Volatile Flavor Compounds Vary by Beef Product Type and Degree of Doneness

This study aimed to determine how quality grade and degree of doneness (DOD) influence the development of volatile compounds among beef whole muscle and ground patties. Volatile compounds were quantified via head space solid phase microextraction from samples tempered in refrigerated temperatures (3 to 5 °C), room temperature (24 to 26 °C), or cooked on an electric clamshell-style grill to an endpoint temperature of 55, 60, 71, or 77 °C. Collected samples were subsequently determined by gas chromatography mass spectrometry. Prominent compounds known to be the result of the Maillard reaction or lipid degradation were retained for comparison. Four Strecker aldehydes, 4 pyrazines, and one ester had a 3-way interaction between quality grade, DOD, and product type (each P \u3c 0.001). Pyrazine concentrations did not differ (P \u3e 0.05) in ground patties and was comparably greater (P \u3c 0.05) in steaks; in Prime and Low Choice steaks, pyrazine concentration increased (P \u3c 0.05) as DOD increased. A 2-way interaction between quality grade and product type was observed for acetaldehyde, dimethyl disulfide, 1-penten-3-ol, butanoic acid, hexanal, octanal, nonanal, and 2-heptanone. Among which, octanal and nonanal were greater (P \u3c 0.05) in Prime steaks compared with ground patties. Another 2-way interaction, quality grade and DOD, was observed in 2 ketones, an alcohol, 2 esters, and 2 aldehydes. For example, 2,3-butanedione was greater (P \u3c 0.05) in concentration in Prime 4 °C samples compared with Low Choice and Standard. The final 2-way interaction of DOD and product type was observed in 3 ketones, 2 sulfur compounds, 2 esters, 5 aldehydes, 2 carboxylic acids, and a ketone. For example, 2-heptanone was greater (P \u3c 0.05) in concentration in ground patties compared to steaks in all degrees of doneness except 4 °C. Overall, these results indicate that the volatile flavor profile of beef is greatly influenced by product type and DOD. Generally, consumers select beef based on product type and determine their cookery approach. Therefore, consumers may greatly influence final beef flavor profile

Consumer Liking and Descriptive Flavor Attributes of M. Longissimus Lumborum and M. Gluteus Medius Beef Steaks Held in Varied Packaging Systems

Sensory evaluation was conducted to investigate the impact of packaging system and muscle type on beef flavor. Beef M. Longissimus lumborum (LL) and M. Gluteus medius (GM) steaks were produced from vacuum packaged subprimals at 14 d postmortem. Steaks were placed in various packaging types and held for 7 d prior to 48 h of retail display under continuous fluorescent lighting. Packaging types included: high-oxygen modified atmosphere lidded trays (80% O2/20% CO2, HIOX), carbon monoxide modified atmosphere lidded trays (0.4% CO/30% CO2/69.6%N2, CO), rollstock (forming/non-forming films, ROLL), vacuum packaging (stored in darkness, VAC), and traditional overwrap (foam trays wrapped with polyvinyl chloride film immediately before display, OW). Steaks were evaluated after cooking by consumer panelists and a trained descriptive attribute panel. For consumer evaluation, a muscle × package type interaction (P = 0.040) occurred for juiciness. Overall liking, tenderness, and liking of flavor were influenced (P ≤ 0.003) by package type. Overall liking, liking of flavor, and tenderness of HIOX was lower (P < 0.05) than all other package types. Additionally, package type impacted (P ≤ 0.030) overall, flavor, and tenderness acceptability. Overall and flavor acceptability was the lowest (P < 0.05) for the HIOX treatment. From trained panel attribute evaluation, muscle × package type interactions were detected (P ≤ 0.021) for brown/roasted, bloody/serumy, and juiciness. Juiciness was the least (P < 0.05) desirable for GM HIOX compared to all other muscle and package type combinations. Steaks from HIOX packages had the highest (P < 0.05) ratings for oxidized and cardboardy, and were rated lowest (P < 0.05) in beef flavor ID and umami compared with all other package types. The LL had greater (P < 0.05) beef flavor ID, fat-like, umami, sweet, and overall tenderness ratings than the GM. The GM was more (P < 0.05) liver-like and oxidized compared with the LL. These data indicate a high oxygen packaging environment has the potential to accentuate negative descriptive sensory attributes and lower consumer sensory scores, while diminishing palatability in both muscles

Effects of Dry-Heat Cookery Method on Beef Strip Loin Steaks of Two Quality Grades Following Sous Vide Preparation

The objective of this study was to determine the influence of dry-heat cookery method on beef flavor develop- ment in strip loin steaks from 2 USDA quality grades following sous vide preparation. Beef strip loins were selected from 2 USDA quality grades: upper 2/3 Choice (Modest00–Moderate100) and Select (Slight00–Slight100 marbling, n = 20/grade). Following 21 d of wet aging, strip loins were fabricated into 2.54-cm thick-steaks and randomly assigned to one of 4 dry- heat cookery methods: charbroiler grill (CHAR), clamshell grill (CLAM), convection oven (OVEN), and salamander broiler (SALA). Prior to untrained consumer panel and volatile compound analysis via gas chromatography-mass spectrometry, steaks were cooked under sous vide conditions for 1.5 h, then finished on the assigned cookery method. There were no cooking method ×quality grade interactions (P ≥ 0.076) for all consumer traits evaluated. Overall, SALA steaks received higher (P &lt; 0.05) ratings by consumers than CLAM steaks for all palatability traits. OVEN steaks had greater scores (P &lt; 0.05) than CLAM steaks for juiciness, tenderness, and overall liking but were similar to CLAM steaks (P &gt; 0.05) for flavor. CHAR steaks were similar (P &gt; 0.05) to CLAM steaks for flavor but were rated higher (P &lt; 0.05) for tenderness, juiciness, and overall liking. Steaks cooked using the OVEN method produced a greater concentration of lipid-derived volatiles, such as alcohols, aldehydes, and carboxylic acids. In direct contrast, CHAR steaks produced a higher concentration of pyrazines and Strecker aldehydes, which are derived from the Maillard reaction. These data indicate that cookery method, and therefore heat transfer method, has a substantially stronger influence on consumer ratings and flavor development than USDA quality grade in this study when steaks are prepared using sous vide methods

Evaluation of Dry-Heat Cookery Method on Volatile Flavor Compound Development and Consumer Evaluation of Six Beef Muscles

The objective of this study was to determine the influence of dry-heat cookery on beef flavor development of multiple beef muscles. Beef strip loins, top sirloin butts, paired tenderloins, paired shoulder clods, and chuck rolls were collected from USDA Low Choice carcasses (Small00–Small100 marbling; N = 20). Subprimals were wet aged in the absence of light for 21 d at 0°C to 4°C. Subprimals were fabricated into 2.54-cm-thick steaks representative of the following muscles: Gluteus medius, Infraspinatus, Longissimus lumborum, Psoas major, Serratus ventralis, and Triceps brachii and randomly assigned to one of 4 dry-heat cookery methods: charbroiler grill (CHAR), clamshell grill (CLAM), convection oven (OVEN), and salamander broiler (SALA). Steaks were cooked to a medium degree of doneness (71°C) on the randomly assigned cooking method. Untrained consumer panelists (N = 300) evaluated each sample for flavor, tenderness, juiciness, and overall liking. No interactions were observed between cooking method and muscle (P ≥ 0.344) for any palatability traits evaluated. Consumers preferred CHAR steaks (P &lt; 0.05) over CLAM steaks for flavor, tenderness, juiciness, and overall liking. Additionally, CLAM steaks were rated lower (P &lt; 0.05) than all other methods for tenderness and juiciness. OVEN and SALA steaks were rated higher (P &lt; 0.05) than CLAM steaks by consumers for tenderness and juiciness but were similar (P &gt; 0.05) to CLAM steaks for overall liking. CHAR steaks produced a greater concentration of Maillard compounds compared with the other cooking methods. Steaks cooked using OVEN and SALA (P &lt; 0.05) produced more lipid oxidation products. Additionally, CHAR steaks produced the greatest (P &lt; 0.05) total volatile production compared with all other treatments, which may be a result of the combination of Maillard reaction products and the lipid degradation products

Chemical Characterization and Sensory Relationships of Beef M. longissimus lumborum and M. gluteus medius Steaks After Retail Display in Various Packaging Environments

Volatile compounds, carbonyls, non-heme iron, and thiobarbituric acid reactive substances (TBARS) were measured in both raw and cooked beef samples to determine the effects of muscle and packaging type on beef flavor development. All paired strip loins and top sirloin butts were packaged under vacuum and aged for 14 d postmortem. After initial aging, all subprimals were fabricated to produce M. gluteus medius (GM) or M. longissimus lumborum (LL) steaks. At 14 d postmortem, steaks were randomly assigned to 1 of 5 package types: high-oxygen modified atmosphere lidded trays (80% O2/20% CO2 [“HIOX”]), carbon monoxide modified atmosphere lidded trays (0.4% CO/30% CO2/69.6% N2 [“CO”]), rollstock (forming and non-forming films [“ROLL”]), vacuum packaging without retail display (“VAC”), and traditional overwrap (“OW”) remained under vacuum prior to retail display. Steaks were stored in darkness an additional 7 d prior to display. At 21 d postmortem, HIOX, OW, CO, and ROLL packages were displayed for 48 h under continuous fluorescent lighting, while VAC steaks remained in dark storage. Packaging and muscle type impacted (P &lt; 0.05) quantities of multiple volatile flavor compounds, including alcohols, n-aldehydes, esters, furans, hydrocarbons, sulfur-containing compounds,and ketones in both raw and cooked samples. Volatile compounds related to lipid oxidation were more (P &lt; 0.05) prominent in HIOX packaging. Package type (P &lt; 0.05) and muscle (P &lt; 0.05) had an impact on raw-steak TBARS, although package type did not influence (P &gt; 0.05) cooked-steak TBARS. The GM possessed greater (P &lt; 0.05) TBARS values than the LL in both raw and cooked samples. Package type had no effect (P &gt; 0.05) on carbonyl and non-heme iron content although these analyses differed among muscles (P &lt; 0.05), with the GM being greater (P &lt; 0.05) than the LL. These results indicate that the development of lipid oxidation that occurs during storage and display was muscle and packaging specific. Therefore, to maintain flavor, quality packaging systems should be selected on a muscle-specific basis

Cooking Method and USDA Quality Grade Affect Consumer Palatability and Flavor of Beef Strip Loin Steaks

Consumer sensory analysis (n = 288) was conducted, along with cooking loss, slice shear force (SSF), pressed juice percentage (PJP), and volatile compound analyses, to evaluate the effects of different dry heat cooking methods. Specifically, an electric clamshell grill (CLAM), flat top gas grill (FLAT), charbroiler gas grill (CHAR), and salamander gas broiler (SAL) were used to cook beef strip loin steaks from 4 USDA quality grades [Prime, Top (upper 2/3) Choice, Low (lower 1/3) Choice, and Select)] to determine the palatability. Cooking method and quality grade influenced (P 0.05) SSF or PJP. Both cooking method and quality grade impacted (P < 0.05) the headspace concentration of some volatile compounds in the alcohol, n-aldehyde, Strecker aldehyde, and furan groups. Cooking method also affected all pyrazines, and quality grade had an effect on 1 ketone (P < 0.05). These results indicate cooking method had a significant impact on consumer palatability ratings and objective measures of beef flavor, and those results were consistent across a range of quality grades

The Impact of Enhancement, Degree of Doneness, and USDA Quality Grade on Beef Flavor Development

The objective of this study was to determine the impact of enhancement, degree of doneness (DOD), and USDA quality grade on beef volatile flavor compounds from cooked strip loin steaks. This study also aimed to evaluate relationships between volatile compounds and consumer sensory responses. Beef strip loins (n = 72; 24/grade) of 3 quality grades (USDA Prime, Low Choice, and Low Select) were enhanced (8% of green weight with brine containing 0.35% salt and 0.4% sodium phosphate) or not enhanced, and cooked to 3 DOD (Rare: 60°C; Medium: 71°C; Very Well Done: 83°C) before volatile analysis. Volatile compounds were evaluated through a split-plot design where enhancement level and quality grade were used as the whole plot factors and DOD served as the subplot factor. Principal component analysis (PCA) was utilized to explore relationships between volatile compounds, consumer response, and treatments. The majority of compounds (n = 32) were impacted (P 0.05). In agreement, PCA indicated volatile compound production was primarily driven by degree of doneness and quality grade. There was no strong link between enhancement and beef volatile flavor compound development, despite the dramatically improved flavor liking scores from consumers

Evaluation of Beef Steak Flavor Development in Vacuum Rollstock Packaging Under Two Lighting Sources

The objective of this study was to determine the influence of lighting type and display duration on flavor development in multiple beef muscles. Paired beef top sirloin butts, strip loins, and tenderloins were collected from USDA Low Choice carcasses (Small00 to Small100 marbling score, n=16). Subprimals were wet aged in the absence of light for 7 d postmortem at 0°C to 4°C before being fabricated into 2.5-cm steaks representing the Gluteus medius, Longissimus lumborum (LL), and Psoas major. Steaks were packaged individually in vacuum rollstock packaging and assigned to either light-emitting diode (LED) or fluorescent (FLUR) display cases for a display period of 0, 2, 6, or 10 d. All steaks were assigned to either trained descriptive panel analysis (n=384) or volatile compound analysis (n=384) and cooked to a medium degree of doneness (71°C). Two-way interactions occurred between lighting type and display duration, showing increased tenderness sooner during display for LED steaks, and lower umami intensity in FLUR steaks after 10 d (P&lt;0.001). Lighting and muscle type showed more tender LL and Psoas major steaks in LED lighting (P≤0.001). Lighting and display duration interactions also showed increased concentrations of 2,3-butanedione under FLUR light and ethyl benzene under LED display (P≤0.043), whereas lighting and muscle type showed greater concentrations of alcohols and carboxylic acids in LL steaks under LED lighting (P≤0.046). Furthermore, discriminant function analyses were per-formed, suggesting that the most successful retail display period was within 2 to 6 d, with no difference between lighting types (P=0.212). Overall, these data reveal little differentiation between lighting types, implying that newer LED lighting does not detrimentally influence beef quality when vacuum packaging is utilized

A Comparison of Consumer Responses Using Paper and Digital Ballots for Eating Quality Assessment of Beef Steaks

A consumer study was conducted to determine whether consumers scored beef palatability traits differently on paper versus digital ballots. Beef subprimals representing 4 treatments with inherent variation in eating quality were collected: USDA Select eye of round aged 7 d postmortem, USDA Select strip loin aged 7 d postmortem, USDA Choice tenderloin aged 21 d postmortem, and USDA Prime strip loin aged 21 d postmortem. Accessory muscles, external fat, and connective tissue were removed from subprimals. Muscles were fabricated into 2.5-cm steaks and further divided into 2 equal halves for consumer testing. Consumers (n = 360) evaluated 8 samples divided into 2 blocks representing the 2 ballot types. Within each ballot block, Select longissimus lumborum samples were always served in the first and fifth position, followed by the remaining 3 treatments served in a randomized order among the latter 3 positions. Consumers rated each steak sample for tenderness, juiciness, flavor liking, and overall liking from 0 to 100 on either a paper or a digital ballot and then rated the paired steak halves on the opposite ballot during the second block of sample testing. Ballot type influenced (P &lt; 0.02) all traits, as consumers scored traits greater (P &lt; 0.05) on paper compared with digital ballots, regardless of treatment. The magnitude of differences between ballot types was much smaller than the magnitude of differences between cut treatments, which also differed (P &lt; 0.01). The smallest margin between ballot type was observed for tenderness (1.8 points); juiciness, flavor liking, and overall liking all differed by 3.4 points. Independent studies could and have utilized digital ballots without concern, as consumers sorted samples by treatment in the current study similarly, regardless of ballot type. However, researchers should consider ballot type for their sensory studies, especially if data will be added to a collective data set

Investigation of Smoked Beef Brisket Palatability from Three USDA Quality Grades

The objective of this study was to investigate differences in smoked beef brisket palatability from 3 USDA quality grades (USDA Prime, average [middle 1/3] Choice, and Select). Briskets (n = 54; 18 per quality grade) were seasoned with a blend of 1:1 coarse salt/black pepper, and then cooked in a smoker to a final internal temperature of 93°C for approximately 6 to 7 h. For sensory analysis, briskets were separated into point (pectoralis superficialis) and flat (pectoralis profundus) portions and then sliced perpendicular to the muscle fibers. Consumer panelists (N = 360) evaluated palatability traits, acceptability of each trait, and willingness to pay (WTP). An interaction between quality grade and muscle was observed (P ≤ 0.03) for all palatability traits, proportion of acceptable samples, and WTP. Consumers could not distinguish among quality grades of the point portions for tenderness, juiciness, flavor liking, and overall liking (P &gt; 0.05). Point samples, regardless of quality grade, were scored greater (P &lt; 0.05) than Prime flat samples for all palatability traits. Choice and Select flat samples were scored lesser (P &lt; 0.05) than all other treatment combinations for tenderness, flavor liking, and overall liking. In alignment with palatability traits, consumers’ WTP was greatest for point portions, regardless of quality grade (P &lt; 0.05), followed by Prime flat portions. Choice and Select flat portions had the lowest WTP (P &lt; 0.05). Consumer acceptability of cooked beef brisket generally followed similar trends as palatability scores. Quality grade had no effect on the eating quality of the point portions of smoked briskets, and point portions received superior palatability scores to flat portions. Prime flat portions had greater eating quality compared to that of Choice and Select flat portions, and consumers had greater WTP for what they perceived as superior eating quality