Results of the 2001 Becoming an Outdoor-Woman Survey

INHS Human Dimensions Research Program and Illinois Department of Natural Resourcesunpublishednot peer reviewedOpe

1999-00 Illinois Trapper Survey Report

Federal Aid Project Number W-112-R-9, Job Number 101.2, Wildlife Restoration FundReport issued on: January 5, 200

Register and Charge: Using Synonym Maps to Explore Connotation

To help students think carefully about specific words and their uses, Darren Crovitz and Jessica A. Miller conceive a diagram that visually expresses the spaces and ties between words. Students eagerly explore contextual connotations and defend subtle shifts in word meaning, discovering how time, use, and circumstance all influence meaning

mGlu5 Inhibition in the Basolateral Amygdala Prevents Estrous Cycle-Dependent Changes in Cue-Induced Cocaine Seeking

Drug associated cues are a common relapse trigger for individuals recovering from cocaine use disorder. Sex and ovarian hormones influence patterns of cocaine use and relapse vulnerability, with studies indicating that females show increased cue-induced craving and relapse vulnerability compared to males. In a rodent model of cocaine craving and relapse vulnerability, cue-induced cocaine seeking behavior following weeks of withdrawal from extended-access cocaine self-administration is higher in females in the estrus stage of the reproductive (estrous) cycle (Estrus Females) compared to both Males and females in all other stages (Non-Estrus Females). However, the neuronal substrates and cellular mechanisms underlying these sex differences is not fully understood. One region that contributes to both sex differences in behavioral responding and cue-induced cocaine seeking is the basolateral amygdala (BLA), while one receptor known to play a critical role in mediating cocaine seeking behavior is metabotropic glutamate receptor 5 (mGlu5). Here we assessed the effects of BLA mGlu5 inhibition following prolonged withdrawal from cocaine self-administration on observed estrous cycle-dependent changes in cue-induced cocaine seeking behavior. We found that BLA microinjections of the mGlu5 antagonist MTEP selectively reduced the enhanced cue-induced cocaine seeking normally observed in Estrus Females while having no effect on cocaine seeking in Males and Non-Estrus Females. These findings identify a unique interaction between cocaine-exposure, estrous cycle fluctuations and BLA mGlu5-dependent transmission on cue-induced cocaine seeking behavior

Attitudes of Homeowners in the Greater Chicago Metropolitan Region Towards Nuisance Wildlife

INHS Human Dimensions Research Program and Illinois Department of Natural Resourcesunpublishednot peer reviewedOpe

A Qualitative Study of Workplace Stress and Coping in Secondary Teachers in Ireland

Teacher stress has received scant attention in Ireland. This study examines teachers’ perceptions of their daily stresses and how they attempt to cope with such situations. Interviews were conducted with fifteen secondary teachers from a variety of school types in eastern Ireland. The teachers showed great concern for their students, with some being prepared to ignore school guidelines in order to deal with their pupils’ needs. Several particularly stressful factors were identified, including the maintenance of boundaries (especially when dealing with students with personal problems), dealing with disruptive student behaviour, and the heavy workload. These stresses closely mirrored those described in international literature. Levels of stress and methods of dealing with stress varied widely among the sample, with primary support coming from their colleagues. One strong finding was the lack of suitable training and preparation felt by the participants, particularly in methods of dealing with sensitive and stressful situations in pupils, and in appropriate means of reaction to student misbehaviour. It is recommended that teacher-training courses include explicit training in dealing with such situations

Human Pluripotent Stem Cells Serve as an Effective In Vitro Model for Studies of Early Stages of Retinogenesis

poster abstractSpecification of the neural retina is one of the first events in human development and thus, efforts to study the initial stages of retinal specification have been largely limited. This is particularly true for the earliest event in retinogenesis, the establishment of a definitive retinal fate from a more primitive neural progenitor source. With the advent of human pluripotent stem cells (hPSCs), the complex interplay of transcription factors involved in early events of retinal development from an unspecified pluripotent population can be studied in an in vitro model. To examine this potential, hPSCs were directed to differentiate to a neuroretinal lineage in which a subpopulation of cells adopts a definitive retinal fate whereas others develop toward a forebrain lineage. Samples were collected over the first month of differentiation, starting from the undifferentiated state through when cells acquired either retinal or nonretinal forebrain identities and gene expression patterns were characterized using immunocytochemistry and quantitative RT-PCR. Results demonstrated that while neural transcription factors such as PAX6, OTX2, and LHX2 were expressed early in development, definite retinal transcription factors such as CHX10 were expressed later in differentiation. Furthermore, the expression of CHX10 was found to be uniquely associated with retinal populations and remained absent from the other neural populations, thereby illustrating the ability of this protocol to uniquely identify and isolate retinal populations and further study them in vitro. Overall, these studies will serve to further understand the specification of a retinal fate from a pluripotent population. Such information will assist in the establishment of more efficient methods to generate retinal cells from hiPSCs for translational purposes, as well as establish hiPSCs as a unique in vitro model system for studies of the earliest stages of human development. Mentor: Jason S. Meyer1-

Dominating the Antarctic environment: bryophytes in a time of change

Polar ecosystems, and particularly Antarctica, are one of the few environs in which bryophytes dominate the flora. Their success in these regions is due to bryophytes’ ability to withstand an array of harsh conditions through their poikilohydric lifestyle. However, the unique conditions that allow bryophytes to proliferate over other forms of vegetation also create considerable limitations to growth and photosynthetic activity. High latitude areas are already experiencing some of the most pronounced and rapid climatic change, especially in the Arctic, the Sub-Antarctic Islands and Maritime Antarctica, and these are predicted to continue over the next century. This climatic change is already impacting the flora of the polar regions both via direct and/or indirect impacts on plant species. Water availability and temperature are undoubtedly the most influential factors that determine bryophyte productivity in the Antarctic, but the ozone hole is also having an impact either directly via increased ultraviolet-B radiation and/or indirectly through the increasing wind speeds associated with ozone depletion. In a time of shifting climate the dominance of bryophytes in these regions may be threatened