesses an atypical AAA+ fold. While LAP1 lacks canonical nucleotide binding motifs, its strictly conserved arginine 563 is positioned exactly where the arginine finger of canonical AAA+ ATPases is found. Based on modeling and electron microscopic analysis, we propose that LAP1 targets Torsin to the nuclear envelope by forming an alternating, heterohexameric (LAP1-Torsin)[subscript 3] ring, in which LAP1 acts as the Torsin activator. The experimental data show that mutation of arginine 563 in LAP1 reduces its ability to stimulate TorsinA ATPase hydrolysis. This knowledge may help scientists understand the etiology of DYT1 primary dystonia, a movement disorder caused by a single glutamate deletion in TorsinA.National Institute of General Medical Sciences (U.S.) (Award GM103403)United States. Dept. of Energy. Office of Basic Energy Sciences (Contract DE-AC02-06CH11357

Tasting Phenylthiocarbamide (PTC): A New Integrative Genetics Lab with an Old Flavor

First reported in the early 1930s, variation in the ability to taste phenylthiocarbamide (PTC) has since become one of the most widely studied of all human genetic traits. Guo and Reed (2001) provide an excellent review of work on this polymorphism prior to the identification and sequencing of the PTC gene by Kim et al. (2003), and Wooding (2006) provides a stimulating historical review of the role various scientists have played in the study of PTC taste sensitivity and the importance of these studies in relation to natural selection. Identification of the PTC gene and a number of subsequent publications (Wooding et al., 2004; Kim et al., 2005; Wooding et al., 2006) have provided the basis for a new, integrative laboratory investigation of PTC taste sensitivity. This genetics laboratory culminates in the use of the polymerase chain reaction (PCR) and restriction endonuclease digestion to determine the PTC genotype of each student. But “getting there is half the fun” and, in this case, “getting there” requires students to use not only their knowledge of molecular techniques in genetics but also their knowledge of Mendelian genetics, population genetics, probability, and pedigree analysis. The other “half the fun” in this case is that in determining their PTC phenotypes and genotypes, students are learning something about themselves

Household tenure and its associations with multiple long-term conditions amongst working age adults: a cross-sectional analysis using primary care and local government data linked at individual and household levels.

Objectives We quantified associations between household tenure – whether someone privately or socially rents their property, or owner occupies – and prevalence of multiple long-term conditions (MLTCs) amongst working age adults. We also assessed whether the success of data linkages conducted to enable this study introduced potential selection biases. Approach This cross-sectional study used the 2019/20 wave of an innovative dataset linking health and local government data at individual and household levels for residents of an East London borough. To assess potential biases, we calculated standardised differences in variables for matched and unmatched primary care records. Our primary outcome was basic MLTCs, defined as two or more long-term conditions from a list of 38. Two further definitions of MLTCs were operationalised. Multilevel logistic regression was used to explore associations for working age adults (16-64 years, inclusive). Interaction terms were used to evaluate potential interactions between tenure and other household factors. Results Standardised differences in selected health variables for matched and unmatched primary care records were <0.2, indicating selection biases were not introduced due to data linkage success. For participants with successfully linked records, prevalence of basic MLTCs was 18.0%. After adjusting for various sociodemographic, health and socioeconomic variables, odds of basic MLTCs were 36% higher for working age social housing tenants (OR 1.36; 95% CI 1.30-1.42) and 19% lower for private renters (OR 0.81, 95% CI 0.77-0.84) when compared to owner-occupiers. These results were largely consistent across different definitions of MLTCs. Other household-level variables - household benefits receipt, occupancy, and household type – were important modifying factors, with associations between tenure and MLTCs greater for individuals in single adult households and households in receipt of benefits. Conclusion This study demonstrates that primary care and local government data can be linked without introducing selection biases in key health variables and analysed to reveal important insights. We found evidence that household tenure is associated with MLTCs prevalence, emphasising the importance of understanding and addressing household-level social determinants of health

Targeting small molecule drugs to T cells with antibody-directed cell-penetrating gold nanoparticles

We sought to develop a nanoparticle vehicle that could efficiently deliver small molecule drugs to target lymphocyte populations. The synthesized amphiphilic organic ligand-protected gold nanoparticles (amph-NPs) were capable of sequestering large payloads of small molecule drugs within hydrophobic pockets of their ligand shells. These particles exhibit membrane-penetrating activity in mammalian cells, and thus enhanced uptake of a small molecule TGF-β inhibitor in T cells in cell culture. By conjugating amph-NPs with targeting antibodies or camelid-derived nanobodies, the particles' cell-penetrating properties could be temporarily suppressed, allowing targeted uptake in specific lymphocyte subpopulations. Degradation of the protein targeting moieties following particle endocytosis allowed the NPs to recover their cell-penetrating activity in situ to enter the cytoplasm of T cells. In vivo, targeted amph-NPs showed 40-fold enhanced uptake in CD8+ T cells relative to untargeted particles, and delivery of TGF-β inhibitor-loaded particles to T cells enhanced their cytokine polyfunctionality in a cancer vaccine model. Thus, this system provides a facile approach to concentrate small molecule compounds in target lymphocyte populations of interest for immunotherapy in cancer and other diseases.Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (Contract W911NF-13-D-0001)Melanoma Research AllianceNational Cancer Institute (U.S.) (David H. Koch Institute for Integrative Cancer Research at MIT. (Support (Core) Grant P30-CA14051)National Institutes of Health (U.S.) (Grant CA174795)National Institutes of Health (U.S.) (Grant CA172164)Horizon 2020 Framework Programme (European Commission). FutureNanoNeeds Projec